scholarly journals In vitro screening of selected essential oils from medicinal plants acclimated to Benin for their effects on methane production from rumen microbial fermentation

2016 ◽  
Vol 15 (12) ◽  
pp. 442-450 ◽  
Author(s):  
B Kouazounde Jacques ◽  
Jin Long ◽  
A McAllister Tim ◽  
D Gbenou Joachim
Keyword(s):  
Medicinal Plants ◽  
Essential Oils ◽  
Methane Production ◽  
Microbial Fermentation ◽  
In Vitro Screening

In vitro Screening of Antibacterial and Antioxidant Activities of Essential Oils from Four Moroccan Medicinal Plants

2017 ◽  
Vol 18 (4) ◽  
pp. 1-10 ◽  
Author(s):  
Abdelhakim Bouyahya ◽  
Youssef Bakri ◽  
Abdeslam Et-Touys ◽  
Ahmed Talbaoui ◽  
Aya Khouchlaa ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Essential Oils ◽  
Antioxidant Activities ◽  
In Vitro Screening

Effects of essential oils from African basil on fermentation ofAndropogon gayanusgrass in the Artificial Rumen (RUSITEC)

2015 ◽  
Vol 95 (3) ◽  
pp. 425-431
Author(s):  
Jacques B. Kouazounde ◽  
Joachim D. Gbenou ◽  
Maolong He ◽  
Túlio Jardim ◽  
Long Jin ◽  
...  
Keyword(s):  
Essential Oils ◽  
Methane Production ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Microbial Fermentation ◽  
Total Production ◽  
Ruminal Fermentation ◽  
Microbial Protein ◽  
Andropogon Gayanus

Kouazounde, J. B., Gbenou, J. D., He, M., Jardim, T., Jin, L., Wang, Y., Beauchemin, K. A. and McAllister, T. A. 2015. Effects of essential oils from African basil on fermentation of Andropogon gayanus grass in the Artificial Rumen (RUSITEC). Can. J. Anim. Sci. 95: 425–431. Essential oils (EO) from African basil (Ocimum gratissimum) have shown the potential to modify rumen microbial fermentation and reduce ruminal methane production from grass forages in in vitro batch cultures. However, it is not known whether the effects of EO on rumen microbial fermentation attenuate over time. The objective of this study was to examine the effects of African basil EO at 0 (control), 100, 200 and 400 mg L−1incubation medium on microbial fermentation and methane production in the Rumen Simulation Technique (RUSITEC) using Andropogon gayanus grass as a substrate. African basil EO quadratically affected (P<0.05) methane production gas production and the pH of fermenter liquid. Total volatile fatty acid (VFA) production was linearly decreased (P<0.05) by African basil EO along with a shift in VFA profile towards less propionate and more acetate and butyrate. African basil EO quadratically altered (P<0.05) apparent dry matter, neutral detergent fiber digestibility,15N incorporation into total microbial protein and the total production of microbial protein. This study confirms that EO from African basil quadratically affected methane emissions arising from the ruminal fermentation of A. gayanus grass mainly by reducing overall digestibility of the forage.

Phytochemical composition and in vitro screening of the antimicrobial activity of essential oils on oral pathogenic bacteria

2017 ◽  
Vol 32 (5) ◽  
pp. 544-551 ◽  
Author(s):  
Roberta Tardugno ◽  
Federica Pellati ◽  
Ramona Iseppi ◽  
Moreno Bondi ◽  
Giacomo Bruzzesi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
In Vitro Screening ◽  
Phytochemical Composition

In vitro screening of South African medicinal plants in the pursuit of anti-viral agents against SARS-CoV-2

2021 ◽  
Author(s):  
Luke Invernizzi ◽  
Phanankosi Moyo ◽  
Ian Tietjen ◽  
Thomas Klimkait ◽  
Vinesh Maharaj
Keyword(s):  
Medicinal Plants ◽  
South African ◽  
In Vitro Screening

scholarly journals Modelling the impact of the macroalgae Asparagopsis taxiformis on rumen microbial fermentation

2020 ◽  
Author(s):  
Rafael Muñoz-Tamayo ◽  
Juana C. Chagas ◽  
Mohammad Ramin ◽  
Sophie J. Krizsan
Keyword(s):  
Methane Production ◽  
Volatile Fatty Acids ◽  
Mean Squared Error ◽  
Microbial Fermentation ◽  
Model Structure ◽  
Red Macroalgae ◽  
Asparagopsis Taxiformis ◽  
The Impact

