scholarly journals Influence of culture medium and age of zygotic embryos on in vitro germination of Elaeis guineensis Jacq.

2014 ◽  
Vol 13 (14) ◽  
pp. 1515-1523 ◽  
Author(s):  
Souza Pdua Marlcia ◽  
Vilela Paiva Luciano ◽  
Ferreira Pires Marins ◽  
Gustavo Texeira Da Silva Luiz ◽  
Mauro De Castro Evaristo ◽  
...  
Keyword(s):  
Culture Medium ◽  
Elaeis Guineensis ◽  
Zygotic Embryos ◽  
In Vitro Germination ◽  
Elaeis Guineensis Jacq

Mature zygotic embryo rescue improves in vitro germination and seedling production in high value oil palm (Elaeis guineensis Jacq.) cultivars

2016 ◽  
Vol 94 ◽  
pp. 445-453 ◽  
Author(s):  
Kingsley Tabi Mbi ◽  
Libert Brice Tonfack ◽  
Godswill Ntsomboh Ntsefong ◽  
Bilal Ahmad Mir ◽  
Georges Frank Ngando Ebongue ◽  
...  
Keyword(s):  
Oil Palm ◽  
Elaeis Guineensis ◽  
Zygotic Embryo ◽  
Embryo Rescue ◽  
In Vitro Germination ◽  
Seedling Production ◽  
Elaeis Guineensis Jacq ◽  
Mature Zygotic Embryo

scholarly journals In vitro germination of zygotic embryos of hybrid BRS Manicoré (E. guineensis X E. oleifera)

2016 ◽  
Vol 88 (3 suppl) ◽  
pp. 1841-1850
Author(s):  
KEILA A.P. BONETTI ◽  
MARGUERITE QUOIRIN ◽  
REGINA C. QUISEN ◽  
SUELEN C.S. LIMA
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Germination Rate ◽  
Phosphate Concentration ◽  
Zygotic Embryos ◽  
In Vitro Germination ◽  
N6 Medium ◽  
Whole Plants ◽  
Tetrazolium Test

ABSTRACT The interspecific oil palm hybrid BRS Manicoré (E. guineensis x E. oleifera) has superior agronomic characteristics. However, the germination rate is low (30%) and the process is slow when the seeds are sown in a conventional form. The purpose of this study was to optimize the in vitro germination of zygotic embryos of this hybrid comparing seed lots. The viability of zygotic embryos was evaluated by the tetrazolium test (0.075%) for 4 h. The embryos were cultured on MS and Y3 culture media, with and without the addition of NaH2PO4, as well as on MS, MS1/2 and N6 medium. In MS medium containing NaH2PO4, the germination rate was increased from 40 to 70% in comparison with the medium without sodium phosphate. The comparison between the culture media MS, MS 1/2, N6 and Y3 showed that 75% of zygotic embryos cultured in the Y3 medium formed whole plants (with roots and shoots defined), a higher percentage than embryos cultured on MS, MS 1/2 and N6 media (46, 35 and 17% respectively). In the same Y3 culture medium, the embryos were larger (36% ≥ 2 cm and 30% ≥ 5 cm) than in the other media. Results obtained by the tetrazolium test were similar to those of germination, showing the effect of the genotype of each seed lot. For the germination and development of plantlets it is essential to add NaH2PO4 to a culture medium containing no phosphate or with a low phosphate concentration.

scholarly journals Data on germination, growth and morphological changes of oil palm (Elaeis guineensis Jacq.) zygotic embryos during in vitro culturing

Data in Brief ◽  
2020 ◽  
Vol 28 ◽  
pp. 104975
Author(s):  
D.S. Sparjanbabu ◽  
H.S. Prasanna ◽  
D. Ramajayam ◽  
P. Naveenkumar ◽  
M.S.R. Krishna ◽  
...  
Keyword(s):  
Oil Palm ◽  
Elaeis Guineensis ◽  
Morphological Changes ◽  
Zygotic Embryos ◽  
Elaeis Guineensis Jacq

scholarly journals Effect of culture media, plant growth regulators and genotypes on growth and developmental stages of oil palm (Elaeis guineensis Jacq.) zygotic embryos

2019 ◽  
Author(s):  
D.S. Sparjanbabu ◽  
P. Naveen Kumar ◽  
M.S.R. Krishna ◽  
D. Ramajayam ◽  
B. Kalyana Babu ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Seed Production ◽  
Growth Regulators ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Culture Media ◽  
Zygotic Embryos ◽  
Elaeis Guineensis Jacq

