scholarly journals In vitro regeneration protocol through direct organogenesis for Jatropha curcas L. (Euphorbiaceae) accessions in Ethiopia

2019 ◽  
Vol 18 (31) ◽  
pp. 991-1003 ◽  
Author(s):  
Fufa Hundessa ◽  
Tesema Meseret ◽  
Daksa Jiregna
Keyword(s):  
Jatropha Curcas ◽  
In Vitro Regeneration ◽  
Direct Organogenesis ◽  
Jatropha Curcas L

scholarly journals In vitro regeneration from different ages of petioles of physic nut (Jatropha curcas L.)

2014 ◽  
Vol 13 (2) ◽  
pp. 265-273 ◽  
Author(s):  
Mahalakshmi Ravi ◽  
Eganathan Palanisami ◽  
Parida Ajay
Keyword(s):  
Jatropha Curcas ◽  
In Vitro Regeneration ◽  
Physic Nut ◽  
Jatropha Curcas L ◽  
Different Ages

scholarly journals In vitro shoot multiplication of the biodiesel plant Jatropha curcas L. through direct organogenesis

2014 ◽  
Vol 49 (1) ◽  
pp. 41-46 ◽  
Author(s):  
MAKMS Hassan ◽  
N Begum ◽  
LS Bari ◽  
MAA Jahan
Keyword(s):  
Jatropha Curcas ◽  
Basal Medium ◽  
Shoot Multiplication ◽  
Nodal Explants ◽  
Direct Organogenesis ◽  
Shoot Induction ◽  
Jatropha Curcas L ◽  
Half Strength ◽  
Regenerated Plantlets

An efficient protocol was established for in vitro shoot multiplication of the biodiesel plant, Jatropha curcas L. (Euphorbiaceae) through direct organogenesis using shoot tip and nodal explants. Best shoot induction was observed on MS basal medium supplemented with 1.5 mg/l BAP + 0.5 mg/l NAA, in which 86.2% of nodal explants responded to produce maximum number (7.2 ± 0.68) of shoots per culture. In vitro raised shoots rooted on half strength MS medium with 1.0 mg/l IAA. The survival rate of regenerated plantlets was 85%. DOI: http://dx.doi.org/10.3329/bjsir.v49i1.18854 Bangladesh J. Sci. Ind. Res. 49(1), 41-46, 2014

scholarly journals Methods to assess the viability of cryopreserved Jatropha curcas L. seed germplasm

2016 ◽  
Vol 18 (2) ◽  
pp. 391-398 ◽  
Author(s):  
A.N. SALOMÃO ◽  
I.R.I. SANTOS ◽  
S.C.B.R. JOSÉ ◽  
J.P. DA SILVA ◽  
B.G. LAVIOLA
Keyword(s):  
Jatropha Curcas ◽  
Germination Percentage ◽  
Experimental Sample ◽  
Embryonic Axes ◽  
Medicinal Uses ◽  
Jatropha Curcas L ◽  
Significant Difference ◽  
Before And After ◽  
Potential Applications

ABSTRACT Jatropha curcas L. is a plant species with many potential applications, especially medicinal uses (hypoglycemic, anti-inflammatory, haemostatic, healing, anti-tumor). The objective of this study was to test germination in moist paper rolls for whole seeds and in vitro for excised embryonic axes, in an attempt to identify the best method to assess the quality of J. curcas seed germplasm, cryopreserved with different water contents. The experimental sample with a 6.2% moisture content (MC) was divided in subsamples which were hydrated and dehydrated for 0 (control), 4, 8, 11 and 24h. The initial germination percentages were 63% for whole seeds and 81% for excised embryonic axes. After exposure to liquid nitrogen (LN), germination percentages were 48% (whole seeds) and 57% (excised embryonic axes). There was no significant difference between germination percentages in embryonic excised from seeds subjected or not subjected to freezing, with different MC. In contrast, there was a reduction of the whole seed germination percentage when exposed to LN (contrast = 0.17, standard error = 0.04, t = 4.09, p = 0.001) and not for the hydration and dehydration treatments. The methodology based on in vitro cultures of the embryonic axis isolated from seeds stored in LN with distinct MC values was more efficient than the standard germination test to evaluate the viability of J. curcas seeds before and after LN storage.

scholarly journals Induction of different types of callus in Jatropha curcas L. hybrid accession at in vitro condition

2017 ◽  
pp. 874-879
Author(s):  
Alexandre Bosco de Oliveira ◽  
◽  
Wagner A. Vendrame ◽  
Luciana Cardoso Nogueira Londe ◽  
Massy Sanaey ◽  
...  
Keyword(s):  
Jatropha Curcas ◽  
Jatropha Curcas L ◽  
Different Types

In vitro regeneration from petiole explants of non-toxic Jatropha curcas

2011 ◽  
Vol 33 (1) ◽  
pp. 146-151 ◽  
Author(s):  
Nitish Kumar ◽  
K.G. Vijay Anand ◽  
Muppala P. Reddy
Keyword(s):  
Jatropha Curcas ◽  
In Vitro Regeneration ◽  
Petiole Explants

scholarly journals In-vitro Regeneration from Direct and Indirect Organogenesis of Crescentia alata Kunth an Important Multipurpose Medicinal Tree

2021 ◽  
pp. 48-58
Author(s):  
R. Abinaya
Keyword(s):  
Shoot Organogenesis ◽  
In Vitro Regeneration ◽  
Basal Medium ◽  
Direct Organogenesis ◽  
Ms Medium ◽  
Indirect Organogenesis ◽  
Medicinal Tree ◽  
Short Period ◽  
In Vitro Clonal Propagation

In this present work, an in-vitro regeneration protocol for Crescentia alata (C. alata) was developed using various explants on Murashige and Skoog (MS) medium augmented with different concentrations and combinations of plant growth regulators (PGRs) for direct and indirect regeneration. The direct organogenesis was established from nodes and internodes on MS medium supplemented with cytokinins and auxins. The indirect organogenesis via callus phase was obtained from leaf, nodes and internodes on MS medium supplemented with different concentrations of PGRs. The high frequency shoot organogenesis were achieved directly from nodal explants were cultured on MS medium supplemented with 3.0 mg/L BAP+0.5 mg/L KIN +1.0 mg/L NAA. Indirect organogenesis callogenic frequency was optimized at the concentration of MS medium containing 1.0 mg/L BAP + 5.0 mg/L IAA. The callus was obtained from all the explants were used, among these explants internodal explants gave best result on MS medium supplemented with different concentrations of cytokinins and auxins for indirect organogenesis experiment. Indirect organogenesis the highest number of shoot regeneration was obtained in MS Basal Medium with 4.0 mg/L BAP + 0.5 mg/L KIN + 2.0 mg/L NAA from internodal explants. For root formation the regenerative shoots which were sub cultured on MS medium containing different ratios of auxins. The rooted plantlets were transferred successfully to the pots containing sterilized soil and were successfully hardened at greenhouse condition for 20 days then exposed to the natural environment. This is the first successful micropropagation report of an efficient and rapid in-vitro clonal propagation protocol for C. alata by direct and indirect shoot organogenesis through various explants, which can be employed for conservation of this important medicinal tree species as well as the utilization of an biologically important active biomolecules. This protocol can be very useful to obtain plants from various explants, without the requirement of meristematic regions, enabling the obtainment of a higher number of plants in short period.

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