Introgression of bacterial blight (BB) resistance genes Xa7 and Xa21 into popular restorer line and their hybrids by molecular marker-assisted backcross (MABC) selection scheme

2012 ◽  
Vol 11 (33) ◽  
Author(s):  
Junying Xu
Keyword(s):  
Molecular Marker ◽  
Resistance Genes ◽  
Bacterial Blight ◽  
Restorer Line ◽  
Selection Scheme

Marker-assisted pyramiding of bacterial blight and gall midge resistance genes into RPHR-1005, the restorer line of the popular rice hybrid DRRH-3

2017 ◽  
Vol 37 (7) ◽  
Author(s):  
V. Abhilash Kumar ◽  
C. H. Balachiranjeevi ◽  
S. Bhaskar Naik ◽  
G. Rekha ◽  
R. Rambabu ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Bacterial Blight ◽  
Gall Midge ◽  
Restorer Line ◽  
Rice Hybrid

Pyramiding Xa21, Bph14, and Bph15 genes into the elite restorer line Yuehui9113 increases resistance to bacterial blight and the brown planthopper in rice

2019 ◽  
Vol 115 ◽  
pp. 31-39 ◽  
Author(s):  
Chao He ◽  
Youlun Xiao ◽  
Jianghui Yu ◽  
Jinjiang Li ◽  
Qiucheng Meng ◽  
...  
Keyword(s):  
Bacterial Blight ◽  
Brown Planthopper ◽  
Restorer Line

scholarly journals Identification of bacterial blight resistance genes in rice landraces from Yunnan Province, China

2022 ◽  
Author(s):  
Caimei Zhao ◽  
Fuyou Yin ◽  
Ling Chen ◽  
Dingqin Li ◽  
Suqin Xiao ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Bacterial Blight ◽  
Yunnan Province ◽  
Growth And Yield ◽  
Bacterial Disease ◽  
Nucleotide Polymorphisms ◽  
Phenotypic Resistance ◽  
Leaf Clipping ◽  
Rice Landraces ◽  
Rice Improvement

AbstractBacterial blight (BB), a serious bacterial disease caused by pathogen Xanthomonas oryzae pv. oryzae (Xoo) affects rice growth and yield. Yunnan Province is regarded as a center of rice diversity in China and indeed the world, and has abundant rice landrace resources, which may offer prospective candidate donors in rice improvement and breeding. In this study, a set of 200 rice landraces were evaluated to determine their resistance to 10 pathogenic Xoo strains resistance by the leaf-clipping method. The results indicated that the tested rice landraces had different resistance levels against different Xoo strains. Multiple comparisons showed that the Xoo strain PXO99 was virulent to the tested rice landraces. Sixty-six rice landraces conferred resistance against at least one Xoo strain. These resistant rice landraces screened were then performed the presence of 14 cloned BB resistance genes by closely linked molecular markers and designed specific primers. The results showed that none of these resistant accessions contained xa13, Xa21, Xa27, and Xa45(t) homologous fragments, while 9, 24, 4, 7, 9, 15, 1, 5, 4 and 27 accessions contained Xa1, Xa2/Xa31(t), Xa14, Xa3/Xa26, Xa4, xa5, Xa7, Xa10, Xa23 and xa25 homologous fragments, respectively. Sequence analysis further revealed that nucleotide variations around functional nucleotide polymorphisms region were observed within these accessions containing the Xa1, Xa2/Xa31(t), Xa14, Xa3/Xa26, Xa4, xa5, Xa10, Xa23 and xa25 homologous fragments. These results along with phenotypic resistance spectrum supported that these accessions carried nine resistance homologous genes. Only one accession (Qishanggu_Wenshan) carried the Xa7 resistance gene. We also found that some resistant rice landraces, especially Xilandigu_Baoshan, and Laoyaling_Lincang without the above resistance genes, which mediated broad spectrum resistance to multiple Xoo strains, were identified as potential sources for breeding rice lines resistance to BB.

scholarly journals Genetic diversity analysis in indigenous rice (Oryza sativa L.) germplasm for Bacterial Leaf Blight (Xanthomonas oryzae pv. Oryzae) using resistance genes linked markers

2021 ◽  
Author(s):  
Khannetah K. R . ◽  
Ramchander S. ◽  
Andrew Peter Leon M. T ◽  
Shobha D. ◽  
Saravanan S. ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Resistance Genes ◽  
Bacterial Blight ◽  
Rice Yield ◽  
Oryza Sativa L ◽  
Molecular Data ◽  
Lesion Length ◽  
R Genes ◽  
Leaf Clipping ◽  
Moderately Resistant

Abstract Among rice ( Oryza sativa L.) diseases, bacterial blight (BB), caused by Xanthomonas oryza pv. Oryzae , plays a pivotal role in decreasing rice yield. BB is reported to be the most serious constraint to improving rice yield. The present investigation assessed the potentiality of bacterial blight resistance and molecular characterization of 100 rice accessions for four major BB resistance genes, viz., Xa4, xa5, xa13 and Xa21 . Disease screening was carried out under glasshouse conditions using a BB culture isolated from BB-infected rice fields through the leaf clipping method. Analyses of 13 BB resistance genes linked with polymorphic microsatellites markers indicated the presence of single-, two-, three- and four-gene combinations of xa5, xa13, Xa4 and Xa21 . We found four accessions to be resistant; 34 accessions to be moderately resistant; 49 accessions to be moderately susceptible and 13 accessions to be susceptible. Among the resistant lines, IR12L110, Namcheonbyeo, Dhalaheera and SahbhagiDhan recorded a minimum lesion length of 3.7cm, 4.2cm, 4.67cm and 8.3 cm, respectively. Phylogenetic tree, constructed using molecular data, grouped the rice germplasm into four major clusters. R genes xa 5 and Xa4 contributed 14 positive compatible R genes, each belonging to 28 germplasm for BB resistance. The potential genetic resources identified as resistant to BB can be used as donors for the improvement of rice BB resistance in rice breeding programs.

Sign in / Sign up

Export Citation Format

Share Document