scholarly journals The antibacterial activity of bacterial endophytes isolated from Combretum molle

2018 ◽  
Vol 17 (8) ◽  
pp. 255-262 ◽  
Author(s):  
O. Diale Mamonokane ◽  
Ubomba-Jaswa Eunice ◽  
H. Serepa-Dlamini Mahloro
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Endophytes ◽  
Combretum Molle

scholarly journals BIOPROSPECTING FOR BACTERIAL ENDOPHYTES ASSOCIATED WITH ZINGIBERACEAE FAMILY RHIZOMES IN SIBOLANGIT FOREST, NORTH SUMATERA

2020 ◽  
Vol 1 (1) ◽  
pp. 27-36
Author(s):  
Jendri Mamangkey
Keyword(s):  
Antibacterial Activity ◽  
Protease Activity ◽  
Antibacterial Agent ◽  
Phosphate Solubilization ◽  
Antibacterial Activities ◽  
Chemical Diversity ◽  
Bacterial Endophytes ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Paper Disc

The present study was conducted aiming to isolate and characterize endophytic bacterial isolates with antibacterial ability, phosphate solubilization, and proteolytic activity from rhizomes of the Zingiberaceae family (Etlingera sp., Globba patens, Globba pendula, and Zingiber multibracteata). Nineteen bacterial isolates were obtained from Zingiberaceae rhizomes with isolate codes of EZS27, EZS18, EZS19, EZS25, EZS16, EZS08, EZS09, EZS13, EZS20, EZS14, EZS10, EZS11, EZS03, EZS05, EZS06, EZS43, EZS45, EZS47, and EZS28. The screening of the endophytes for antibacterial activity was done through the paper disc method. Four bacterial isolates presented antibacterial activities. EZS06 isolate inhibited the growth of EPEC (11 mm), P. vulgaris ATCC 13315 (10 mm), and L. monocytogenes BTCC B693 (9 mm). Also, EZS20 isolate inhibited the growth of S. aureus ATCC 29213 (17 mm), EZS28 isolate inhibited MRSA ATCC 43300 (8.6 mm), and EZS45 isolate inhibited S.  Epidermidis ATCC 12228 (9 mm). The EZS19, EZS03, and EZS16 isolates dissolved the phosphate most effectively. Eight isolates (EZS19, EZS47, EZS27, EZS25, EZS09, EZS20, EZS45, and EZS06) showed the best protease activity. In general, our results showed that the endophytic bacterial strains can be used as a new and useful antibacterial agent since it showed antibacterial activity and chemical diversity. Furthermore, it also has the potential for exploitation in a wide variety of medical, agricultural, and industrial areas.

scholarly journals Antibacterial Activities of Crude Secondary Metabolite Extracts from Pantoea Species Obtained from the Stem of Solanum mauritianum and Their Effects on Two Cancer Cell Lines

2019 ◽  
Vol 16 (4) ◽  
pp. 602 ◽  
Author(s):  
Nkemdinma Uche-Okereafor ◽  
Tendani Sebola ◽  
Kudzanai Tapfuma ◽  
Lukhanyo Mekuto ◽  
Ezekiel Green ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Soil Conditions ◽  
Bacterial Endophytes ◽  
Crude Extracts ◽  
Solanum Mauritianum

Endophytes are microorganisms that are perceived as non-pathogenic symbionts found inside plants since they cause no symptoms of disease on the host plant. Soil conditions and geography among other factors contribute to the type(s) of endophytes isolated from plants. Our research interest is the antibacterial activity of secondary metabolite crude extracts from the medicinal plant Solanum mauritianum and its bacterial endophytes. Fresh, healthy stems of S. mauritianum were collected, washed, surface sterilized, macerated in PBS, inoculated in the nutrient agar plates, and incubated for 5 days at 30 °C. Amplification and sequencing of the 16S rRNA gene was applied to identify the isolated bacterial endophytes. These endophytes were then grown in nutrient broth for 7–14 days, after which sterilized Amberlite® XAD7HP 20–60 mesh (Merck KGaA, Darmstadt, Germany) resin was added to each culture to adsorb the secondary metabolites, which were later extracted using ethyl acetate. Concentrated crude extracts from each bacterial endophyte were tested for antibacterial activity against 11 pathogenic bacteria and two human cancer cell lines. In this study, a total of three bacterial endophytes of the Pantoea genus were identified from the stem of S. mauritianum. The antibacterial test showed that crude secondary metabolites of the endophytes and stem of S. mauritianum possessed antibacterial properties against pathogenic microbes such as Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa, with concentrations showing inhibition ranging from 0.0625 to 8.0000 mg/mL. The anticancer analysis showed an increase in cell proliferation when A549 lung carcinoma and UMG87 glioblastoma cell lines were treated with both the plant and endophytes’ crude extracts. As far as we know, this is the first study of its kind on Solanum mauritianum in South Africa showing S. mauritianum endophytes having activity against some of the common human pathogenic organisms.

Antibacterial activity of herbal preparation to treat sore throats

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
J Reichling ◽  
U Suschke ◽  
A Suter
Keyword(s):  
Antibacterial Activity ◽  
Herbal Preparation

Antibacterial activity of Celastrol against Bacillus subtilis

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
N Padilla-Montaño ◽  
IL Bazzocchi ◽  
L Moujir
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis

Antibacterial Activity of Rhinacanthin Rich Rhinacanthus nasutus Extract

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
P Panichayupakaranant ◽  
P Puttarak
Keyword(s):  
Antibacterial Activity ◽  
Rhinacanthus Nasutus

Antibacterial activity comparison of several Portuguese Thymus essential oils

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
SA Dandlen ◽  
AC Figueiredo ◽  
LG Pedro ◽  
JG Barroso ◽  
MG Miguel ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Activity Comparison
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