scholarly journals Development of an indirect enzyme-linked immunosorbent assay (ELISA) assay based on a recombinant truncated VP2 (tVP2) protein for the detection of canine parvovirus antibodies

2012 ◽  
Vol 11 (93) ◽  
pp. 16034-16039 ◽  
Author(s):  
Shi Lijun ◽  
Wang Jing ◽  
Wang Peng ◽  
Li Gang ◽  
Gong Miaomiao ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Canine Parvovirus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Assay

Quantification of human apolipoprotein A-IV by "sandwich"-type enzyme-linked immunosorbent assay.

1988 ◽  
Vol 34 (4) ◽  
pp. 739-743 ◽  
Author(s):  
M Rosseneu ◽  
G Michiels ◽  
W De Keersgieter ◽  
J Bury ◽  
J P De Slypere ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Assay ◽  
Chromatographic Fractionation ◽  
Human Apolipoprotein ◽  
Apolipoprotein A ◽  
Sandwich Type ◽  
Antibody Conjugate ◽  
The Mean

Abstract A specific and sensitive "sandwich"-type enzyme-linked immunosorbent assay (ELISA) has been developed for quantifying human apo A-IV. Using apo A-IV immunosorbent columns, we isolated monospecific anti-apo A-IV antibodies for coating the ELISA plates and for preparing peroxidase-antibody conjugate. The assay can detect as little as 0.20 ng of apo A-IV, with mean intra- and interassay CVs of 3.6% and 8.2%, respectively. The apoA-IV concentrations in normolipemic and hyperlipemic plasma were unaffected by either delipidation or treatment with detergents or urea. To validate the ELISA assay we compared it with an immunoelectrophoretic technique. ApoA-IV concentrations in plasma from normo- and dyslipemic subjects compared well by the two assays (r = 0.89). The mean apo A-IV concentration, measured by ELISA in plasma from 50 normolipemic subjects, was 143 (SD 52) mg/L; values for dyslipemic subjects were not significantly different. We also used this new assay to monitor apo A-IV profiles of normolipemic and hypertriglyceridemic plasma after chromatographic fractionation.

Chemical synthesis of GDP-fucose analogs and their utilization by the Lewis *A(1 → 4) fucosyltransferase

1990 ◽  
Vol 68 (7) ◽  
pp. 1063-1071 ◽  
Author(s):  
Uday B. Gokhale ◽  
Ole Hindsgaul ◽  
Monica M. Palcic
Keyword(s):  
Nmr Spectroscopy ◽  
Chemical Synthesis ◽  
Human Milk ◽  
Immunosorbent Assay ◽  
Spectrophotometric Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Assay ◽  
Oligosaccharide Synthesis ◽  
H Nmr ◽  
Nucleotide Analog

Chemical syntheses are reported for GDP-fucose (5), GDP-3-deoxy-fucose (6), and GDP-arabinose (7), the demethyl analog of 5. All three sugar nucleotides were found to act as donor substrates for an α(1 → 4) fucosyltransferase isolated from human milk when *BDGal(1 → 3)*BDGlcNAc-O(CH2)8COOMe (1) was used as the acceptor. The rate of transfer of sugar residues to 1 was measured using a coupled spectrophotometric assay and was found to be 100% (5), 2.3% (6), and 5.9% (7). The product Lea-active oligosaccharide analogs were identified by both an enzyme-linked immunosorbent assay (ELISA) and by 1H NMR spectroscopy. Keywords: glycosyltransferase, oligosaccharide synthesis, sugar-nucleotide analog, ELISA assay, fucosyltransferase.

scholarly journals Detection of Campylobacter jejuni and Campylobacter coli in Environmental Waters by PCR Enzyme-Linked Immunosorbent Assay

2002 ◽  
Vol 68 (3) ◽  
pp. 1319-1324 ◽  
Author(s):  
A. D. Sails ◽  
F. J. Bolton ◽  
A. J. Fox ◽  
D. R. A. Wareing ◽  
D. L. A. Greenway
Keyword(s):  
Campylobacter Jejuni ◽  
Immunosorbent Assay ◽  
Water Samples ◽  
Enrichment Culture ◽  
Environmental Water ◽  
Environmental Water Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Campylobacter Coli ◽  
Elisa Assay ◽  
Bacterial Cells

