scholarly journals Evaluation of rough lemon (Citrus jambhiri Lush.) as rootstock for salinity tolerance at seedling stage under in vitro conditions

2013 ◽  
Vol 12 (44) ◽  
pp. 6267-6275 ◽  
Author(s):  
K Sharma L ◽  
Kaushal Manisha ◽  
Kaur Bali Sukhwinder ◽  
P Choudhary O
Keyword(s):  
Salinity Tolerance ◽  
Seedling Stage ◽  
Citrus Jambhiri ◽  
Rough Lemon

scholarly journals In vitro plant regeneration in rough lemon (Citrus jambhiri Lush.) through epicotyl segments by direct shoot organogenesis

2016 ◽  
Vol 8 (2) ◽  
pp. 724-729
Author(s):  
Sukhjit Kaur
Keyword(s):  
In Vitro Regeneration ◽  
Growth Hormones ◽  
Garden Soil ◽  
Direct Regeneration ◽  
Ms Medium ◽  
Citrus Jambhiri ◽  
Rough Lemon ◽  
Induction Frequency ◽  
Bud Induction

The effect of Murashige and Skoog (MS) medium supplemented with various concentrations and combinations of growth hormones on direct regeneration from one month old epicotyl segments of in vitro grown rough lemon (Citrus jambhiri Lush.) seedlings was studied. The earliest bud induction in 7.5 days, highest bud induction frequency (98.50%), percent regeneration(90.53) were obtained on MS medium supplemented with 6-Benzylaminopurine (BAP) (1mglit-1) with an average number of 12.50 buds per explants. The epicotyls segments with proliferated buds were transferred to elongation media in order to improve the recovery of normal shoots. Maximum number of elongated shoots (8.50) was obtained on MS medium having BAP (0.5mglit-1) + Gibberellic Acid (GA3)(1.0 mglit-1).These elongated shoots were then rooted on MS medium containing Indole-3-butyric acid (IBA) (0.1mglit-1) + Indole-3-aceticacid(IAA)(0.5mglit-1) with highest rooting percentage(96%) and root number(5.0). Early (10.10 days) rooting was observed in MS medium supplemented with NAA1.0 mglit-1 + IBA0.5 mglit-1.The plantlet survival was 98.52%, when plantlets were transferred to plastic pots containing a mixture of garden soil and vermiculite (1:1). The hardened plants were successfully established in the soil. The present study developed protocol which can be reliably used for in vitro regeneration of rough lemon and for gene transfer studies in rough lemon, especially to induce salinity and Phytophthora tolerance.

scholarly journals In vitro plant regeneration in rough lemon (Citrus jambhiri L.)

2021 ◽  
pp. 1
Author(s):  
Ashrafi Papry ◽  
Sayeda Sultana ◽  
Goutam Deb ◽  
Mohammed Bhuiyan
Keyword(s):  
Plant Regeneration ◽  
In Vitro Plant Regeneration ◽  
Citrus Jambhiri ◽  
Rough Lemon ◽  
In Vitro Plant

scholarly journals Salinity tolerance at seedling stage for rice genotypes: In vitro analysis

2017 ◽  
Vol 5 (4) ◽  
pp. 126-130 ◽  
Author(s):  
Raghad S. Mouhamad ◽  
◽  
Lamiaa A. Mutlag ◽  
Ameerah H. Atiyah ◽  
Ibrahim B. Razaq ◽  
...  
Keyword(s):  
Salinity Tolerance ◽  
Seedling Stage ◽  
Rice Genotypes ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals Effect of mutagens on regeneration and growth of in vitro grown epicotyl segments of rough lemon seedlings (Citrus jambhiri Lush.)

2015 ◽  
Vol 7 (1) ◽  
pp. 459-465 ◽  
Author(s):  
Sukhjit Kaur
Keyword(s):  
Gamma Radiation ◽  
Primary Root ◽  
Methane Sulfonate ◽  
Internodal Length ◽  
Citrus Jambhiri ◽  
Rough Lemon ◽  
Number Of Leaves ◽  
Developmental Characteristics ◽  
Secondary Roots

The present study on the effect of mutagens on regeneration and growth of in vitro grown epicotyls segments of rough lemon seedlings (Citrus jambhiri Lush.) was carried out during the years 2009- 2010 and 2010- 2011 in the Tissue Culture Laboratory, Department of Fruit Science, Punjab Agricultural University, Ludhiana. Developmental characteristics of the in vitro grown epicotyls segments on regeneration media were treated employing gamma radiation in Gray(Gy) at 0, 5,10,15, 20,25,30,35,40 and 45 Gy; the alkylating agent ethyl methane sulfonate (EMS) and methyl methane sulfonate (MMS) each at 0, 0.1, 0.2, 0.3, 0.4,0.5 and 0.6% (v/v) were evaluated. Epicotyl segments from one month old in vitro grown seedling were cultured in regeneration medium (MS+BAP 1.0 mglit-1) under controlled laboratory conditions (25±2⁰ C, 16 hr photoperiod, 2000 lux light). LD50, the dose required to kill half of the tested population corresponded to 35Gy for gamma radiation, 0.3% each for EMS and MMS treatments. Number of days taken for regeneration increased with increasing dose of gamma irradiation, EMS and MMS. Percent regeneration, number of buds, number of shoots, shoot length, number of leaves, internodal length, primary root length and number of secondary roots decreased with increasing dose of gamma radiation , EMS and MMS. The study would be beneficial to induce desirable variations in plant growth characteristics of rough lemon by the use of mutagens treatment.

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