scholarly journals Molecular genetic diversity in a core of cocoa (Theobroma cacao L.) clones with potential for selection of disease resistance, plant height and fruit production

2016 ◽  
Vol 15 (44) ◽  
pp. 2517-2523
Author(s):  
Batista Lins Adeilson ◽  
Ramos Lawinscky Pabliane ◽  
Marlene Magalhaes Barbosa Antonia ◽  
Amato Gaiotto Fernanda ◽  
Xavier Correa Ronan
Keyword(s):  
Genetic Diversity ◽  
Disease Resistance ◽  
Plant Height ◽  
Theobroma Cacao ◽  
Molecular Genetic ◽  
Fruit Production ◽  
Molecular Genetic Diversity ◽  
Theobroma Cacao L ◽  
Selection Of

scholarly journals Molecular genetic diversity of the Turkish national hazelnut collection and selection of a core set

2017 ◽  
Vol 13 (5) ◽  
Author(s):  
Süleyman Can Öztürk ◽  
Hüseyin İrfan Balık ◽  
Selda Kayalak Balık ◽  
Gökhan Kızılcı ◽  
Ömür Duyar ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Genetic ◽  
Molecular Genetic Diversity ◽  
Core Set ◽  
Selection Of

Genetic diversity of native cultivated cacao accessions (Theobroma cacao L.) in Nicaragua

2012 ◽  
Vol 10 (3) ◽  
pp. 254-257
Author(s):  
Erwin Aragon ◽  
Claudia Rivera ◽  
Helena Korpelainen ◽  
Aldo Rojas ◽  
Paula Elomaa ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Theobroma Cacao ◽  
Phytophthora Palmivora ◽  
Genetic Composition ◽  
Breeding Programmes ◽  
Black Pod ◽  
Black Pod Disease ◽  
Parental Lines ◽  
Resistant Genotypes ◽  
Theobroma Cacao L

A total of 60 farmers' cacao accessions (Theobroma cacao L.) from Nicaragua were investigated using microsatellite markers to reveal their genetic composition and to identify potentially resistant genotypes against the black pod disease caused by Phytophthora palmivora. These accessions were compared with 21 breeders' accessions maintained locally, two Criollo accessions from Costa Rica and two accessions from Ecuador. The analyses showed a low level of differentiation among groups of farmers' accessions (FST = 0.06) and that six Nicaraguan accessions were genetically closely related to the two Criollo accessions used as a reference. In addition, seven distinct genotypes were found to have allelic composition that may indicate linkage to resistance alleles, thus being potential parental lines in future breeding programmes.

Association mapping of seed and disease resistance traits in Theobroma cacao L.

Planta ◽  
2016 ◽  
Vol 244 (6) ◽  
pp. 1265-1276 ◽  
Author(s):  
Lambert A. Motilal ◽  
Dapeng Zhang ◽  
Sue Mischke ◽  
Lyndel W. Meinhardt ◽  
Michel Boccara ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Association Mapping ◽  
Theobroma Cacao ◽  
Theobroma Cacao L ◽  
Resistance Traits

scholarly journals GENETIC DIVERGENCE AND MORPHO-AGRONOMIC PERFORMANCE OF JATROPHA CURCAS L. CLONES FOR SELECTION OF CLONAL VARIETIES

2016 ◽  
Vol 29 (4) ◽  
pp. 841-849
Author(s):  
ADRIANA QUEIROZ DE ALMEIDA ◽  
SIMONE ALVES SILVA ◽  
VANESSA DE OLIVEIRA ALMEIDA ◽  
DEOCLIDES RICARDO DE SOUZA ◽  
GILMARA DE MELO ARAÚJO
Keyword(s):  
Genetic Diversity ◽  
Multivariate Analysis ◽  
Plant Height ◽  
Genetic Divergence ◽  
Morphological Characterization ◽  
Stem Diameter ◽  
Agronomic Performance ◽  
And Performance ◽  
Lower Contribution ◽  
Selection Of

ABSTRACT The knowledge about genetic diversity of jatropha crop is important for genetic conservation resources and breeding of this species. The aim of this study was to evaluate the genetic diversity and performance of jatropha clones through morphological characterization to selection of clonal varieties for biofuels production. The clones were obtained through shoot cuttings from previous selection in a population of half-sibs progenies. The morphoagronomic analyses of clones was carried out at 180 days after transplantation and were evaluated plant height, stem diameter, number of primary branches and number of secondary branches, number of bunches and number of fruits per plant. Evaluating clones performance, significant results were found for the number of secondary branches. About analysis of genetic diversity, the measures of dissimilarity genetic varied from 0.62 to 13.11, this way, the UFRBPR14 and UFRBPR15 clones were more divergent. The Tocher method was efficient to verify formation of four groups. The characteristics that most contributed to the divergence among clones were branches number, height and number of bunches, and, stem diameter had lower contribution. The jatropha clones differed only in the secondary branches number and multivariate analysis showed divergence among the jatropha clones with formation of four groups. Also, branches number, plant height and number of bunches were characteristic that contributed to genetic divergence.

