Interaction between faba bean cultivars and the Rhizobium leguminosarum strains: Symbiotic N2 fixation and protein profiles under salt stress

2014 ◽  
Vol 13 (41) ◽  
pp. 4081-4092
Author(s):  
Shoukry Adel ◽  
Abou El-Lail Mohamed ◽  
El-Shabrawi Hattem ◽  
El-Nasser Khattab Abd ◽  
Abbas Mohamed
Keyword(s):  
Salt Stress ◽  
Faba Bean ◽  
N2 Fixation ◽  
Rhizobium Leguminosarum ◽  
Symbiotic N2 Fixation ◽  
Protein Profiles

Intercropping alleviates the inhibitory effect of N fertilization on nodulation and symbiotic N2 fixation of faba bean

2009 ◽  
Vol 323 (1-2) ◽  
pp. 295-308 ◽  
Author(s):  
Yu-Ying Li ◽  
Chang-Bin Yu ◽  
Xu Cheng ◽  
Chun-Jie Li ◽  
Jian-Hao Sun ◽  
...  
Keyword(s):  
Faba Bean ◽  
N2 Fixation ◽  
Inhibitory Effect ◽  
N Fertilization ◽  
Symbiotic N2 Fixation

Effects of Rhizobium Strain on the Growth, Nodulation, N2 Fixation and Ions Accumulation in Vicia Faba Plant Under Salt Stress

2019 ◽  
Author(s):  
Boulbaba L’taief ◽  
Neila Abdi ◽  
Sihem Smari ◽  
Amel Ayari-Akkari ◽  
Mouna Jeridi ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Vicia Faba ◽  
N2 Fixation ◽  
Rhizobium Leguminosarum ◽  
Solution Culture ◽  
Phosphorus Concentration ◽  
Rhizobium Strain ◽  
Faba Beans ◽  
Stable Growth

Vicia faba L.-rhizobia symbiosis is utilized in different biological ways to improve the productivity of faba beans. This research aims to analyze the effects of the Rhizobium strain on nodulation, N2 fixation, growth, and ion accumulation under salt stress in Viciafaba. The commercial cultivar of faba beans (Viciafaba L. var. minor) was inoculated with the Rhizobium leguminosarumbiovar, by considering viciae strains S10 and S16, after 15 days of growth. This inoculation was carried out in the solution culture consisting of two salt concentrations; 0 mmole l-1NaCl and 50 mmole l-1NaCl. The results revealed that under saline and non-saline conditions, S10 and S16 strains of Rhizobium leguminosarum resulted in the formation of ineffective and effective symbiosis with faba beans. However, the presence of salt stress resulted in increasing the biomass of nodule and nitrogen content. The concentrations of sodium and chloride, in shoot and root, were increased in the presence of salinity. However, potassium concentration was only increased in the shoot. With and without salinity, phosphorus concentration in the shoots was not modified. The results revealed that the salt tolerance of faba beans, inoculated with two strains of Rhizobium were found to possess association with their stable growth. Moreover, the salt tolerance of faba beans inoculated with two salts tolerant rhizobia was also associated with an increment in the capacity of faba beans to increase nodulation and the concentration of shoot N2, Na and Cl-content. In addition, salt tolerance of this variety, inoculated with Rhizobium strains was associated with a decrement in the concentration of K+ in shoot under the salt constraints.

scholarly journals Enhanced Abiotic Stress Tolerance of Vicia faba L. Plants Heterologously Expressing the PR10a Gene from Potato

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 173
Author(s):  
Abeer F. Desouky ◽  
Ahmed H. Ahmed ◽  
Hartmut Stützel ◽  
Hans-Jörg Jacobsen ◽  
Yi-Chen Pao ◽  
...  
Keyword(s):  
Salt Stress ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Vicia Faba ◽  
Faba Bean ◽  
Abiotic Stress Tolerance ◽  
Wild Type ◽  
Stress Injury ◽  
Bean Plants ◽  
Vicia Faba L

Pathogenesis-related (PR) proteins are known to play relevant roles in plant defense against biotic and abiotic stresses. In the present study, we characterize the response of transgenic faba bean (Vicia faba L.) plants encoding a PR10a gene from potato (Solanum tuberosum L.) to salinity and drought. The transgene was under the mannopine synthetase (pMAS) promoter. PR10a-overexpressing faba bean plants showed better growth than the wild-type plants after 14 days of drought stress and 30 days of salt stress under hydroponic growth conditions. After removing the stress, the PR10a-plants returned to a normal state, while the wild-type plants could not be restored. Most importantly, there was no phenotypic difference between transgenic and non-transgenic faba bean plants under well-watered conditions. Evaluation of physiological parameters during salt stress showed lower Na+-content in the leaves of the transgenic plants, which would reduce the toxic effect. In addition, PR10a-plants were able to maintain vegetative growth and experienced fewer photosystem changes under both stresses and a lower level of osmotic stress injury under salt stress compared to wild-type plants. Taken together, our findings suggest that the PR10a gene from potato plays an important role in abiotic stress tolerance, probably by activation of stress-related physiological processes.

scholarly journals Exogenous Auxin-Mediated Salt Stress Alleviation in Faba Bean (Vicia faba L.)

