Production and characterisation of protease enzyme produced by a novel moderate thermophilic bacterium (EP1001) isolated from an alkaline hot spring, Zimbabwe

2012 ◽  
Vol 6 (27) ◽  
Author(s):  
Parawira Wilson
Keyword(s):  
Hot Spring ◽  
Thermophilic Bacterium ◽  
Protease Enzyme

scholarly journals Draft Genome Sequence of Thermophilic Halotolerant Aeribacillus pallidus TD1, Isolated from Tao Dam Hot Spring, Thailand

2019 ◽  
Vol 8 (17) ◽  
Author(s):  
Wariya Yamprayoonswat ◽  
Satapanawat Sittihan ◽  
Watthanachai Jumpathong ◽  
Montri Yasawong
Keyword(s):  
Genome Sequence ◽  
Hot Spring ◽  
Draft Genome ◽  
Thermophilic Bacterium ◽  
Draft Genome Sequence ◽  
Naphthalene Dioxygenase ◽  
Content Type ◽  
Naphthalene Degradation ◽  
Key Enzyme

Aeribacillus pallidus TD1 is a thermophilic bacterium isolated from a hot spring in Thailand. The genome sequence of A. pallidus TD1 contains a gene-encoded naphthalene dioxygenase, which is a key enzyme for naphthalene degradation.

scholarly journals Insights into Brevibacillus borstelensis AK1 through Whole Genome Sequencing: A Thermophilic Bacterium Isolated from a Hot Spring in Saudi Arabia

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Amjad B. Khalil ◽  
Neelamegam Sivakumar ◽  
Muhammad Arslan ◽  
Hamna Saleem ◽  
Sami Qarawi
Keyword(s):  
Saudi Arabia ◽  
Hot Spring ◽  
Gc Content ◽  
Morphological Characterization ◽  
Thermophilic Bacterium ◽  
Whole Genome Sequence ◽  
Strictly Aerobic ◽  
Whole Genome ◽  
Aerobic Bacterium ◽  
Wide Range

Brevibacillus borstelensis AK1 is a thermophile which grows between the temperatures of 45°C and 70°C. The present study is an extended genome report of B. borstelensis AK1 along with the morphological characterization. The strain is isolated from a hot spring in Saudi Arabia (southeast of the city Gazan). It is observed that the strain AK1 is rod-shaped, motile, and strictly aerobic bacterium. The whole genome sequence resulted in 29 contigs with a total length of 5,155,092 bp. In total, 3,946 protein-coding genes and 139 RNA genes were identified. Comparison with the previously submitted strains of B. borstelensis strains illustrates that strain AK1 has a small genome size but high GC content. The strain possesses putative genes for degradation of a wide range of substrates including polyethylene (plastic) and long-chain hydrocarbons. These genomic features may be useful for future environmental/biotechnological applications.

Degradation of poly(ε-caprolactone) by a thermophilic bacterium Ralstonia sp. strain MRL-TL isolated from hot spring

2015 ◽  
Vol 98 ◽  
pp. 35-42 ◽  
Author(s):  
Aamer Ali Shah ◽  
Ahmed Nawaz ◽  
Lubna Kanwal ◽  
Fariha Hasan ◽  
Samiullah Khan ◽  
...  
Keyword(s):  
Hot Spring ◽  
Thermophilic Bacterium

Brockia lithotrophica gen. nov., sp. nov., an anaerobic thermophilic bacterium from a terrestrial hot spring

2013 ◽  
Vol 63 (Pt_2) ◽  
pp. 479-483 ◽  
Author(s):  
Anna A. Perevalova ◽  
Ilya V. Kublanov ◽  
R. V. Baslerov ◽  
Gengxin Zhang ◽  
Elizaveta A. Bonch-Osmolovskaya
Keyword(s):  
Hot Spring ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Thermophilic Bacterium ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
Content Type ◽  
Positive Type ◽  
Link Type ◽  
Terrestrial Hot Spring

