scholarly journals Evaluation of the antibacterial activity of Syzygium cordatum fruit-pulp and seed extracts against bacterial strains implicated in gastrointestinal tract infections

2015 ◽  
Vol 14 (16) ◽  
pp. 1387-1392 ◽  
Author(s):  
Tsolanku Sidney Maliehe ◽  
Jabulane Siyabonga Shandu ◽  
Albertus Kotze Basson
Keyword(s):  
Antibacterial Activity ◽  
Gastrointestinal Tract ◽  
Fruit Pulp ◽  
Bacterial Strains ◽  
Tract Infections ◽  
Seed Extracts

scholarly journals Phytochemical and Antibacterial Properties of Ethanolic Seed Extracts of Chrysophyllum albidum (African Star Apple)

2016 ◽  
Vol 1 (1-2) ◽  
pp. 05-09 ◽  
Author(s):  
Samuel L. Oputah ◽  
Kolawole O. Ajanaku ◽  
Raphael C. Mordi ◽  
Joseph A. O. Olugbuyiro ◽  
Shade J. Olorunshola ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Antibacterial Properties ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Zone Of Inhibition ◽  
Test Organisms ◽  
Seed Extracts ◽  
African Star Apple

Phytochemical and antibacterial properties of ethanolic extract of the seeds of African Star Apple (Chrysophyllum albidum) were investigated. The phytochemical result revealed the presence of saponins, carbohydrates, flavonoids, quinones, cardiac glycosides, fatty acids and terpenoids. The antibacterial activity was studied using agar well diffusion method at different concentrations against six pathogenic bacterial strains, three Gram-positive (Staphylococcus aureus, Micrococcus varians and Bacillus cereus) and three Gram-negative (Escherichia coli, Pseudomonas aeruginosa and Proteus vulgaris). Significant inhibitory activities were exhibited by the ethanolic seed extracts for all test organisms except Bacillus cereus. Zone of inhibition of the crude ethanolic extract was correlated with that of a standard antibiotic Gentamicin, for antibacterial activity. The results indicated a notable inhibition of the bacterial growth.

scholarly journals Iriflophenone-3-C-β-d Glucopyranoside from Dryopteris ramosa (Hope) C. Chr. with Promising Future as Natural Antibiotic for Gastrointestinal Tract Infections

Antibiotics ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1128
Author(s):  
Muhammad Ishaque ◽  
Yamin Bibi ◽  
Samha Al Ayoubi ◽  
Saadia Masood ◽  
Sobia Nisa ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Bioactive Compound ◽  
2D Nmr ◽  
Artemia Salina ◽  
Diffusion Method ◽  
Spectroscopic Techniques ◽  
Bacterial Strains ◽  
Aqueous Fraction ◽  
Tract Infections ◽  
Antibacterial Potential

Ethnopharmacological approaches provide clues for the search of bioactive compounds. Dryopteris ramosa (Hope) C. Chr. (plant family: Dryopteridaceae) is an ethnomedicinal plant of the Galliyat region of Pakistan. The aqueous fraction (AqF) of D. ramosa is being used by inhabitants of the Galliyat region of Pakistan to treat their gastrointestinal tract ailments, especially those caused by bacteria. The aims of the present study were as follows: (i) to justify the ethnomedicinal uses of the AqF of D. ramosa; (ii) to isolate a bioactive compound from the AqF of D. ramosa; and (iii) to evaluate the antibacterial and cytotoxic potential of the isolated compound. Column chromatography (CC) techniques were used for the isolation studies. Spectroscopic techniques (UV–Vis, MS, 1&2D NMR) were used for structural elucidation. The agar-well diffusion method was used to evaluate the antibacterial potential of “i3CβDGP” against five bacterial strains, and compare it with the known antibiotic “Cefixime”. The brine shrimp lethality test (BSLT) was used for cytotoxic studies. The AqF of D. ramosa afforded “iriflophenone-3-C-β-D glucopyranoside (i3CβDGP)” when subjected to LH20 Sephadex, followed by MPLC silica gel60, and purified by preparative TLC. The “i3CβDGP” showed a strong potential (MIC = 31.1 ± 7.2, 62.5 ± 7.2, and 62.5 ± 7.2 µg/mL) against Klebsiella pneumoniae, Staphylococcus aureus, and Escherichia coli, respectively. On the other hand, the least antibacterial potential was shown by “i3CβDGP” (MIC = 125 ± 7.2 µg/mL), against Bacillus subtilis, in comparison to Cefixime (MIC = 62.5 ± 7.2µg/mL). The cytotoxicity of “i3CβDGP” was significantly low (LD50 = 10.037 ± 2.8 µg/mL) against Artemia salina nauplii. This study not only justified the ethnomedicinal use of D. ramosa, but also highlighted the importance of ethnomedicinal knowledge. Further studies on AqF and other fractions of D. ramosa are in progress.