Study on molecular genetic diversity of native duck breeds in China

2006 ◽  
Vol 62 (04) ◽  
pp. 603-611
Author(s):  
H. LI ◽  
N. YANG ◽  
K. CHEN ◽  
G. CHEN ◽  
Q. TANG ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Genetic ◽  
Molecular Genetic Diversity

scholarly journals Efecto de la gallinaza en plantaciones de cacao (Theobroma cacao L.) en desarrollo.

2016 ◽  
Vol 8 (1) ◽  
pp. 81 ◽  
Author(s):  
María Orozco ◽  
Susanne Thienhaus
Keyword(s):  
Plant Height ◽  
Theobroma Cacao ◽  
Growth And Development ◽  
Mineral Fertilizer ◽  
Juvenile Stage ◽  
Chicken Manure ◽  
Mineral Fertilizers ◽  
Stalk Diameter ◽  
Theobroma Cacao L ◽  
Lower Production

We studied the effect of the organic chicken manure on the growth and development of cocoa trees (Theobroma cacao L.), during their juvenile stage. The experiment was initiated in February, 1993, in three commercial plantations, 20 months old, located in the county of El Rama, Region V. During the following 14 months we carried out three application , comparing, the three levels of chicken manure (454 , 908 . and 1,362 g per tree and application) with mineral fertilizer (three applications , of 15-15-15fertilizers100 g per tree, plus one application of, urea 100 g per tree), and a check without any applications. A random complete bolck design was utilized and means were compared by, Duncan’s test and regression and correlation analyses were also made. The results showed that with the application of 1,362 g of chicken manure, a significatively higher effect over the initial production of cocoa, and an increase of the stalk diameter were observed. The applications of 454 and 908, of chicken manure resulted in production levels equal to those of the treatment with mineral fertilizers. In relation to plant height, we did not find statistical differences among treatments. The treatment without fertilizer , showed the smallest growth and lower production.

scholarly journals ANALISIS KERAGAMAN GENETIK PLASMA NUTFAH KAKAO (Theobroma cacaoL.) BERDASARKANMARKA SSR / Analysis of Genetic Variability Germplasm of Cacao (Theobroma cacaoL.)Basedon SSR Marker

2020 ◽  
Vol 17 (4) ◽  
pp. 156
Author(s):  
Surti Kurniasih ◽  
Rubiyo Rubiyo ◽  
Asep Setiawan ◽  
Agus Purwantara ◽  
Sudarsono Sudarsono
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Ssr Markers ◽  
Theobroma Cacao ◽  
Simple Sequence Repeat ◽  
Ssr Marker ◽  
Gene Diversity ◽  
Sequence Repeat ◽  
Theobroma Cacao L ◽  
Simple Sequence

