Characterization of Vibrio alginolyticus and Vibrio parahaemolyticus isolated from Penaeus monodon: Antimicrobial resistance, plasmid profiles and random amplification of polymorphic DNA analysis

2012 ◽  
Vol 6 (20) ◽  
Author(s):  
Nilima Priyadarshini Marhual
Keyword(s):  
Antimicrobial Resistance ◽  
Vibrio Parahaemolyticus ◽  
Dna Analysis ◽  
Penaeus Monodon ◽  
Vibrio Alginolyticus ◽  
Plasmid Profiles ◽  
Resistance Plasmid ◽  
Random Amplification

scholarly journals Random Amplification of Polymorphic DNA Analysis for Genetic Characterization of Two Breeds of Dogs, German Shepherd and Japanese Spitz

2013 ◽  
Vol 13 (2) ◽  
pp. 73-78
Author(s):  
Jarina Joshsi ◽  
Lumanti Manandhar ◽  
Patima Shrestha ◽  
Rani Gupta ◽  
Rojlina Manadhar ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Dna Analysis ◽  
Genetic Characterization ◽  
Random Amplified Polymorphic Dna ◽  
Neighbor Joining ◽  
German Shepherd ◽  
Polymorphic Loci ◽  
Random Amplification

Random amplified polymorphic DNA (RAPD) markers were used to study genetic diversity in dog samples belonging to populations of German Shepherd and Japanese Spitz. A total of twelve samples were typed using eight RAPD primers. Out of eight primers, three primers gave result in six individuals of dogs. The phylogenetic tree constructed by the neighbor joining method based on Nei. Original measures revealed highest genetic identity found in German Shepherd as 0.9444 and highest genetic distance as 1.2809. The analysis predicts the number of polymorphic loci as 15 and the percentage of polymorphic loci as 83.3. Nepal Journal of Science and Technology Vol. 13, No. 2 (2012) 73-78 DOI: http://dx.doi.org/10.3126/njst.v13i2.7717

scholarly journals Characterization of Antimicrobial Resistance and Virulence Profile of Vibrio Parahaemolyticus in Water and Bivalve Mollusck Samples

2014 ◽  
Author(s):  
Norma Suely Evangelista-Barreto ◽  
Irana Paim Silva ◽  
Carla da Silva Silveira ◽  
Leopoldo Melo Barreto ◽  
Oscarina Viana de Sousa
Keyword(s):  
Antimicrobial Resistance ◽  
Vibrio Parahaemolyticus

scholarly journals MULTIDRUG RESISTANCE (MDR) OF V. Parahaemolyticus

2015 ◽  
Vol 2 (2) ◽  
pp. 112
Author(s):  
Marlina
Keyword(s):  
United States ◽  
Antimicrobial Resistance ◽  
Vibrio Parahaemolyticus ◽  
Antimicrobial Agents ◽  
The United States ◽  
Food Microbiology ◽  
Aeromonas Species ◽  
General Strategy ◽  
Rapd Pcr