scholarly journals Antibacterial Activity of Some Nanoparticles Against Some Pathogenic Bacteria That Isolated From Urinary Tract Infections Patient

2019 ◽  
Vol 9 (04) ◽  
pp. 682-685
Author(s):  
Taha H. Alnasrawi ◽  
Zahraa A. Althabet ◽  
Ghufran S. Salih ◽  
Mohammad J. Al-Jassani
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Klebsiella Pneumonia ◽  
Bacterial Strains ◽  
High Concentration ◽  
Disk Diffusion Assay ◽  
Tract Infections ◽  
Diffusion Assay ◽  
Economical Alternative

Nanoparticles include multiple metal oxides such as yttrium, copper, nickel, zinc, iron, and silver that have antimicrobial activity. The present work evaluates the antibacterial activity of some nanoparticles (NPs)against various pathogenic bacterial strains (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia and Staphylococcus aureus). Antimicrobial activity of NPs was accomplished by the mean of disk diffusion assay using dilutions of (200, 100, 50, 25, and 12.5) and the MIC and MBC of each isolate is determined. NPs shows no antibacterial activity against tested bacteria. It is highly recommended using NPs as an economical alternative antibacterial agent, especially in treating ectopic infections but with high concentration since low concentration gives no result without taking the risk of developing resistant bacterial strains as with antibiotics.

scholarly journals Pharmacodynamic and cytotoxicity effects of Syzygium cordatum {S Ncik, 48 (UZ)} fruit-pulp extract in gastrointestinal tract infections

2017 ◽  
Vol 16 (6) ◽  
pp. 1349 ◽  
Author(s):  
Tsolanku S. Maliehe ◽  
Jabulane S. Shandu ◽  
Albertus K. Basson ◽  
Mthokosizi B. Simelane ◽  
G Lazarus ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Fruit Pulp ◽  
Cytotoxicity Effects ◽  
Tract Infections

scholarly journals Antibacterial activity of seneciolactone isolated from Senecio scandens against some common gastrointestinal tract disease causing bacterial pathogens

2016 ◽  
Vol 11 (1) ◽  
pp. 218
Author(s):  
Yi Zheng ◽  
Yu Wang ◽  
Yuan-Hong Shang
Keyword(s):  
Antibacterial Activity ◽  
Gastrointestinal Tract ◽  
Bacterial Pathogens ◽  
Cellular Membrane ◽  
Shigella Dysenteriae ◽  
Cellular Morphology ◽  
Bacterial Cells ◽  
Bacterial Strains ◽  
Zones Of Inhibition ◽  
Tract Disease

<p class="Abstract">The objective of the present investigation was to study the antibacterial effect of seneciolactone isolated from the methanolic extract of <em>Senecio scandens</em> against five bacterial pathogens which are known to cause several gastrointestinal tract diseases. Disc diffusion assay and agar well diffusion assays were used to examine the antibacterial efficacy of this compound by measuring zones of inhibition and MIC/MBC values. Scanning electron microscopy (SEM) was involved to study the effect of this compound on cellular morphology of <em>Shigella dysenteriae</em>. Results revealed that seneciolactone exhibited moderate to potent antibacterial activity against different bacterial strains. Zones of inhibition and MIC/MBC values indicated that seneciolactone was most potent against<em> S. dysenteriae</em> followed by <em>Pseudomonas aeruginosa</em> and <em>E. coli</em>. SEM results indicated that seneciolactone induced potent damage to the cell membrane of the tested bacteria. As compared to the untreated control which exhibited normal cellular morphology, the seneciolactone treated bacterial cells revealed severe damage to the cellular membrane particularly at the higher doses.</p><p> </p>

scholarly journals Antibacterial Activity of Locally Prepared Herbal Cough Extracts against Klebsiella pneumoniae and Streptococcus pneumoniae

