scholarly journals A novel genotype-independent technique for successful induction of somatic embryogenesis of adult plants of Jatropha curcas L. using petiole transverse Thin Cell Layer (TCL)

2021 ◽  
Vol 20 (2) ◽  
pp. 85-91
Author(s):  
Nicolás Mendoza-Peña Cristian ◽  
Kathrine (Trine) Hvoslef-Eide Anne
Keyword(s):  
Somatic Embryogenesis ◽  
Jatropha Curcas ◽  
Cell Layer ◽  
Thin Cell Layer ◽  
Jatropha Curcas L ◽  
Transverse Thin Cell Layer ◽  
Successful Induction ◽  
Independent Technique ◽  
Novel Genotype ◽  
Adult Plants

Thin Cell Layer Technology in Micropropagation of Jatropha curcas L.

2013 ◽  
pp. 33-42
Author(s):  
Duong Tan Nhut ◽  
Do Dang Giap ◽  
Bui Van The Vinh ◽  
Nguyen Thi Kim Loan ◽  
Nguyen Phuc Huy ◽  
...  
Keyword(s):  
Jatropha Curcas ◽  
Cell Layer ◽  
Thin Cell Layer ◽  
Jatropha Curcas L

scholarly journals Effects of amino acids and spermidine on somatic embryogenesis of Jatropha curcas L.

2014 ◽  
Vol 35 (3se) ◽  
Author(s):  
Do Dang Giap ◽  
Nguyen Thi Kim Loan ◽  
Tran Trong Tuan ◽  
Le Thanh Tuan ◽  
Huynh Le Thien Tu ◽  
...  
Keyword(s):  
Amino Acids ◽  
Somatic Embryogenesis ◽  
Jatropha Curcas ◽  
Jatropha Curcas L

scholarly journals Silver nanoparticles enhanced efficiency of explant surface disinfection and somatic embryogenesis in Begonia tuberous via thin cell layer culture

2021 ◽  
Vol 19 (2) ◽  
pp. 337-347
Author(s):  
Hoang Thanh Tung ◽  
Hoang Thi Van ◽  
Huynh Gia Bao ◽  
Le The Bien ◽  
Hoang Dac Khai ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Somatic Embryogenesis ◽  
Cell Layer ◽  
Initial Study ◽  
Thin Cell Layer ◽  
Flower Stalk ◽  
Time Treatment ◽  
Induction Rate ◽  
Thin Cell Layer Culture

In vitro culture establishment is one of the most important stages in micropropagation. The disinfectant effectiveness depends on the type of surface disinfectant, concentration and the time treatment. In this initial study, silver nanoparticles (AgNPs) were used as a disinfectant for petioles, flower stalks and stems of Begonia tuberous. In addition, thin cell layer culture (TCL) technique has been applied for the purpose of somatic embryogenesis. The results showed that AgNPs were effective in eliminating infectious microorganisms on B. tuberous explants; which were identified included 4 species of fungi (Fusarium sp., Aspergillus aculeatus, Trichoderma sp. and Penicillium sp.) and 1 species of bacteria (Pseudomonas sp.). At concentrations of 200 ppm and 300 ppm, AgNPs were not only effective in disinfection but also increased the induction rate of somatic embryogenesis in flower stalk TCL explants (approximately 40.00%); a similar effect was observed in stem TCL explants at the same concentration. Meanwhile, for petiole TCL explants, the induction rate of somatic embryogenesis was optimal when using AgNPs at a concentration of 100 - 300 ppm to disinfected the explant. In contrast, at high (400 ppm) or low (50 ppm) concentrations of AgNPs did not play a disinfecting role and stimulated somatic embryogenesis. In addition, explants derived from AgNPs sterilization did not show any abnormalities in somatic embryogenesis with shapes such as globular, heart, torpedo, and cotyledon. AgNPs showed double efficacy in sterilization of explants and improved efficiency of somatic embryogenesis from TCL petioles, flower stalks and stems explants; thus increasing the efficiency micropropagation of B. tuberous.

scholarly journals High Frequency Plant Regeneration System from Transverse Thin Cell Layer Section of In vitro Derived ‘Nadia’ Ginger Microrhizome

2014 ◽  
Vol 6 (1) ◽  
pp. 85-91
Author(s):  
Dikash Singh THINGBAIJAM ◽  
Devi Sunitibala HUIDROM
Keyword(s):  
High Frequency ◽  
Somatic Embryos ◽  
Cell Layer ◽  
Sucrose Concentration ◽  
Dichlorophenoxyacetic Acid ◽  
Thin Cell Layer ◽  
Regeneration System ◽  
Transverse Thin Cell Layer ◽  
2,4 Dichlorophenoxyacetic Acid

An efficient and reproducible procedure is outlined for rapid in vitro multiplication of Zingiber officinale var. ‘Nadia’ through high frequency shoot proliferation from transverse thin cell layer (tTCL) sections of in vitro derived microrhizome. In vitro derived microrhizome of size 500 μm in thickness was used as initial explants for induction of somatic embryos. Among the different phytohormones tested, tTCL explants shows maximum calli proliferation in medium containing 2 mg/L 2,4-Dichlorophenoxyacetic acid (88.30±0.11%). Reduced concentration of 2,4 Dichlorophenoxyacetic acid was supplemented with different cytokinins for regeneration of callus. Among the different medium tested, optimum redifferentiation of somatic embryos were observed in medium containing 0.2 mg/L 2,4 Dichlorophenoxyacetic acid and 6.0 mg/L BAP (141.08±0.25). Clump of regenerated plantlets were further subculture and transfer into microrhizome inducing medium containing high sucrose concentration (8%). Plantlets with well developed microrhizome were successfully acclimatized and eventually transferred to the field. The application of studying embryo section for regeneration of plants might be useful alternative to ginger improvement programme. Histological analysis showed formation of somatic embryos and regenerated adventitious shoot.

Effect of genotype, explant size, position, and culture medium on shoot generation of Gerbera jamesonii by receptacle transverse thin cell layer culture

2007 ◽  
Vol 111 (2) ◽  
pp. 146-151 ◽  
Author(s):  
Duong Tan Nhut ◽  
Truong Thi Thuy An ◽  
Nguyen Thi Dieu Huong ◽  
Nguyen Trinh Don ◽  
Nguyen Thanh Hai ◽  
...  
Keyword(s):  
Culture Medium ◽  
Cell Layer ◽  
Thin Cell Layer ◽  
Gerbera Jamesonii ◽  
Transverse Thin Cell Layer ◽  
Thin Cell Layer Culture
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