 Vol. 2, No. 2 ABSTRACT A total of 97 V. parahaemolyticus isolate from Padang were examined for their resistance to 15 antibiotics. V. parahaemolyticus isolated behaved as resistant to sulfamethoxazole (100%), rifampin (95%) and tetracycline (75%) and sensitive to norfloxacin (96%). Ampicillin still sensitive for V. parahaemolyticus isolated from human stools. All of isolates were sensitive to namely chloramphenicol and floroquinolones (ciprofloxacin and norfloxacin agents). RAPD-PCR profiling with three primers (OPAR3, OPAR4 and OPAR8) produced four major clusters (R1, R2, R3 and R4), 7 minor clusters (I, II, III, IV, V, VI and VII) and three single isolates.  Keywords: V. parahaemolyticus, MDR, RAPD  1. D. Ottaviani, I. Bacchiocchi, L. Masini, F. Leoni, A. Carraturo, M. Giammarioli, and G. Sbaraglia, Antimicrobial susceptibility of potentially halophilic vibrios isolated from seafood, International Journal of Antimicrobial Agents 18: 135-140, (2001).2. A. Cespedes, and E. Larson, Knowledge, attitude and practices regarding antibiotic use among Latinos in the United States: Review and Recommendations, American Journal of Infection Control 34: 495-502, (2006).3. M. Lesmana, D. Subekti, C.H. Simanjuntak, P. Tjaniadi, J. R. Campbell, and B. A. Ofoyo, Vibrio parahaemolyticus associated with cholera-like diarrhea among patients in North Jakarta, Indonesia, Diagnostic Microbiology and Infectious Disease, 39: 71-75, (2001).4. S. Lu, B. Liu, B. Zhou, And R. E. Levin, Incidence and Enumeration of Vibrio parahaemolyticus in Shellfish from two retail Sources and the Genetic Diversity of isolates as Determined by RAPD-PCR Analysis, Food Biotechnology, 20: 193-209, (2006).5. M. Nishibuchi, Vibrio parahaemolyticus. In International handbook of foodborne pathogens, ed. M.D. Milliots and J. W. Bier, United States: Marcel Dekker, Inc. P, 2004, 237-252.6. L. Poirel, M. R. Martinez, H. Mammeri, A. Liard, and P. Nordmann, Origin of Plasmid-Mediated Quinolone Resistance Determinant QnrA, Antimicrobial Agents and Chemotherapy, 49: 3523-3525, (2005).7. S. Radu, N. Elhadi, Z. Hassan, G. Rusul, S. Lihan, N. Fifadara, Yuherman and E. Purwati, Characterization of Vibrio vulnificus isolated from cockles (Anadara granosa): antimicrobial resistance, plasmid profiles and random amplification            of polymorphic DNA analysis, FEMS Microbiology Letters, 165: 139–143, (1998).8. S. Radu, N. Ahmad, F. H. Ling, and A. Reezal, Prevalence and resistance             to antibiotics for Aeromonas species from retail fish in Malaysia, International of Journal Food Microbiology, 81: 261–266, (2003).9. B. Sarkar, N. R. Chowdhury, G. B. Nair, M. Nishibuchi, S. Yamasaki, Y. Takeda, S. K. Gupta, S. K. Bhattacharya, and Ramamurthy, Molecular characterization of Vibrio parahaemolyticus of similar serovars isolated from sewage and clinical cases of diarrhea in Calcutta, India,   World Journal of Microbiology and Biotechnology, 19: 771-776, (2003). 10. S. Schwarz, and E. Chaslus-Dancla, Use of antimicrobials in veterinary medicine and mechanisms of resistance, Veterinary Residue, 32: 201–225, (2001).11. H. Sörum, and T.M. L’Abèe-Lund,.  Antibiotic resistance in food-related bacteria – a result of interfering with the global web of bacterial genetics, International Journal of Food Microbiology, 78: 43–56, (2002).12. P. Tjaniadi, M. Lesmana, D. Subekti, N. Machpud, S. Komalarini, W. Santoso,     C. H. Simanjuntak, N. Punjabi, J. R. Campbell, W. K. Alexander, H. J. Beecham, A. L. Corwin, and B. A. Oyofo, Antimicrobial Resistance of Bacterial Pathogens Associated with Diarrheal Patients in Indonesia, American Journal   of Tropical Medicine and Hygiene,             68: 666-670, (2003).13. X. Zhao, and D. Drlica, Restricting              the Selection of Antibiotic-Resistant Mutants: A General Strategy Derived     from Fluoroquinolone Studies, Clinical Infectious Diseases, 33: S147-S156, (2001).   

