Isolation and identification of Bacillus strains with antimycobacterial activity

2011 ◽  
Vol 5 (21) ◽  
Author(s):  
Souraya El Guendouzi
Keyword(s):  
Antimycobacterial Activity ◽  
Isolation And Identification ◽  
Bacillus Strains

scholarly journals Diversity and Antimicrobial Activity of Culturable Fungal Endophytes in Solanum mauritianum

2020 ◽  
Vol 17 (2) ◽  
pp. 439 ◽  
Author(s):  
Sharon Pelo ◽  
Vuyo Mavumengwana ◽  
Ezekiel Green
Keyword(s):  
Deoxyribonucleic Acid ◽  
Fungal Endophytes ◽  
Antimycobacterial Activity ◽  
Great Activity ◽  
Isolation And Identification ◽  
Gram Positive Bacteria ◽  
Crude Extracts ◽  
Solanum Mauritianum ◽  
Fusarium Sp ◽  
Respective Host

Plant endophytes are microbial sources of bioactive secondary metabolites, which mimic the natural compounds chemistry of their respective host plants in a similar manner. This study explored the isolation and identification of fungal endophytes, and investigated the antibacterial and antimycobacterial activity of their crude extracts. Fungal endophytes were isolated from Solanum mauritianum, identified using morphological traits and internal transcribed spacer ribosomal-deoxyribonucleic acid (ITS-rDNA) sequence analysis. Eight fungal endophytes were identified as Aureobasidium pullulans, Paracamarosporium leucadendri, Cladosporium sp., Collectotrichum boninense, Fusarium sp., Hyalodendriella sp., and Talaromyces sp., while Penicillium chrysogenum was isolated from the leaves and unripe fruits. Good activity was observed for the crude extracts of Paracamarosporium leucadendri inhibiting Mycobacterium bovis, Klebsiella pneumoniae, and Pseudomonas aeruginosa at 6 µg/mL. Crude extracts of Fusarium sp., showed activity at 9 μg/mL against M. bovis, M. smegmatis and K. pneumonia. In general, the crude extracts showed great activity against Gram-negative and Gram-positive bacteria and novel results for two mycobacteria species M. bovis and M. smegmatis. The results provide evidence of diverse fungal endophytes isolated from Solanum mauritianum, and evidence that fungal endophytes are a good source of bioactive compounds with pharmaceutical potential, particularly against Mycobacterium tuberculosis.

scholarly journals Isolation and Identification of Bacillus Strains with Antagonistic Properties against Film-forming Yeasts Overgrown in Low Salted Soybean Pastes

2013 ◽  
Vol 49 (3) ◽  
pp. 286-291 ◽  
Author(s):  
SaeBom Jeon ◽  
MyeongSeon Ryu ◽  
Yong Sang Kim ◽  
Seung Wha Jo ◽  
Do Yeon Jeong ◽  
...  
Keyword(s):  
Isolation And Identification ◽  
Film Forming ◽  
Bacillus Strains

scholarly journals Isolation and Identification of the Potential Novel L-asparaginase producing Bacillus Strains from Soil

2021 ◽  
pp. 11-19
Author(s):  
Naushaba Nazli ◽  
Rukhsar Masood ◽  
Muhmmad Salman ◽  
Bilal Nasir ◽  
Farah Shireen ◽  
...  
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Agricultural Land ◽  
Lymphoblastic Leukemia ◽  
Dairy Farm ◽  
Sequencing Analysis ◽  
Phenol Red ◽  
Isolation And Identification ◽  
Isolation And Characterization ◽  
Asparaginase Activity ◽  
Bacillus Strains

L-Asparaginase is a well know enzyme for its antineoplastic potential and is widely used to treat acute lymphoblastic leukemia and lymphosarcoma. The present work describes the isolation and characterization of novel L-asparaginase producing Bacillus strains from soil. Soil samples were collected from three different locations such as fruit garden, dairy farm and agricultural land in Peshawar Khyber Pakhtunkhwa, Pakistan. The isolates were screened to produce L-asparaginase in growth medium supplemented with 1% L-asparagine using a phenol red indicator. Among 30 bacterial isolates, only two strains initially coded as A5 and FG7 showed L-asparaginase activity. Based on biochemical and 16S rRNA sequencing analysis, the isolate A5 and FG7 were identified as Bacillus amyloliquefaciens and Bacillus proteolyticus respectively. Different factors like pH and time were optimized for maximum L-asparaginase activity. Bacillus amyloliquefaciens showed maximum asparaginase activity at pH 7 after 24 hours incubation at 30oC, while Bacillus proteolyticus showed optimum activity at pH 7 after 48 hours of incubation at 30oC. The present study first time reported the production of L-asparginase enzyme from Bacillus amyloliquefaciens and Bacillus proteolyticus. Keywords: L-asparaginase, Bacillusamyloliquefaciens, Bacillus proteolyticus, 16sRNA.

Antimycobacterial activity of medicinal plants from Mozambique

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
G Da Silva ◽  
A Macedo ◽  
I Famba ◽  
M Taniça ◽  
R Serrano ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Antimycobacterial Activity

Isolation and identification of chemical constituents from the roots of Peucedanum praeruptorum

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
YJ Lee ◽  
J Kim ◽  
J Lee ◽  
ES Cho ◽  
OS Bang
Keyword(s):  
Chemical Constituents ◽  
Isolation And Identification
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