scholarly journals Identification of putative candidate gene markers for grain zinc content using recombinant inbred lines (RIL) population of IRRI38 X Jeerigesanna

2014 ◽  
Vol 13 (5) ◽  
pp. 657-663 ◽  
Author(s):  
Kumar Gande Naveen ◽  
J Kundur Pavan ◽  
Soman Rakhi ◽  
Ambati Rajeswari ◽  
R Ashwathanarayana ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Zinc Content ◽  
Inbred Lines ◽  
Gene Markers ◽  
Grain Zinc ◽  
Ril Population ◽  
Putative Candidate Gene ◽  
Candidate Gene Markers

scholarly journals Solanum habrochaites Accession LA1777 Recombinant Inbred Lines Are Not Resistant to Tomato Yellow Leaf Curl Virus or Tomato Mottle Virus

HortScience ◽  
2007 ◽  
Vol 42 (5) ◽  
pp. 1149-1152 ◽  
Author(s):  
Aliya Momotaz ◽  
John W. Scott ◽  
David J. Schuster
Keyword(s):  
Disease Severity ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Tomato Yellow Leaf ◽  
Mottle Virus ◽  
Yellow Leaf ◽  
Ril Population ◽  
Leaf Curl Virus ◽  
Leaf Curl

Cultivated tomato (Solanum lycopersicum L.) accessions have been susceptible to the whitefly-transmitted begomoviruses Tomato yellow leaf curl virus (TYLCV) and Tomato mottle virus (ToMoV) that can cause serious crop damage. S. habrochaites accession LA1777 has been reported to be resistant to TYLCV. To locate putative virus resistance genes, 89 recombinant inbred lines (RILs) previously developed from LA1777 in a tomato background, LA1777 and the susceptible RIL parent E6203, were screened against the begomoviruses TYLCV and ToMoV. An initial study showed 18 RILs had less disease severity to TYLCV or ToMoV. Eight RILs had S. habrochaites alleles at TG27 (restriction fragment length polymorphism marker) on chromosome 1, three RILs had S. habrochaites alleles at TG202 on chromosome 7, and one RIL had S. habrochaites alleles at both marker loci. The RILs with these regions were intercrossed in 10 different cross combinations and F2 seeds were then obtained. The F2 progenies were inoculated separately with both viruses and then evaluated in the field. The F2 plants with less disease severity were selected, but most did not have the markers from the hypothetical resistance regions. The F3 progenies were then inoculated and rated for disease severity to both viruses. None of the F3s demonstrated any increased level of resistance, even if derived from F2s homozygous for the target regions from both chromosomes. All plants from every cross combination were susceptible for both TYLCV and ToMoV, suggesting that there is no begomovirus resistance in the LA1777 RIL population. Some limitations of capturing all genes in an RIL population derived from an outcrossing accession are discussed.

scholarly journals Morphological and molecular characterization of recombinant inbred lines for dormancy related traits in rice (Oryza sativa L.)

2021 ◽  
Vol 17 (AAEBSSD) ◽  
pp. 41-50
Author(s):  
D. B. K. V. Mani ◽  
B. Krishna Veni ◽  
V. Roja ◽  
S. N. Umar
Keyword(s):  
Molecular Characterization ◽  
Banding Pattern ◽  
Recombinant Inbred ◽  
Genetic Relatedness ◽  
Oryza Sativa L ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Polymorphic Markers ◽  
Ril Population ◽  
Morphological And Molecular Characterization

The study was carried out to assess the genetic relatedness of 119 recombinant inbred lines along with their parents BPT 2231and MTU 1001 based on morphological and molecular characterization. Data recorded for 10 morphological characters was used for grouping genotypes into different clusters by using Tocher’s and UPGMA dendrogram methods. Under D2analysis, the genotypes were grouped into 6 clusters and out of 10 characters studied, free amino acids contributed maximum divergence followed by days to 50% flowering and test weight. The genotypes grouped in cluster III recorded strong dormancy both at 5 days and 10 days after harvesting whereas the genotypes of cluster IV recorded maximum grain yield/plant. Four polymorphic markers were used for genotyping of the RIL population and the lines viz.,SD 3, SD 12, SD 15, SD 68, SD 72, SD 87 and SD 113 which were categorized as strongly dormant upto 10 days after harvesting also exhibited similar banding pattern with the dormant parent MTU 1001 when amplified with more than two markers. Among the 119 RIL population studied, SD 12 manifested banding pattern as that of the dormant parent MTU 1001 with all the four polymorphic markers viz., RM346, RM22565, RM7051 and RM10793 under study and also exhibited <10% germination at 10 days after harvesting. The number of alleles detected for each of the 4 polymorphic markers ranged from 2 to 4 per loci with mean value of 2.75 alleles per locus. The dendrogram analysis divided the entire population into two distinct clusters at the distance of 2.25. The RILs viz., SD11, SD12, SD15, SD27, SD30, SD63, SD65, SD68, SD74, SD75, SD80 along with the dormant parent MTU 1001(SD 121) which recorded less than 50% germination and categorized as strongly dormant under phenotyping were grouped in cluster IB2 in molecular analysis thus revealing the similarity between the two methods.

Diverse sources of resistance to Spodoptera litura (F.) in groundnut

2019 ◽  
Vol 79 (01S) ◽  
Author(s):  
M. A. Saleem ◽  
G. K. Naidu ◽  
H. L. Nadaf ◽  
P. S. Tippannavar
Keyword(s):  
Recombinant Inbred ◽  
Spodoptera Litura ◽  
Hot Spot ◽  
Recombinant Inbred Lines ◽  
Insect Pest ◽  
Inbred Lines ◽  
Leaf Damage ◽  
Botanical Variety ◽  
Resistant Genotypes ◽  
Botanical Varieties

Spodoptera litura an important insect pest of groundnut causes yield loss up to 71% in India. Though many effective chemicals are available to control Spodoptera, host plant resistance is the most desirable, economic and eco-friendly strategy. In the present study, groundnut mini core (184), recombinant inbred lines (318) and elite genotypes (44) were studied for their reaction to Spodoptera litura under hot spot location at Dharwad. Heritable component of variation existed for resistance to Spodoptera in groundnut mini core, recombinant inbred lines and elite genotypes indicating scope for selection of Spodoptera resistant genotypes. Only 29 (15%) genotypes belonging to hypogaea, fastigiata and hirsuta botanical varieties under mini core set, 15 transgressive segregants belonging to fastigiata botanical variety among 318 recombinant inbred lines and three genotypes belonging to hypogaea and fastigiata botanical varieties under elite genotypes showed resistance to Spodoptera litura with less than 10% leaf damage. Negative correlation existed between resistance to Spodoptera and days to 50 per cent flowering indicating late maturing nature of resistant genotypes. Eight resistant genotypes (ICG 862, ICG 928, ICG 76, ICG 2777, ICG 5016, ICG 12276, ICG 4412 and ICG 9905) under hypogaea botanical variety also had significantly higher pod yield. These diverse genotypes could serve as potential donors for incorporation of Spodoptera resistance in groundnut.

scholarly journals QTL analysis of seed dormancy in Arabidopsis using recombinant inbred lines and MQM mapping

Heredity ◽  
1997 ◽  
Vol 79 (2) ◽  
pp. 190-200 ◽  
Author(s):  
Wybe van der Schaar ◽  
Carlos Alonso-Blanco ◽  
Karen M Léon-Kloosterziel ◽  
Ritsert C Jansen ◽  
Johan W van Ooijen ◽  
...  
Keyword(s):  
Seed Dormancy ◽  
Qtl Analysis ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Inbred Lines
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