Characterization of Serpulina hyodysenteriae isolates of serotypes 8 and 9 by random amplification of polymorphic DNA analysis

1996 ◽  
Vol 48 (3-4) ◽  
pp. 305-314 ◽  
Author(s):  
Dominique Dugourd ◽  
Mario Jacques ◽  
Michel Bigras-Poulin ◽  
Josée Harel
Keyword(s):  
Dna Analysis ◽  
Serpulina Hyodysenteriae ◽  
Random Amplification

scholarly journals Characterization of Pathogenic Vibrio parahaemolyticus Isolated From Fish Aquaculture of the Southwest Coastal Area of Bangladesh

2021 ◽  
Vol 12 ◽  
Author(s):  
Abu Baker Siddique ◽  
M. Moniruzzaman ◽  
Sobur Ali ◽  
Md. Nayem Dewan ◽  
Mohammad Rafiqul Islam ◽  
...  
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Pearson Correlation ◽  
Penaeus Monodon ◽  
Foodborne Pathogen ◽  
Virulence Gene ◽  
Economic Losses ◽  
Ileal Loop ◽  
Virulence Potential ◽  
Pathogenic Vibrio

Vibrio parahaemolyticus is a major foodborne pathogen responsible for significant economic losses in aquaculture and a threat to human health. Here, we explored the incidence, virulence potential, and diversity of V. parahaemolyticus isolates from aquaculture farms in Bangladesh. We examined a total of 216 water, sediment, Oreochromis niloticus (tilapia), Labeo rohita (rui), and Penaeus monodon (shrimp) samples from the aquaculture system where 60.2% (130/216) samples were positive for V. parahaemolyticus. Furthermore, we identified 323 V. parahaemolyticus strains from contaminated samples, 17 of which were found positive for trh, a virulence gene. Four isolates out of the 17 obtained were able to accumulate fluid in the rabbit ileal loop assay. The correlation between the contamination of V. parahaemolyticus and environmental factors was determined by Pearson correlation. The temperature and salinity were significantly correlated (positive) with the incidence of V. parahaemolyticus. Most of the pathogenic isolates (94.1%) were found resistant to ampicillin and amoxicillin. O8: KUT was the predominant serotype of the potentially pathogenic isolates. ERIC-PCR reveals genetic variation and relatedness among the pathogenic isolates. Therefore, this region-specific study establishes the incidence of potential infection with V. parahaemolyticus from the consumption of tilapia, rui, and shrimp raised in farms in Satkhira, Bangladesh, and the basis for developing strategies to reduce the risk for diseases and economic burden.

scholarly journals Incidence, Antibiotic Susceptibility and Characterization of Vibrio parahaemolyticus Isolated from Seafood in Selangor, Malaysia

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Vurmila Venggadasamy ◽  
Teng Hern Loh ◽  
Jodi Woan Fei Law ◽  
Hooi-Leng Ser ◽  
Vengadesh Letchumanan ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Foodborne Pathogens ◽  
Antibiotic Susceptibility ◽  
Vibrio Parahaemolyticus ◽  
Species Level ◽  
Potential Health ◽  
Potential Health Risk ◽  
Plasmid Profiles ◽  
Toxr Gene

Vibrio parahaemolyticus is one of the major foodborne pathogens owing to its cause of infectious diseases such as gastroenteritis. These diseases are often associated with the consumption of contaminated seafood. This study aims to investigate the presence of V. parahaemolyticus, their virulence, antibiotic profiles, and plasmid profiles from 77 different kinds of shellfish samples collected from wet markets and supermarkets in Selangor, Malaysia. High densities of Vibrio species ( > 5 log CFU/g) were found in 14/16 groups of shellfish. Among 77 presumptive V. parahaemolyticus isolates, 43 (55.8%) were positive for the toxR gene, confirming the identity of the isolates at the species level. However, none of the V. parahaemolyticus isolates harboured the virulence tdh and trh genes. The antibiotic susceptibility of the V. parahaemolyticus isolates revealed that most of them were resistant to ampicillin (95.3%), ampicillin-sulbactam (81.4%), cefotaxime (37.2%) and imipenem (23.3%). The plasmid profiles of the V. parahaemolyticus isolates showed that 41.9% (18/43) possess at least one plasmid. Our results indicate the V. parahaemolyticus isolates are continuously exposed to various antibiotics in the environments, thus consuming the seafood carries a potential health risk to consumers. The antibiotic resistance conferred by the species necessitates an immediate plan to approach the usage of antibiotics differently. 

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