A novel thermophilic bacterium, strain Kam1851T, was isolated from a terrestrial hot spring of the Uzon Caldera, Kamchatka Peninsula, Russia. Cells of strain Kam1851T were spore-forming rods with a Gram-positive type of cell wall. Growth was observed between 46 and 78 °C, and pH 5.5–8.5. The optimal growth (doubling time, 6.0 h) was at 60–65 °C and pH 6.5. The isolate was an obligate anaerobe growing in pre-reduced medium only. It grew on mineral medium with molecular hydrogen or formate as electron donors, and elemental sulfur, thiosulfate or polysulfide as electron acceptors. The main cellular fatty acids were C16 : 0 (34.2 %), iso-C16 : 0 (18 %), C18 : 0 (12.8 %) and iso-C17 : 0 (11.1 %). The G+C content of the genomic DNA of strain Kam1851T was 63 mol%. 16S rRNA gene sequence analysis showed that strain Kam1851T belonged to the order Thermoanaerobacterales , but it was not closely related to representatives of any genera with validly published names. The most closely related strains, which had no more than 89.2 % sequence similarity, were members of the genera Ammonifex and Caldanaerobacter . On the basis of its phylogenetic position and novel phenotypic features, isolate Kam1851T is proposed to represent a novel species in a new genus, Brockia lithotrophica gen. nov., sp. nov.; the type strain of Brockia lithotrophica is Kam1851T ( = DSM 22653T = VKM B-2685T).

Anoxybacillus sediminis sp. nov., a novel moderately thermophilic bacterium isolated from a hot spring

2018 ◽  
Vol 111 (12) ◽  
pp. 2275-2282 ◽  
Author(s):  
Inam Ullah Khan ◽  
Neeli Habib ◽  
Min Xiao ◽  
Asem Mipeshwaree Devi ◽  
Maham Habib ◽  
...  
Keyword(s):  
Hot Spring ◽  
Thermophilic Bacterium ◽  
Moderately Thermophilic

Anoxybacillus sp. AH1, an α-amylase-producing thermophilic bacterium isolated from Dargeçit hot spring

Biologia ◽  
2015 ◽  
Vol 70 (7) ◽  
Author(s):  
Ömer Acer ◽  
Hemşe Pırınççiğlu ◽  
Fatma Matpan Bekler ◽  
Reyhan Gül-Güven
Keyword(s):  
Hot Springs ◽  
Hot Spring ◽  
Thermophilic Bacteria ◽  
Potato Starch ◽  
Nitrogen Sources ◽  
Soluble Starch ◽  
Thermophilic Bacterium ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Optimum Growth Temperature

AbstractThe present study was conducted to isolate α-amylase-producing thermophilic bacteria from Darge¸cit hot springs in Turkey. The morphological, biochemical and physiological characterisation, as well as genetic analysis by 16S rRNA sequences indicated that the isolated strain AH1 was a member of Anoxybacillus genus. The strain was aerobe, Gram-positive and spore-forming rod, exhibiting optimum growth temperature and pH of 60ºC and 7.0-7.5, respectively. Optimization of growth medium and enzyme assay conditions for extracellular α-amylase production by the novel thermophilic Anoxybacillus sp. AH1 were carried out in many different media containing a variety of carbon and nitrogen sources. Among various carbon and nitrogen sources, peptone (2054.1 U/mL) at 1% and maltose (1862.9 U/mL) at 0.5% increased α-amylase activity, compared to controls. Moreover, a high enzyme production was observed with potato starch at 0.5% and 1% (2668.4 U/mL and 3627 U/mL, respectively), as well as with 1% soluble starch (2051.9 U/mL). The enzyme activity was found to be rather high in the presence of CaCl

scholarly journals Laceyella thermophila sp. nov., a thermophilic bacterium isolated from a hot spring

2017 ◽  
Vol 67 (8) ◽  
pp. 2953-2958 ◽  
Author(s):  
Hong Ming ◽  
Wei-Li Ji ◽  
Shuai Li ◽  
Zhuo-Li Zhao ◽  
Ling-Yu Zhang ◽  
...  
Keyword(s):  
Hot Spring ◽  
Thermophilic Bacterium
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