scholarly journals Sensibility of the entomopathogenic fungus Metarhizium anisopliae (Metschnikoff) Sorokin to Pelargonium sidoides extract (EPs 7630) assessed by conidia germination speed parameter

2014 ◽  
Vol 13 (7) ◽  
pp. 821-826
Author(s):  
Tognolli de Almeida Tiago ◽  
Casarotti Orlandelli Ravely ◽  
Specian Vnia ◽  
Cesar Polonio Julio ◽  
Andressa Lino Loureno Daniela ◽  
...  
Keyword(s):  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Conidia Germination ◽  
Pelargonium Sidoides ◽  
Germination Speed ◽  
Speed Parameter

scholarly journals Toxicity of β-(1→3,1→6)-ᴅ-glucans produced by Diaporthe sp. endophytes on Metarhizium anisopliae (Metschnikoff) Sorokin assessed by conidia germination speed parameter

2020 ◽  
Vol 36 (5) ◽  
Author(s):  
Ravely Casarotti Orlandelli ◽  
Tiago Tognolli de Almeida ◽  
Daniela Andressa Lino Lourenco ◽  
Ana Flora Dalberto Vasconcelos ◽  
Maria de Lourdes Corradi Da Silva ◽  
...  
Keyword(s):  
Bayesian Analysis ◽  
Metarhizium Anisopliae ◽  
Model Systems ◽  
Human Breast ◽  
Important Data ◽  
Antiproliferative Agents ◽  
Conidia Germination ◽  
Germination Speed ◽  
Speed Parameter ◽  
Mcf 7

Previously, our study reported that β-(1→3,1→6)-ᴅ-glucans produced by endophytes Diaporthe sp. G27-60 and G65-65 (GenBank accession codes JF766998 and JF767007, respectively) are promising antiproliferative agents against human breast carcinoma (MCF-7) and hepatocellular carcinoma (HepG2-C3A) cells. However, effects of Diaporthe exopolysaccharides on eukaryotic healthy cells are not described in current literature. The fungus Metarhizium anisopliae has been employed as teste-system to evaluate the toxicity of pharmaceutical and agricultural-interest substances, considering, among other parameters, conidia germination speed. The current study aimed to verify the effect of different concentrations of Diaporthe β-glucans on the germination speed of M. anisopliae. Conidia were incubated with β-glucans treatments (50, 200 and 400 μg/mL) at 28 ºC, sampled throughout 24 h and analyzed by light microscopy. At the end of 24 h of incubation, the amount of germinated conidia was ≈99% for controls and ranged from 97.7 to 98.6% for treatments. Bayesian analysis indicated that Diaporthe glucans had no toxicity on M. anisopliae and the curve of germination occurred as expected for this fungal strain. Considering the validity of filamentous fungi as model systems, these results are important data about the toxicity of these endophytic EPS on healthy cells and may be associated with previous results obtained for these polymers against tumor cells. Keywords: bayesian analysis, conidia germination, endophytic fungi, exopolysaccharide, fungal model-system.

scholarly journals Survival and mutant production induced by mutagenic agents in Metarhizium anisopliae

1995 ◽  
Vol 52 (3) ◽  
pp. 548-554 ◽  
Author(s):  
V. Kava - Cordeiro ◽  
E.A. Luna - Alves - Lima ◽  
J.L. Azevedo
Keyword(s):  
Gamma Radiation ◽  
Ultraviolet Light ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Nitrous Acid ◽  
Wild Strain ◽  
Wild Type ◽  
Survival Curves ◽  
Auxotrophic Mutants ◽  
Morphological Mutants

A wild strain of Metarhizium anisopliae, an entomopathogenic fungus, was submitted to three mutagenic agents: gamma radiation, ultraviolet light and nitrous acid. Survival curves were obtained and mutants were selected using different mutagenic doses which gave 1 to 5% survival. Morphological and auxotrophic mutants were isolated. Morphological mutants were grouped in a class with yellow conidia and other with pale vinaceous conidia as opposed to the green wild type conidia. Auxotrophic mutants had requirements for vitamin and aminoacid biosynthesis. More than 58% of the total auxotrophk mutants required proline/aipnine. Gamma radiation showed to be the most efficient mutagenic agent giving 0.2% of auxotrophk mutants followed by ultraviolet light (0.12%) and nitrous acid (0.06%).The conidial colour and auxotrophk mutants isolated until now from M. anisopliae were reviewed.

DEMONSTRATION OF A PARASEXUAL CYCLE IN THE ENTOMOPATHOGENIC FUNGUS METARHIZIUM ANISOPLIAE

1980 ◽  
Vol 22 (2) ◽  
pp. 309-314 ◽  
Author(s):  
K. Al-Aidroos
Keyword(s):  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Parasexual Cycle

In an attempt to demonstrate the existence of a parasexual cycle in the imperfect entomopathogenic fungus, Metarhizium anisopliae (Metschnikoff) Sorokin, doubly- and triply-marked strains were forced to form heterokaryons. All heterokaryons produced diploid spores, two of which yielded putative recombinants.

Studies of the inheritance of virulence in the entomopathogenic fungus Metarhizium anisopliae

1985 ◽  
Vol 46 (1) ◽  
pp. 20-25 ◽  
Author(s):  
G. Riba ◽  
J.L. de Azevedo ◽  
C. Messias ◽  
W. Dias da Silveira ◽  
R. Tuveson
Keyword(s):  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus

The effects of isothiocyanates on the growth of the entomopathogenic fungus Metarhizium anisopliae and its infection of the mustard beetle

1999 ◽  
Vol 103 (8) ◽  
pp. 974-980 ◽  
Author(s):  
E.N. Inyang ◽  
T.M. Butt ◽  
K.J. Doughty ◽  
A.D. Todd ◽  
S. Archer
Keyword(s):  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus

scholarly journals Screening for attractants compatible with entomopathogenic fungus Metarhizium anisopliae for use in thrips management

2016 ◽  
Vol 15 (17) ◽  
pp. 714-721 ◽  
Author(s):  
Kupesa Mfuti David ◽  
Subramanian Sevgan ◽  
Niassy Saliou ◽  
Salifu Daisy ◽  
du Plessis Hannalene ◽  
...  
Keyword(s):  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus
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