scholarly journals Development and validation of analytical methodology for quantification of total flavonoids of Morus nigra by ultraviolet-visible absorption spectrophotometry

2018 ◽  
Vol 17 (23) ◽  
pp. 724-729
Author(s):  
Alves Sampaio Pedrita ◽  
Andreysa Pereira Teixeira Hyany ◽  
Andrezza Carvalho de Souza Nathalia ◽  
Marcos Teixeira de Alencar-Filho Jose ◽  
Rocha Souza Grasielly ◽  
...  
Keyword(s):  
Total Flavonoids ◽  
Visible Absorption ◽  
Analytical Methodology ◽  
Absorption Spectrophotometry ◽  
Morus Nigra ◽  
Development And Validation

scholarly journals Development and validation of an analytical method for quantification of total flavonoids in Alternanthera brasiliana by ultraviolet-visible absorption spectrophotometry

2019 ◽  
Vol 18 (30) ◽  
pp. 920-927 ◽  
Author(s):  
M. T. de Alencar Filho José ◽  
A. P. Teixeira Hyany ◽  
S. de Carvalho Iure ◽  
V. V. de O. Alencar Milenna ◽  
A. C. de Souza Nathália ◽  
...  
Keyword(s):  
Analytical Method ◽  
Total Flavonoids ◽  
Visible Absorption ◽  
Absorption Spectrophotometry ◽  
Development And Validation

scholarly journals Development and validation of analytical method by spectrophotometry UV-Vis for quantification of flavonoids in leaves of Senna occidentalis Link

2022 ◽  
Vol 11 (1) ◽  
pp. e14411118584
Author(s):  
Vanessa da Silva Luna ◽  
Karina Perrelli Randau ◽  
Magda Rhayanny Assunção Ferreira ◽  
Luiz Alberto Lira Soares
Keyword(s):  
Reaction Time ◽  
Aluminum Chloride ◽  
Analytical Method ◽  
Folk Medicine ◽  
Solvent Ratio ◽  
Total Flavonoids ◽  
Analytical Methodology ◽  
Processed Products ◽  
Development And Validation ◽  
Validation Of Analytical Method

Senna occidentalis Link (Fabaceae), known as “fedegoso”, is used in folk medicine due to its anti-inflammatory, antioxidant, muscle relaxant and inhibiting lipid peroxidation imputed to flavonoids contained in its composition. The species is also a constituent of processed products are commercialized in various parts of the world including Brazil, although there are few reports in the literature about the development of an analytical method for quantification of flavonoids in it. The main purpose of this research was the evaluation of an analytical methodology to determinate the content of total flavonoids in leaves of S. occidentalis, for quality control. The parameters evaluated were: drug: solvent ratio, concentration of aluminum chloride and reaction time. The quantification of total flavonoids was obtained after reaction with aluminum chloride by UV/Vis spectrophotometry. The results revealed a maximum absorption peak (λ = 392 nm) was the amount of 0.5 g of a plant raw in 100 mL of solvent and there were no significant influences between the concentrations of aluminum chloride or hydroalcoholic solutions analyzed. The evaluation of reaction time allowed to characterize the kinetics as slow, stabilizing from 60 min, choosing this as reading time. The method was specific, linear, precise, accurate and robust, according to the specifications set in RDC n. 166/2017. Finally, the results of the study showed that the measured methodology is simple and accurate and can be applied for quality assessment of the species S. occidentalis.

Development and validation of an analytical methodology for the determination of p,p′-DDT, p,p′-DDE and p,p′-DDD in fish oil pills

2007 ◽  
Vol 86 (2) ◽  
pp. 183-188 ◽  
Author(s):  
Juan José Berzas Nevado ◽  
Rosa Carmen Rodríguez Martín-Doimeadiós ◽  
Francisco Javier Guzmán Bernardo ◽  
Nuria Rodríguez Fariñas
Keyword(s):  
Fish Oil ◽  
Analytical Methodology ◽  
Development And Validation

Analytical Method Development and Validation of Glipizide to Determine Residual solvents by head Space-Gas Chromatography

2021 ◽  
pp. 2440-2444
Author(s):  
Sanapala Srinivasa Rao ◽  
A. Vijayalakshmi
Keyword(s):  
Gas Chromatography ◽  
Method Development ◽  
Limit Of Detection ◽  
Dimethyl Formamide ◽  
Analytical Methodology ◽  
Residual Solvents ◽  
Flame Ionization ◽  
Ich Guidelines ◽  
Method Development And Validation ◽  
Development And Validation

Residual solvents in Pharmaceuticals are termed as organic volatile impurities. These are the chemicals that are used in the manufacture of drug substance or excipients or use in the preparation of final formulation. Most of the available methods use liquid chromatography which could be expensive and time consuming. Hence, an analytical methodology was developed for the quantification of residual solvents in Glipizide using a headspace gas chromatography (HSGC) with the help of flame ionization detector (FID). Methanol, acetone and dimethyl formamide as residual solvents were determined in Glipizide. Analysis was performed by headspace GC/FID method on Auto system- HS40. Nitrogen was used as a carrier gas and the separation of residual solvents was achieved by DB-Wax 0.25mm, 0.3mcm column. The thermostat temperature was 115 °C for 40 minutes for each vial. % RSD for nine injections obtained are in acceptance criteria. The correlation coefficient R2 obtained greater than 0.99. The method parameters were validated includes specificity, limit of detection and quantification, accuracy, linearity, precision, and robustness. According to the International Conference on Harmonization (ICH) guidelines, a new simple, specific, accurate and precise method was developed and validated.

scholarly journals Development and validation of a high performance liquid chromatography-diode array detection (HPLC-DAD) method for the quantification of rutin and isoquercetin in Morus nigra L. (Moraceae)

2018 ◽  
Vol 17 (34) ◽  
pp. 1048-1056 ◽  
Author(s):  
Alves Sampaio Pedrita ◽  
Rocha Souza Grasielly ◽  
Guilherme Sousa de Sá Pedro ◽  
Andrezza Carvalho de Souza Nathália ◽  
Marcos Teixeira de Alencar Filho José ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Diode Array ◽  
Diode Array Detection ◽  
Morus Nigra ◽  
Array Detection ◽  
Development And Validation

Oral sustained release nystatin tablets for the treatment of oral candidiasis: formulation development and validation of UV spectrophotometric analytical methodology for content determination

2009 ◽  
Vol 36 (5) ◽  
pp. 594-600 ◽  
Author(s):  
Michelle Maria Gonçalves Barão de Aguiar ◽  
Raphael Pereira de Albuquerque ◽  
Daniel Savignon Marinho ◽  
Bárbara Regina Souza Braga ◽  
Camila Braga Dornelas ◽  
...  
Keyword(s):  
Sustained Release ◽  
Oral Candidiasis ◽  
Formulation Development ◽  
Analytical Methodology ◽  
Content Determination ◽  
Development And Validation
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