2019 ◽  
pp. 1-9
Author(s):  
Atuheirwe Maxine ◽  
Jacob Stanley Iramiot
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Pneumoniae ◽  
Klebsiella Pneumoniae ◽  
Diffusion Method ◽  
Dilution Method ◽  
Herbal Extracts ◽  
Bacterial Strains ◽  
Zones Of Inhibition ◽  
Tract Infections

Aim: Cough due to Klebsiella pneumoniae and Streptococcus pneumoniae is currently managed by conventional antibiotics and herbal extracts in Uganda. However, much as these herbal extracts are extensively used, their antibacterial activity is not known. This study aimed at determining the antibacterial activity of the selected locally prepared herbal cough extracts against two bacterial strains i.e. Klebsiella pneumoniae (ATCC 700603), and Streptococcus pneumoniae (ATCC 49619). Methods: The herbal cough extracts were screened for antibacterial activity using Agar-well diffusion method for determining zone of inhibition, macro broth dilution method for Minimum Inhibitory Concentration (MIC) determination and Streak plate method for Minimum Bactericidal Concentration (MBC). Results: In vitro evaluation of antibacterial activity of the 5 brands of herbal cough extracts against K. pneumoniae and S. pneumoniae   revealed that all extracts possessed significant antimicrobial effects against all microorganisms tested (p < 0.05). However, MM04 (35.6±0.0) mm and MM03 (33.6±1.5) mm had maximum zones of inhibition as compared to other herbal extracts against K. pneumoniae and S. pneumoniae respectively. Average MIC results for extracts against K. pneumoniae   indicated that MM01 had the highest MIC (2.5000 mg/ml) while MM03 had the least MIC (0.0625 mg/ml). Average MIC results for extracts against S. pneumoniae showed MM01 had the highest MIC (2.0000 mg/ml) while MM03 3 had the least MIC (0.0438 mg/ml). Average MBC results for extracts against K. pneumoniae indicated that MM01 had the highest MBC (4.000 mg/ml) while MM03 had the least MBC (0.030 mg/ml). Average MBC results for extracts against S. pneumoniae showed MM01 had the highest MBC (4.000 mg/ml) while MM03 had the least MBC (0.033 mg/ml). Conclusion: The results obtained in present study were revealed that locally prepared herbal extracts had significant antibacterial activity. Hence they can be used as promising alternatives of antibiotics used against Respiratory Tract Infections due to K. pneumoniae and S. pneumoniae.

scholarly journals Seeds extract of three Artocarpus species: Their in-vitro antibacterial activities against multidrug-resistant (MDR) Escherichia coli isolates from urinary tract infections (UTIs)

2021 ◽  
Vol 22 (10) ◽  
Author(s):  
Muhammad Evy Prastiyanto
Keyword(s):  
Antibacterial Activity ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Antibacterial Agents ◽  
Antibacterial Activities ◽  
Multidrug Resistant ◽  
E Coli ◽  
Tract Infections ◽  
Seed Extracts

Abstract. Prastiyanto ME. 2021. Seeds extract of three Artocarpus species: Their in-vitro antibacterial activities against multidrug-resistant (MDR) Escherichia coli isolates from urinary tract infections (UTIs). Biodiversitas 22: 4362-4368. Multidrug-resistant (MDR)-E. coli is a major cause and has become a very serious problem in urinary tract infections (UTIs). As a result, it requires an antibacterial agent derived from biological materials. It has been reported that the seeds of three species of Artocarpus (A. heterophyllous, A. champeden, and A. camansi) have antibacterial properties against Methicillin-Resistant Staphylococcus aureus (MRSA). However, there are three other Artocarpus species in Indonesia, i.e., keledang (A. lanceipolius), tarra (A. elasticus), and terap (A. Odoratissimus) whose antibacterial property has not been investigated. To minimize the research gap, this study aims to determine the antibacterial activity of seed extracts of A. lanceipolius, A. elasticus, and A. odoratissimus against MDR-E. coli isolates of UTIs. Antibacterial activity was evaluated using the agar well diffusion assay. The microdilution method was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values. The results revealed that the seed extracts of A. lanceipolius, A. elasticus, and A. odoratissimus have the potential as antibacterial agents against MDR-E. coli isolate of UTIs. A. elasticus seed extract shows the widest zone of inhibition in the range of 7.0-13.3 mm and the smallest MIC and MBC values ??of 6.25-12.5 mg/mL and 12.5-25 mg/mL, respectively. In conclusion, A. lanceipolius, A. elasticus, and A. odoratissimus seed extracts have the potential to be developed as antibacterial agents against UTI-causing MDR-E. coli. Further in vivo research and determining the mode of action of antibacterial activity are needed.

