Protein extraction methods for the two-dimensional gel electrophoresis analysis of the slow growing fungus Undifilum oxytropis

2012 ◽  
Vol 6 (4) ◽  
Author(s):  
Haili Li
Keyword(s):  
Gel Electrophoresis ◽  
Protein Extraction ◽  
Extraction Methods ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Slow Growing

Protein extraction from mature rice leaves for two-dimensional gel electrophoresis and its application in proteome analysis

PROTEOMICS ◽  
2004 ◽  
Vol 4 (7) ◽  
pp. 1903-1908 ◽  
Author(s):  
Nazrul Islam ◽  
M. Lonsdale ◽  
N. M. Upadhyaya ◽  
T. J. Higgins ◽  
H. Hirano ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Protein Extraction ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Rice Leaves

scholarly journals Optimized Protocol for Protein Extraction from the Breast Tissue that is Compatible with Two-Dimensional Gel Electrophoresis

2011 ◽  
Vol 5 ◽  
pp. BCBCR.S6263 ◽  
Author(s):  
Olena Zakharchenko ◽  
Christina Greenwood ◽  
Louise Alldridge ◽  
Serhiy Souchelnytskyi
Keyword(s):  
Gel Electrophoresis ◽  
Breast Tissue ◽  
Protein Extraction ◽  
Small Sample Size ◽  
Small Sample ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Main Challenge ◽  
Optimized Protocol ◽  
Separation Of Proteins

Proteomics is a highly informative approach to analyze cancer-associated transformation in tissues. The main challenge to use a tissue for proteomics studies is the small sample size and difficulties to extract and preserve proteins. The choice of a buffer compatible with proteomics applications is also a challenge. Here we describe a protocol optimized for the most efficient extraction of proteins from the human breast tissue in a buffer compatible with two-dimensional gel electrophoresis (2D-GE). This protocol is based on mechanically assisted disintegration of tissues directly in the 2D-GE buffer. Our method is simple, robust and easy to apply in clinical practice. We demonstrate high quality of separation of proteins prepared according to the reported here protocol.

scholarly journals Protein extraction method of Metroxylon sagu leaf for high resolution two dimensional gel electrophoresis and comparative proteomics

2019 ◽  
Author(s):  
Mehvish Nisar ◽  
Hasnain Hussain
Keyword(s):  
Gel Electrophoresis ◽  
Extraction Method ◽  
Protein Extraction ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Fractionation Method ◽  
Metroxylon Sagu ◽  
Time Required

Abstract Objective This study aimed to determine the best protein extraction method of Metroxylon sagu for the two-dimensional gel electrophoresis and the comparative analysis. Results To perform good proteome research, the most critical step is to establish a method that gives the best quality of extracted total proteins. To develop an optimized protein extraction protocol for two-dimensional polyacrylamide gel electrophoresis (2-DE) analysis of Metroxylon sagu, five protein extraction protocols were compared; polyethene glycol (PEG) fractionation method, SDS/phenol method, TCA/acetone method, combination SDS/phenol and TCA/acetone and imidazole method. The PEG fractionation method was found to give the most reproducible gels with the highest number of spots and highest protein concentration followed by SDS/Phenol method. The lowest number of spots were observed in Imidazole method. The PEG fractionation method provides improved resolution and reproducibility of two-dimensional polyacrylamide gel electrophoresis (2-DE) and reduces the time required to analyze samples. Partitioning rubisco by polyethene glycol (PEG) fractionation provides clearer detection of low-abundance protein. Hence the result from this study propose PEG fractionation as the effective protein extraction method for 2-DE proteomic studies of Metroxylon sagu.

scholarly journals Comparison and optimization of protein extraction and two-dimensional gel electrophoresis protocols for liverworts

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Sandhya Yadav ◽  
Akanksha Srivastava ◽  
Subhankar Biswas ◽  
Neha Chaurasia ◽  
Sushil Kumar Singh ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Protein Extraction ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis
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