scholarly journals Co-feeding strategy to enhance phytase production in Pichia pastoris

2014 ◽  
Vol 13 (21) ◽  
pp. 2181-2187 ◽  
Author(s):  
Jean Bernard Ndayambaje, ◽  
Meenakshisundaram S.
Keyword(s):  
Pichia Pastoris ◽  
Feeding Strategy ◽  
Phytase Production

Medium Optimization for the Production of Phytase by Recombinant Pichia pastoris Grown on Glycerol

2011 ◽  
Vol 9 (1) ◽  
Author(s):  
Min Liu ◽  
Gabriel Potvin ◽  
Yiru Gan ◽  
Zhanbin Huang ◽  
Zisheng Zhang
Keyword(s):  
Pichia Pastoris ◽  
Phytase Production ◽  
Medium Components ◽  
Gap Promoter ◽  
Recombinant Pichia Pastoris ◽  
Burman Design ◽  
Statistical Designs ◽  
Plackett Burman Design ◽  
Central Composite ◽  
Three Components

Based on statistical designs, minimal salts medium, commonly used for yeast cultivation, was optimized to maximize GAP promoter-mediated phytase production by recombinant Pichia pastoris grown on glycerol. A Plackett-Burman design was followed to screen medium components to determine those that significantly affected phytase production. Of the 8 components studied, the concentrations of K2SO4, CaSO4•2H2O and MgSO4•7H2O were identified as having a significant effect. These three components were subsequently optimized by response surface methodology using a central composite design. The optimal concentrations of the three components, leading to a maximal extracellular phytase activity of 161.64 U/ml, were K2SO4 13.25g/l, CaSO4•2H2O 1.03g/l and MgSO4•7H2O 17.94g/l. The activity measured in cultures using optimized growth medium is significantly higher than the 73.31 U/ml measured in cultures using standard minimal salts media. The theoretical phytase yields predicted by the developed model were very close to experimentally obtained values.

A novel feeding strategy during the production phase for enhancing the enzymatic synthesis of S-adenosyl-l-methionine by methylotrophic Pichia pastoris

2007 ◽  
Vol 40 (4) ◽  
pp. 669-674 ◽  
Author(s):  
Xiao-Qing Hu ◽  
Ju Chu ◽  
Si-Liang Zhang ◽  
Ying-Ping Zhuang ◽  
Yong-Hong Wang ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Enzymatic Synthesis ◽  
Feeding Strategy ◽  
Production Phase

scholarly journals Engineering the expression system for Komagataella phaffii (Pichia pastoris): an attempt to develop a methanol-free expression system

2019 ◽  
Vol 19 (6) ◽  
Author(s):  
Shinobu Takagi ◽  
Noriko Tsutsumi ◽  
Yuji Terui ◽  
XiangYu Kong ◽  
Hiroya Yurimoto ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Expression System ◽  
Constitutive Expression ◽  
Free Expression ◽  
Aox1 Promoter ◽  
Phytase Production ◽  
Model Case ◽  
Komagataella Phaffii ◽  
Gap Promoter ◽  
Important Region

ABSTRACT The construction of a methanol-free expression system of Komagataella phaffii (Pichia pastoris) was attempted by engineering a strong methanol-inducible DAS1 promoter using Citrobacter braakii phytase production as a model case. Constitutive expression of KpTRM1, formerly PRM1—a positive transcription regulator for methanol-utilization (MUT) genes of K. phaffii,was demonstrated to produce phytase without addition of methanol, especially when a DAS1 promoter was used but not an AOX1 promoter. Another positive regulator, Mxr1p, did not have the same effect on the DAS1 promoter, while it was more effective than KpTrmp1 on the AOX1 promoter. Removing a potential upstream repression sequence (URS) and multiplying UAS1DAS1 in the DAS1 promoter significantly enhanced the yield of C. braakii phytase with methanol-feeding, which surpassed the native AOX1 promoter by 80%. However, multiplying UAS1DAS1 did not affect the yield of methanol-free expression by constitutive KpTrm1p. Another important region to enhance the effect of KpTrm1p under a methanol-free condition was identified in the DAS1 promoter, and was termed ESPDAS1. Nevertheless, methanol-free phytase production using an engineered DAS1 promoter outperformed phytase production with the GAP promoter by 25%. Difference in regulation by known transcription factors on the AOX1 promoter and the DAS1 promoter was also illustrated.

Phytase production by fermentation of recombinant Pichia pastoris in monosodium glutamate wastewater

2009 ◽  
Vol 25 (9) ◽  
pp. 1643-1649 ◽  
Author(s):  
Yingguo Bai ◽  
Peilong Yang ◽  
Yaru Wang ◽  
Pengjun Shi ◽  
Huiying Luo ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Monosodium Glutamate ◽  
Phytase Production ◽  
Recombinant Pichia Pastoris
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