scholarly journals Genetic characterization of fin fish species from the Warri River at Ubeji, Niger Delta, Nigeria

2014 ◽  
Vol 13 (27) ◽  
pp. 2689-2695
Author(s):  
M. C. Asagbra, ◽  
A. S. Adebayo, ◽  
O. A. Ugwumba, ◽  
A. A. A. Ugwumba, ◽  
C. I. Anumudu,
Keyword(s):  
Fish Species ◽  
Niger Delta ◽  
Genetic Characterization

scholarly journals The 16S and COI Mitochondrial DNA Nucleotide Composition of Stripped Snakehead (Channa striata Bloch,1793) from Lake Sentani, Jayapura, Papua

2019 ◽  
Vol 20 (2) ◽  
pp. 57
Author(s):  
Christine Bawaeda Sitandung Kombong ◽  
Tuty Arisuryanti
Keyword(s):  
Mitochondrial Dna ◽  
Fish Species ◽  
Nutritional Value ◽  
Genetic Characterization ◽  
Mitochondrial Gene ◽  
Nucleotide Composition ◽  
Omega 3 ◽  
Channa Striata ◽  
Pcr Method

Stripped snakehead (Channa striata Bloch,1793) has potency to be developed in Indonesia due to high nutritional value including omega 3 which is useful for children growth. However, study on composition of mitochondrial DNA nucleotide as a part of study on genetic characterization of stripped snakehead fish in Indonesia is poorlyunderstood. Genetic characterization of stripped snakehead is important to obtain data information that can be applied for breeding and conservation program. Therefore, the objective of this study was to determine the 16S and COI mitochondrial DNA nucleotide of stripped snakehead collected from Lake Sentani, Jayapura, Papua. Method used in this research is PCR method using primer 16Sar and 16Sbr for 16S mitochondrial gene, and FishF2 and FishR2 for COI mitochondrial gene. The results showed that composition of 16S mtDNA nucleotide from 616 bp was T=21.92%, C=25.49%, A=30.19%, dan G=22.40%, whereas composition of A+T=52.11% and G+C=47.89%. In addition, composition of COI mtDNA nucleotide from 705 bp was T=29.93%, C=28.65%, A=24.26%, and G=17.16% while composition of A+T=54.48% and G+C=45.52%. Data of 16S and COI mitochondrial DNA nucleotide composition of the stripped snakehead collected from Lake Sentani gained in this study are to be expected to complete genetic information of the fish species which is useful to improve breeding program of this fish species in the future.

scholarly journals Morphology and Molecular Studies of Contracaecum Larvae (Nematoda: Anisakidae) in Some Fish species from Sulaimani Province, Kurdistan Region, Iraq

2021 ◽  
Vol 34 (1) ◽  
pp. 93-110
Author(s):  
Younis S. Abdullah ◽  
Shamall M. A. Abdullah ◽  
Ridha H. Hussein
Keyword(s):  
Fish Species ◽  
Larval Stage ◽  
Genetic Characterization ◽  
Kurdistan Region ◽  
Chain Reaction ◽  
Contracaecum Rudolphii ◽  
The Family ◽  
Polymerase Chain ◽  
Paratenic Hosts

Contracaecum is a genus of nematodes belonging to the family Anisakidae, that parasitise many fishes which act as an intermediate or paratenic hosts, while the piscivorous birds and mammals are definitive hosts. A total of 44 third larval stage of Contracaecum were collected from 13 infected freshwater fishes belonging to five different species in different water bodies in Sulaimani Province, Kurdistan Region, Iraq from January to the end of December 2018. In this investigation, 966 fishes were collected including six species of Nemacheilidae, five species of Cyprinidae, three species of Leuciscidae, two species of Xenocyprididae, one species of each of Bagridae, Heteropneustidae, Mastacembelidae, Mugilidae, Siluridae and Sisoridae. This study revealed that five fish species (Cyprinus carpio, Luciobarbus barbulus, L. esocinus, L. xanthopterus and Mastacembelus mastacembelus) were infected with Contracaecum larvae with the prevalence of 2.05%, 0.92%, 1.92%, 19.35% and 1.06%, respectively. The Contracaecum larvae were morphologically studied by compound light microscope and the molecular analyses was done by amplification, sequencing and comparing different gene loci (ITS1, ITS2 and COX2) of isolated third larval stage of Contracaecum. The ITS1, ITS2 and COX2 were amplified by polymerase chain reaction (PCR) and sequenced. The sequences of ITS1, ITS2 and COX2 reveal that all Contracaecum larvae from all infected fishes represented exactly one species (Contracaecum rudolphii B) based on compering and identity percentage in Gene Bank database. Phylogenetic analysis of the genotype (for ITS1) was described. The genetic characterization of the Contracaecum larvae in the present study is available in the GenBank database and they were deposited in GenBank and their accession numbers were demonstrated.

Histopathological and genetic characterization of colorectal mucinous carcinoma

2001 ◽  
Vol 120 (5) ◽  
pp. A166-A166
Author(s):  
S FUJII ◽  
T KUSAKA ◽  
T KAIHARA ◽  
Y UEDA ◽  
T CHIBA ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Mucinous Carcinoma

Genetic characterization of high hyperdiploidy in childhood acute lymphoblastic leukemia

2009 ◽  
Vol 221 (03) ◽  
Author(s):  
R Vagkopoulou ◽  
C Eckert ◽  
U Ungethüm ◽  
G Körner ◽  
M Stanulla ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Genetic Characterization ◽  
Lymphoblastic Leukemia ◽  
Childhood Acute Lymphoblastic Leukemia

TBE in Sweden

2020 ◽  
Keyword(s):  
Genetic Characterization ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Second Phase ◽  
Serum Samples ◽  
Tick Borne Encephalitis ◽  
Specific Immunoglobulin ◽  
Tick Borne Encephalitis Virus ◽  
First Time

Tick-borne encephalitis virus (TBEV) was isolated for the first time in Sweden in 1958 (from ticks and from 1 tick-borne encephalitis [TBE] patient).1 In 2003, Haglund and colleagues reported the isolation and antigenic and genetic characterization of 14 TBEV strains from Swedish patients (samples collected 1991–1994).2 The first serum sample, from which TBEV was isolated, was obtained 2–10 days after onset of disease and found to be negative for anti-TBEV immunoglobulin M (IgM) by enzyme-linked immunosorbent assay (ELISA), whereas TBEV-specific IgM (and TBEV-specific immunoglobulin G/cerebrospinal fluid [IgG/CSF] activity) was demonstrated in later serum samples taken during the second phase of the disease.

Clinical and genetic characterization of two cases of central hypogonadism in Klinefelter syndrome

2020 ◽  
Author(s):  
Rita Indirli ◽  
Biagio Cangiano ◽  
Eriselda Profka ◽  
Elena Castellano ◽  
Giovanni Goggi ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Klinefelter Syndrome
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