AbstractBackgroundThe red macroalgae Asparagopsis taxiformis is a potent natural supplement for reducing methane production from cattle. A. taxiformis contains several anti-methanogenic compounds including bromoform that inhibits directly methanogenesis. The positive and adverse effects of A. taxiformis on the rumen microbiota are dose-dependent and operate in a dynamic fashion. It is therefore key to characterize the dynamic response of the rumen microbial fermentation for identifying optimal conditions on the use of A. taxiformis as a dietary supplement for methane mitigation. Accordingly, the objective of this work was to model the effect of A. taxiformis supplementation on the rumen microbial fermentation under in vitro conditions. We adapted a published mathematical model of rumen microbial fermentation to account for A. taxiformis supplementation. We modelled the impact of A. taxiformis on the fermentation and methane production by two mechanisms, namely (i) direct inhibition of the growth rate of methanogenesis by bromoform and (ii) hydrogen control on sugars utilization and on the flux distribution towards volatile fatty acids production. We calibrated our model using a multi-experiment estimation approach that integrated experimental data with six macroalgae supplementation levels from a published in vitro study assessing the dose-response impact of A. taxiformis on rumen fermentation.Resultsour model captured satisfactorily the effect of A. taxiformis on the dynamic profile of rumen microbial fermentation for the six supplementation levels of A. taxiformis with an average determination coefficient of 0.88 and an average coefficient of variation of the root mean squared error of 15.2% for acetate, butyrate, propionate, ammonia and methane.Conclusionsour results indicated the potential of our model as prediction tool for assessing the impact of additives such as seaweeds on the rumen microbial fermentation and methane production in vitro. Additional dynamic data on hydrogen and bromoform are required to validate our model structure and look for model structure improvements. We are working on model extensions to account for in vivo conditions. We expect this model development can be useful to help the design of sustainable nutritional strategies promoting healthy rumen function and low environmental footprint.

scholarly journals Effect of essential oils and aqueous extracts of plants on in vitro rumen fermentation and methane production

2022 ◽  
Vol 10 (1) ◽  
pp. 1-9
Author(s):  
Aarón A. Molho-Ortiz ◽  
Atmir Romero-Pérez ◽  
Efrén Ramírez-Bribiesca ◽  
Claudia C. Márquez-Mota ◽  
Francisco A. Castrejón-Pineda ◽  
...  
Keyword(s):  
Essential Oils ◽  
Methane Production ◽  
Rumen Fermentation ◽  
Aqueous Extracts ◽  
In Vitro Rumen Fermentation

In vitro screening of Indian medicinal plants for antiplasmodial activity

2001 ◽  
Vol 74 (2) ◽  
pp. 195-204 ◽  
Author(s):  
Henrik Toft Simonsen ◽  
Jesper Brændegaard Nordskjold ◽  
Ulla Wagner Smitt ◽  
Ulf Nyman ◽  
Pushpangadan Palpu ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Antiplasmodial Activity ◽  
In Vitro Screening ◽  
Indian Medicinal Plants

Essential oils from Lippia turbinata and Tagetes minuta persistently reduce in vitro ruminal methane production in a continuous-culture system

2019 ◽  
Vol 59 (4) ◽  
pp. 709 ◽  
Author(s):  
F. Garcia ◽  
P. E. Vercoe ◽  
M. J. Martínez ◽  
Z. Durmic ◽  
M. A. Brunetti ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Essential Oils ◽  
Acid Concentration ◽  
Methane Production ◽  
Volatile Fatty Acid ◽  
Crude Protein ◽  
Gas Production ◽  
Volatile Fatty Acid Concentration ◽  
Tagetes Minuta

The aim of the present study was to evaluate the impact of essential oils (EO) from Lippia turbinata (LT) and Tagetes minuta (TM) as well as the rotation of both EO on fermentation parameters in vitro. Daily addition of LT, TM, or a 3-day rotation between them (TM/LT), as well as a control (without EO), was evaluated using the rumen simulation technique (Rusitec). The experiment lasted 19 days, with a 7-day adaptation period, followed by 12 days of treatment (Days 0–12). The EO were dissolved in ethanol (70% vol/vol) to be added daily to fermenters (300 μL/L) from Day 0. Daily measurements included methane concentration, total gas production, apparent DM disappearance and pH, which started 2 days before the addition of treatments. On Days 0, 4, 8 and 12 apparent crude protein disappearance and neutral detergent fibre disappearance, ammonia and volatile fatty acid concentration and composition were determined. Methane production was significantly inhibited shortly after addition of both EO added individually, and persisted over time with no apparent adaptation to EO addition. The TM/LT treatment showed a similar effect on methane production, suggesting that rotating the EO did not bring further improvements in reduction or persistency compared with the inclusion of the EO individually. Gas production, total volatile fatty acid concentration and composition and apparent crude protein disappearance were not affected by EO addition. Compared with the control, a 5% reduction of apparent DM disappearance and a 15% reduction of neutral detergent fibre disappearance were observed with the addition of EO. Only TM and TM/LT reduced ammonia concentration. Given the significant and persistent antimethanogenic activity of both EO, and the potential of T. minuta to modify nitrogen metabolism, EO from these plant species are of interest for developing new feed additives with potential application in ruminant nutrition that are also likely to be acceptable to consumers.

In-vitro screening of medicinal plants for potential antidiabetic effects

1998 ◽  
Vol 50 (S9) ◽  
pp. 219-219 ◽  
Author(s):  
S. Srijayanta ◽  
P. M. Jones ◽  
S. Persaud ◽  
M. J. Lawrence ◽  
A. Raman
Keyword(s):  
Medicinal Plants ◽  
In Vitro Screening ◽  
Antidiabetic Effects
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