ABSTRACT A PCR enzyme-linked immunosorbent assay (ELISA) assay was applied to the detection of Campylobacter jejuni and Campylobacter coli in environmental water samples after enrichment culture. Bacterial cells were concentrated from 69 environmental water samples by using filtration, and the filtrates were cultured in Campylobacter blood-free broth. After enrichment culture, DNA was extracted from the samples by using a rapid-boiling method, and the DNA extracts were used as a template in a PCR ELISA assay. A total of 51 samples were positive by either PCR ELISA or culture; of these, 43 were found to be positive by PCR ELISA and 43 were found to be positive by culture. Overall, including positive and negative results, 59 samples were concordant in both methods. Several samples were positive in the PCR ELISA assay but were culture negative; therefore, this assay may be able to detect sublethally damaged or viable nonculturable forms of campylobacters. The method is rapid and sensitive, and it significantly reduces the time needed for the detection of these important pathogens by 2 to 3 days.

scholarly journals Detection of Antibody to Canine Parvovirus by Enzyme-linked Immunosorbent Assay

1994 ◽  
Vol 47 (5) ◽  
pp. 335-338
Author(s):  
Motonobu HARA ◽  
Masafumi FUKUYAMA ◽  
Seigo KISHIKAWA ◽  
Sizuo YAMAMOTO ◽  
Teruo IKEDA ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Canine Parvovirus ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Assessment of Maternal Antibody Decay and Response to Canine Parvovirus Vaccination Using a Clinic-Based Enzyme-Linked Immunosorbent Assay

1996 ◽  
Vol 8 (4) ◽  
pp. 427-432 ◽  
Author(s):  
Trevor Waner ◽  
Ami Naveh ◽  
Ilana Wudovsky ◽  
Leland E. Carmichael
Keyword(s):  
Immunosorbent Assay ◽  
Cost Effective ◽  
Canine Parvovirus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Test Results ◽  
Elisa Method ◽  
Vaccination Response ◽  
Critical Phase ◽  
Effective Program ◽  
Antibody Determination

Interference caused by maternal antibodies is considered a major cause of canine parvovirus (CPV) vaccination failure. In this study, an immunoblot clinic-based enzyme-linked immunosorbent assay (ELISA) method was used to detect CPV antibodies in sera of pregnant bitches and their offspring to study the response of pups to vaccination. With a easily accessible procedure for CPV antibody determination, the veterinarian should be able to gauge the response of pups after vaccination. The validity of the technique was tested in parallel against the standard hemagglutination inhibition (HI) test. Results of the ELISA were correlated with those of the standard HI method for quantification of CPV antibodies. With the ELISA, successfully immunized pups were identified, allowing for a more reliable and cost-effective program of vaccination. This simple clinic-based test could be used for the assessment of vaccination status of pups during the critical phase of 6 to about 16 weeks of age. This study is the first in which vaccination response to CPV in pups was followed, using a clinic-based ELISA for CPV antibody monitoring.

Survey of Total Tetrahydrophthalimide in Baby Foods Using Both Enzyme-Linked Immunosorbent Assay and Gas Chromatography/Mass Spectrometry: A Comparative Study

1993 ◽  
Vol 76 (6) ◽  
pp. 1225-1229 ◽  
Author(s):  
Jupiter M Yeung ◽  
W Harvey Newsome
Keyword(s):  
Immunosorbent Assay ◽  
Food Samples ◽  
Baby Food ◽  
Gas Chromatography Mass Spectrometry ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Assay ◽  
Elisa Method ◽  
Baby Foods ◽  
Fruit Samples ◽  
Chromatographic Mass

Abstract An enzyme-linked immunosorbent assay (ELISA) method was compared with a gas chromatographic/ mass spectrometric (GC/MS) method for determining the concentration (in parts per million) of the combination of captan and its degradation product tetrahydrophthalimide (THPI) in 13 fruit samples and in a survey of baby foods. Ninety baby foods (49 fruits, 28 juices, and 13 vegetables) from 2 different suppliers were sampled. All captan in the samples was converted to THPI before each analysis. None of the samples contained a concentration of combined captan and THPI that violated the maximum residue limit of 5.0 ppm. Eight samples of baby food tested positive for THPI at levels ranging from 0.019-0.041 ppm by the GC/MS method, whereas 20 samples tested positive in the ELISA assay. All samples that tested positive with the GC/MS method also tested positive with the ELISA method. Thirteen percent of the baby food samples tested false positive with the ELISA method. The ELISA assay also gave higher values than the GC/MS method. The ELISA method can be effectively used as a primary screening tool to select samples testing positive for THPI. The concentration of THPI in these samples can then be verified using the GC/MS method.

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