Oil palm (Elaeis guineensis Jacq.) has become the largest traded vegetable oil in the world due to its innumerable uses and its huge economic importance. Which increases the requirement of quality seedling but the conventional seed production limits the commercial seed production with its long germination period and low germination rate. In this context in vitro germination of zygotic embryo could be a promising alternative. Hence in this study the effect of culturing media, plant growth regulators and genotypes were assessed on in vitro regeneration and plantlet development of oil palm zygotic embryos. Zygotic embryos of four elite genotypes were cultured on MS, N6 and Y3 media, with or without plant growth regulators (NAA, BA and GA3-0.2mg/lt). There was no effect of culture media, plant growth regulators and genotypes on germination of zygotic embryos. Whereas on the growth and development of in vitro plantlets, there was a significant effect of culture media and genotypes. Where culture media N6 performs well and Y3 has shown more abnormality. In case of genotypes, genotypes G1 and G2 shown better performance when compared to genotypes G3 and G4. The effect of plant growth regulators on germination and plant growth was non significant.

scholarly journals In vitro culture of zygotic embryos and seeds of Caesalpinia ferrea Martius

Hoehnea ◽  
2018 ◽  
Vol 45 (4) ◽  
pp. 663-668
Author(s):  
Daniel da Silva ◽  
Angela Maria Imakawa ◽  
Suely de Souza Costa ◽  
Paulo de Tarso Barbosa Sampaio
Keyword(s):  
In Vitro Culture ◽  
Culture Medium ◽  
Germination Rate ◽  
Zygotic Embryos ◽  
Multiplication Rate ◽  
Ms Medium ◽  
In Vitro Germination ◽  
Growth Room ◽  
Morphogenetic Responses

ABSTRACT The aim of this study was to evaluate the in vitro germination of zygotic embryos and seeds of Caesalpinia ferrea Martius and the morphogenetic responses of the explants to different concentrations of growth regulators. Seeds and zygotic embryos were inoculated in MS culture medium and kept in a growth room at a temperature of 25 ± 2 ºC for 16 hours of photoperiod for 30 days. The seeds had a higher in vitro germination rate than the explants from zygotic embryos. However, zygotic embryos in MS medium supplemented with 0.9 mg L-1 BAP had the highest percentage of regeneration (50%), number of shoots (3.25), buds (2.85) and leaves (3.15), multiplication rate (27.75), and length of shoots (1.96 cm). The in vitro culture of zygotic embryos and seeds made possible the multiplication of a higher number of healthy seedlings. Thus, it can be used as an alternative technique for the propagation of this species.

scholarly journals Accelerated in vitro propagation of elite oil palm genotypes (Elaeis guineensis Jacq.) by substituting cytokinin with putrescine

2016 ◽  
Vol 15 (50) ◽  
pp. 2767-2775 ◽  
Author(s):  
Roseli Correa Thais ◽  
Yoshimitsu Motoike Sérgio ◽  
Paula de Souza Andrade Ana ◽  
Morra Coser Sara ◽  
Queiroz Vanessa ◽  
...  
Keyword(s):  
Oil Palm ◽  
In Vitro Propagation ◽  
Elaeis Guineensis ◽  
Elaeis Guineensis Jacq

scholarly journals ACTIVATED CHARCOAL APPLICATION FOR THE MICROPROPAGATION OF Cattleya crispata (Thunb.) Van den Berg

Nativa ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 352-358
Author(s):  
Denys Matheus Santana Costa Souza ◽  
Sérgio Bruno Fernandes ◽  
Letícia Vaz Molinari ◽  
Maria Lopes Martins Avelar ◽  
Gilvano Ebling Brondani
Keyword(s):  
Endemic Species ◽  
Activated Charcoal ◽  
Culture Medium ◽  
Genetic Conservation ◽  
Campo Rupestre ◽  
In Vitro Germination ◽  
Germination Speed

 Micropropagation is an alternative for the genetic conservation and propagation of endemic species from “Campo Rupestre Ferruginoso”, such as the orchid Cattleya crispata. The aim of the present study is to assess the influence of activated charcoal on the in vitro germination, multiplication and elongation phases of C. crispata. Seeds extracted from mature capsules were used for inoculation in the culture medium that was adopted to assess the effect of supplementation, or not, with activated charcoal. Data about germination speed, seedling number, length, vigor, oxidation and contamination (bacterial and/or fungal) were assessed through these phases. Based on the results obtained, the use of activated charcoal was efficient in the in vitro germination and multiplication phases of C. crispata, providing greater speed and percentage of germination, less contamination and oxidation of the tissues, greater number, length and vigor of shoots, being effective for the genetic conservation and production of seedlings of the species. Culture medium without the supplementation of activated charcoal provided the best results for the in vitro elongation, with greater length, vigor and less oxidation of shoots.

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