scholarly journals Antioxidant, cytotoxic, and antidiabetic activities of Dendropanax morbifera extract for production of health-oriented food materials

2019 ◽  
Vol 18 (6) ◽  
pp. 124-129
Author(s):  
Lee KiBeom ◽  
Kim Andy
Keyword(s):  
Dendropanax Morbifera ◽  
Dendropanax Morbifera Extract

Novel Co-fermentation of Dendropanax morbifera Extract to Produce γ-aminobutyric Acid and Poly-γ-glutamic Acid

2019 ◽  
Vol 25 (6) ◽  
pp. 785-791
Author(s):  
Woong-Kyu Yoon ◽  
Jae-Won Choi ◽  
Jong-Soon Lim ◽  
Coralia V. Garcia ◽  
Sam-Pin Lee
Keyword(s):  
Glutamic Acid ◽  
Aminobutyric Acid ◽  
Dendropanax Morbifera

Anti-obesity Effect of Gold Nanoparticles from Dendropanax morbifera Léveille by Suppression of Triglyceride Synthesis and Downregulation of PPARγ and CEBPα Signaling Pathways in 3T3-L1 Mature Adipocytes and HepG2 Cells

2020 ◽  
Vol 16 (2) ◽  
pp. 196-203 ◽  
Author(s):  
Myoung Hi Yi ◽  
Shakina Yesmin Simu ◽  
Sungeun Ahn ◽  
Verónica Castro Aceituno ◽  
Chao Wang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Fatty Liver ◽  
Lipid Accumulation ◽  
Hepg2 Cells ◽  
Cellular Level ◽  
Dendropanax Morbifera ◽  
Immunoblotting Assay ◽  
Triglyceride Content ◽  
20 Nm ◽  
Proliferation Assays

Background: Biosynthesis of gold nanoparticles from medicinal plants has become an interesting strategy in biomedical research due to its exclusive properties including less toxic cellular level through its ecofriendly biological function. Objective: To examine the anti-lipid accumulation effect of spherical gold nanoparticles (size 10-20 nm) synthesized from Dendropanax morbifera Léveille (D-AuNPs) in both 3T3-L1 and HepG2 cells. Method: 3T3-L1 preadipocytes and HepG2 hepatocytes were stimulated with cocktail media to generate obese and fatty liver disease models. Cell cytotoxicity and cell proliferation assays were performed in adipocytes at different stages of growth. An anti-lipid accumulation assay was performed in 3T3-L1 obese and HepG2 fatty liver models using different doses of D-AuNPs. Expression of adipogenic genes of PPARγ, CEBPα, Jak2, STAT3, and ap2 and hepatogenic genes PPARα, FAS, and ACC was measured by real-time PCR. In addition, protein expression of PPARγ and CEBPα was evaluated by immunoblotting assay. Result: We found that D-AuNPs (size 10–20 nm) at concentrations up to 100 µg/ml were nontoxic to 3T3-L1 and HepG2 at post-confluent and mature stages. In addition, pretreatment of D-AuNPs at post-confluent stage reduced triglyceride content. In addition, the adipogenesis process was negatively controlled by D-AuNPs, with downregulated PPARγ, CEBPα, Jak2, STAT3, and ap2 expression in 3T3-L1 cells and FAS and ACC levels in HepG2 cells. Conclusion: These data indicated that D-AuNPs exert antiadipogenic properties. We hypothesize that Dendropanax contains a large amount of phenolic compound that coats the surface of gold nanoparticles and has the ability to reduce the excess amount of lipid in both cell lines.

Rutin from Dendropanax morbifera Leveille Protects Human Dopaminergic Cells Against Rotenone Induced Cell Injury Through Inhibiting JNK and p38 MAPK Signaling

2014 ◽  
Vol 39 (4) ◽  
pp. 707-718 ◽  
Author(s):  
Se-Eun Park ◽  
Kumar Sapkota ◽  
Jun-Hui Choi ◽  
Myung-Kon Kim ◽  
Young Hoi Kim ◽  
...  
Keyword(s):  
P38 Mapk ◽  
Cell Injury ◽  
Mapk Signaling ◽  
Dopaminergic Cells ◽  
Dendropanax Morbifera

scholarly journals Synergistic Protection by Isoquercitrin and Quercetin against Glutamate-Induced Oxidative Cell Death in HT22 Cells via Activating Nrf2 and HO-1 Signaling Pathway: Neuroprotective Principles and Mechanisms of Dendropanax morbifera Leaves

Antioxidants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 554
Author(s):  
Hye-Jin Park ◽  
Ha-Neul Kim ◽  
Chul Young Kim ◽  
Min-Duk Seo ◽  
Seung-Hoon Baek
Keyword(s):  
Cell Death ◽  
Nuclear Translocation ◽  
Interaction Analysis ◽  
Cell Model ◽  
Antioxidant Compounds ◽  
Heme Oxygenase 1 ◽  
Related Factor ◽  
Protective Effects ◽  
Ht22 Cells ◽  
Dendropanax Morbifera

Dendropanax morbifera leaves (DML) have long been used as traditional medicine to treat diverse symptoms in Korea. Ethyl acetate-soluble extracts of DML (DMLE) rescued HT22 mouse hippocampal neuronal cells from glutamate (Glu)-induced oxidative cell death; however, the protective compounds and mechanisms remain unknown. Here, we aimed to identify the neuroprotective ingredients and mechanisms of DMLE in the Glu-HT22 cell model. Five antioxidant compounds were isolated from DMLE and characterized as chlorogenic acid, hyperoside, isoquercitrin, quercetin, and rutin by spectroscopic methods. Isoquercitrin and quercetin significantly inhibited Glu-induced oxidative cell death by restoring intracellular reactive oxygen species (ROS) levels and mitochondrial superoxide generation, Ca2+ dysregulation, mitochondrial dysfunction, and nuclear translocation of apoptosis-inducing factor. These two compounds significantly increased the expression levels of nuclear factor erythroid-2-related factor 2 (Nrf2) and heme oxygenase 1 (HO-1) in the presence or absence of Glu treatment. Combinatorial treatment of the five compounds based on the equivalent concentrations in DMLE showed that significant protection was found only in the cells cotreated with isoquercitrin and quercetin, both of whom showed prominent synergism, as assessed by drug–drug interaction analysis. These findings suggest that isoquercitrin and quercetin are the active principles representing the protective effects of DMLE, and these effects were mediated by the Nrf2/HO-1 pathway.

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