scholarly journals Adhesive and hydrophobic properties of the selected LAB isolated from gastrointestinal tract of farming animals.

2016 ◽  
Vol 63 (2) ◽  
Author(s):  
Katarzyna Grajek ◽  
Anna Sip ◽  
Joanna Foksowicz-Flaczyk ◽  
Anna Dobrowolska ◽  
Agnieszka Wita
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Lactic Acid ◽  
Gastrointestinal Tract ◽  
Lactic Acid Bacteria ◽  
Clostridium Perfringens ◽  
Pathogenic Bacteria ◽  
Bacterial Strains ◽  
Hydrophobic Properties ◽  
Adhesion Ability

The aim of this study was to determine the degree of adhesion and hydrophobicity of some strains of lactic acid bacteria (LAB) with proven antagonistic properties against pathogenic bacteria. Studies were performed using-LAB strains isolated from the gastrointestinal tract of calves and piglets. These strains exhibited an antibacterial activity against pathogenic strains of Clostridium perfringens and Escherichia coli. Cell adhesiveness was examined in relation to the porcine and bovine mucin. Our analyses had shown that the tested microorganisms demonstrated a degree of adhesion in the range of 32.00-40.00% for strains isolated from calves, and 34.00-40.00% for strains isolated from pigs. The hydrophobicity of tested bacteria was in the range of 31.00%-44.00% for strains isolated from pigs, and 26.00%-42.00% for strains obtained from calves. The best mucin adhesion ability was found for bacterial strains belonging to the Enterococcus genus isolated from calves. Taking into account porcine isolates, the best adhesion was observed for the Leuconostoc bacterial strains. Among tested strains, the highest hydrophobicity, measured in relation to hexadecane, was recorded for the bacterial strains belonging to the Leuconostoc sp. isolated from piglets and a of Lactobacillus sp. bacterial strain isolated from calves.

scholarly journals The antibacterial activity of seagrass Enhalus acoroides against Staphylococcus aureus

2021 ◽  
Vol 890 (1) ◽  
pp. 012013
Author(s):  
R M Nur ◽  
Nurafni ◽  
K Koroy ◽  
D Alwi ◽  
I Wahab ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Gastrointestinal Tract ◽  
Bioactive Compounds ◽  
Methanol Extract ◽  
The Body ◽  
Hexane Extract ◽  
Skin Tissue ◽  
Enhalus Acoroides ◽  
Tract Infections

Abstract Enhalus acoroides is a type of seagrass found in the waters surrounding the Morotai Islands but has not been economically exploited. Enhalus acoroides can be used to treat ulcers, acne medications, and cosmetics, among other things, due to their antibacterial activity against a variety of bacteria, one of which is Staphylococcus aureus. Staphylococcus aureus can cause respiratory and gastrointestinal tract infections. Staphylococcus aureus can infect any tissue in the body, causing damage to skin tissue. This study aimed to determine the antibacterial activity of E. acoroides against S. aureus and characterize the group of bioactive compounds found in the E. acoroides extract. This study took place between September and October of 2018. Enhalus acoroides samples were collected from the waters surrounding Morotai island. Extraction of E. acoroides in maceration using methanol and n-hexane solvents and qualitative identification of bioactive compounds including alkaloid compounds, flavonoids, steroids, and saponins. The results indicated that the yield of E. acoroidesn-hexane extract was 0.35 percent and Methanol extract was 5.91 percent. Methanol extract E. acoroides (5.9 mm in diameter)1000 ppm is more antibacterial than n-hexane extract E. acoroides (5.6 mm in diameter). The Methanol extract of E. acoroides contains alkaloids, flavonoids, saponins, and steroids, whereas the n-hexane extract of E. acoroides contains steroids and flavonoids.

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