<p>Microsatellite or simple sequence repeat (SSR) markers have proven to be an excellent tool for cultivar identification, pedigree analysis, and genetic distance evaluations among organisms. The objectives of this research were to characterize cacao collection of Indonesian Coffee and Cacao Research Institute (ICCRI) and to analyze their genetic diversity using SSR markers. In this research, 39 SSR primer pairs were used to amplify genomic DNA of 29 cacao clones. Amplified SSR fragments for each primer pair were scored as individual band and used to determine genetic distance among evaluated cacao clones. Results of the experiment indicated that all SSR primer pairs evaluated were able to produce SSR markers for 29 cacao clones. The results also indicated that 34 out of 39 microsatellite loci evaluated were polymorphic, while 5 others were monomorphic. The total number of observed alleles among 29 clones was 132. Number of alleles per locus ranged from 4-8, with an average of 5.5 alelles per locus. Results of data analysis indicated that the PIC value was 0.665, the observed heterozigosity (Ho) was 0.651, and the gene diversity (He) was 0.720. The PIC, Ho, and He values were considered high. Genetic distances were evaluated using NTSys version 2.1 and dendrogram was constructed. Results of analysis indicated that 12 cacao clones evaluated were clustered in the first group with diversity coefficient of &lt; 3.75. Nine cacao clones were in the second group but with the same value of diversity coefficient (&lt;7.50). The rest of the cacao clones were in the third group with diversity coefficient of&gt;7.50. Based on those finding, all SSR primer pairs evaluated could be used to analyze cacao genome and be useful for genetic diversity analysis of cacao germplasm. The SSR marker analysis in ICCRI cacao collections resulted in high PIC, high observed heterozygosity, and high genetic diversity.</p><p>Key words: Theobroma cacao L, microsatelite, molecular marker, genetic diversity, heterozygosity</p><p> </p><p><strong>Abstrak</strong></p><p>Marka mikrosatelit atau sekuens sederhana berulang (simple sequence repeat = SSR) terbukti merupakan alat yang bagus untuk identifikasi kultivar, analisis pedigree, dan evaluasi jarak genetik berbagai organisme. Penelitian ini bertujuan untuk:1) karakterisasi kakao koleksi Pusat penelitian Kopi dan Kakao Indonesia menggunakan marka SSR dan 2) analisis keragaman genetik klon-klon kakao koleksi dengan menggunakan marka SSR. Dalam penelitian ini, 39 pasangan primer SSR telah digunakan untuk amplifikasi DNA genomik dari 29 klon kakao. Skoring pita SSR hasil amplifikasi menggunakan masing-masing pasangan primer dilakukan secara terpisah dan digunakan untuk menentukan jarak genetik di antara klon kakao yang dievaluasi. Hasil percobaan menunjukkan bahwa semua pasangan primer SSR yang digunakan mampu menghasilkan pita DNA hasil amplifikasi (marka SSR) untuk 29 klon kakao yang diuji. Hasil penelitian juga menunjukkan bahwa 34 dari 39 lokus SSR yang dianalisis bersifat polimorfik sedangkan lima primer yang lain bersifat monomorfik. Dari 29 klon kakao yang dievaluasi, telah berhasil diamplifikasi sebanyak 132 alel, dengan kisaran antara 4-8 alel/lokus. Rataan jumlah alel per lokus sebanyak 5,50. Hasil analisis data yang dilakukan juga menunjukkan nilai PIC untuk marka SSR yang digunakan sebesar 0,665. Untuk populasi klon kakao yang dievaluasi, diperoleh nilai rataan heterosigositas pengamatan (Ho) sebesar 0,651 dan rataan diversitas gen (He) sebesar 0,720. Nilai PIC Ho dan He yang didapat tergolong tinggi. Berdasarkan analisis keragaman dengan menggunakan program NTSys, diperoleh hasil 12 klon kakao berada dalam grup pertama (koefisien keragaman&lt;3,75) dan9 klon berada dalam grup kedua, dengan koefisien keragaman &lt; 7,50. Sedangkan klon-klon lainnya mempunyai koefisien keragaman &gt; 7,50. Berdasarkan hasil penelitian dan analisis data disimpulkan bahwa marka SSR dapat digunakan untuk menganalisis keragaman genetik plasma nutfah kakao. Tingkat polimorfisme yang dihasilkan marka SSR relatif tinggi. Tingkat heterosigositas plasma nutfah kakao koleksi Puslit Kopi dan Kakao Indonesiarelatif tinggi, dan keragaman genetiknyacukup tinggi.</p><p>Kata kunci : Theobroma cacao L, mikrosatelit, marka molekuler, keragaman genetik, heterosigositas</p>

Breeding for Multiple Disease Resistance in Cocoa (Theobroma cacao L.)

2012 ◽  
Vol 6 (4) ◽  
pp. 182-194 ◽  
Author(s):  
D. Nyadanu ◽  
R. Akromah ◽  
B. Adomako ◽  
H. Dzahini-Ob ◽  
A.Y. Akrofi ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Theobroma Cacao ◽  
Multiple Disease Resistance ◽  
Theobroma Cacao L

scholarly journals Morphological and genetic diversity of cacao (Theobroma cacao L.) in Uganda

2019 ◽  
Vol 25 (2) ◽  
pp. 361-375 ◽  
Author(s):  
David Gopaulchan ◽  
Lambert A. Motilal ◽  
Frances L. Bekele ◽  
Séverine Clause ◽  
James O. Ariko ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Theobroma Cacao ◽  
Theobroma Cacao L
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