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 547
Author(s):  
Arafat Abdel Hamed Abdel Latef ◽  
Md. Tahjib-Ul-Arif ◽  
Mohammad Saidur Rhaman
Keyword(s):  
Salt Stress ◽  
Vicia Faba ◽  
Faba Bean ◽  
Stress Responses ◽  
Foliar Application ◽  
Soluble Sugar ◽  
Principal Component ◽  
Vicia Faba L ◽  
Catalase Peroxidase ◽  
Amino Acid Contents

Auxin not only controls the development processes, but also regulates the stress responses of plants. In this investigation, we explored the potential roles of exogenously applied indole-3-acetic acid (IAA) in conferring salt tolerance in the faba bean (Vicia faba L.). Our results showed that foliar application of IAA (200 ppm) to salt-exposed (60 mM and 150 mM NaCl) plants promoted growth, which was evidenced by enhanced root–stem traits. IAA application ensured better osmotic protection in salt-stressed plants which was supported by reduced proline and enhanced soluble sugar, soluble protein, and total free amino acid contents in the roots, stem, and seeds. IAA application also increased the number of nodules in salt-stressed plants, which may facilitate better nitrogen assimilation. Moreover, IAA mediated improvements in mineral homeostasis (K+, Ca2+, and Mg2+) and the translocation of Na+, while it also inhibited excessive accumulation of Na+ in the roots. Salt-induced oxidative damage resulted in increased accumulation of malondialdehyde, whereas IAA spraying relegated malondialdehyde by improving antioxidant enzymes, including superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase. Together, these results together with a principal component analysis uncovered that foliar spraying of IAA alleviated the antagonistic effects of salt stress via enhancing osmolyte accumulation, ionic homeostasis, and antioxidant activity. Finally, exogenous IAA enhanced the yield of broad beans under high salinity conditions.

Estimating Nitrogenase Activity of Faba Bean (Vicia faba) by Acetylene Reduction (Ar) Assay

1990 ◽  
Vol 17 (5) ◽  
pp. 489 ◽  
Author(s):  
Herdina ◽  
JH Silsbury
Keyword(s):  
Diurnal Variation ◽  
Vicia Faba ◽  
Faba Bean ◽  
Closed System ◽  
N2 Fixation ◽  
Acetylene Reduction ◽  
Nitrogenase Activity ◽  
Electron Flow ◽  
Specific Rate ◽  
Rooting Medium

Methods of conducting acetylene reduction (AR) assay were appraised for estimating the nitrogenase activity of nodules of faba bean (Vicia faba L.). Factors considered were: (i) disturbance of plants when removing the rooting medium; (ii) assay temperature; (iii) the use of whole plants rather than detached, nodulated roots; (iv) diurnal variation in nodule activity; and (v) a decline in C2H4 production after exposure to C2H2. Plants growing in jars of 'oil dry' (calcined clay) had the same AR activity when assayed in situ in a closed system as when assayed after removal of the rooting medium. Assay temperatures of 12.5, 17.5 and 22.5°C influenced the specific rate of AR with the optimum at 17.5°C. Removal of the shoot resulted in a rapid decrease in AR activity in both vegetative and reproductive plants but the effect was much larger in the latter. AR and respiration by nodulated roots were closely linked and both varied markedly over a diurnal 12 h/12 h cycle. Since no fluctuation was found after nodules were detached, diurnal variation in the respiration of nodulated roots is attributed to change in nodule activity. Half of the dark respiration of nodulated roots was associated with respiration of the nodules and thus largely with N2 fixation. Since the AR assay provides no information on how electron flow in vivo is partitioned between reduction of N2 and reduction of protons, diurnal variation in hydrogen evolution (HE) in air and Ar/O2 in an open system was used to estimate this partitioning. Diurnal variation in apparent N2 fixation estimated in this manner was examined at a 'low' PPFD (300 μmol m-2 s-1) and at 'high' (1300 μmol m-2 s-1) to explore whether variation could be attributed to change in carbohydrate supply. Although HE in air and in Ar/O2 were both closely linked with the respiration of the nodulated root, apparent N2 fixation showed only a slight diurnal variation at 'low' light and almost none at 'high'. Vegetative plants showed no C2H2-induced decline in activity with exposure to C2H2 but reproductive plants did. This difference appears to be an age effect rather than attributable to flowering per se, since a decline occurred even when plants were kept vegetative by disbudding. A closed system for AR assay appears satisfactory for vegetative faba bean but such an assay over a 40-min period during the reproductive stage would underestimate nitrogenase activity by about 20%.

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