Optimization of lipid production in the oleaginous bacterium Rhodococcus erythropolis growing on glycerol as the sole carbon source

2012 ◽  
Vol 11 (79) ◽  
Author(s):  
Salinee Sriwongchai
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Lipid Production ◽  
Rhodococcus Erythropolis

Biosurfactant production by Rhodococcus erythropolis grown on glycerol as sole carbon source

1996 ◽  
Vol 131 (1-3) ◽  
pp. 880-886
Author(s):  
Elisa M. P. Ciapina ◽  
Walber C. Melo ◽  
Lidia M. M. Santa Anna ◽  
Alexandre S. Santos ◽  
Denise M. G. Freire ◽  
...  
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Rhodococcus Erythropolis ◽  
Biosurfactant Production

Biosurfactant Production by Rhodococcus erythropolis Grown on Glycerol As Sole Carbon Source

2006 ◽  
pp. 880-886 ◽  
Author(s):  
Elisa M. P. Ciapina ◽  
Walber C. Melo ◽  
Lidia M. M. Santa Anna ◽  
Alexandre S. Santos ◽  
Denise M. G. Freire ◽  
...  
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Rhodococcus Erythropolis ◽  
Biosurfactant Production

scholarly journals Biodegradation of the Organic Disulfide 4,4′-Dithiodibutyric Acid by Rhodococcus spp.

2015 ◽  
Vol 81 (24) ◽  
pp. 8294-8306 ◽  
Author(s):  
Heba Khairy ◽  
Jan Hendrik Wübbeler ◽  
Alexander Steinbüchel
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Rhodococcus Erythropolis ◽  
Model Organism ◽  
Hypothetical Protein ◽  
Gene Encoding ◽  
Content Type ◽  
Gene Coding ◽  
Initial Cleavage ◽  
Genes Encoding

ABSTRACTFourRhodococcusspp. exhibited the ability to use 4,4′-dithiodibutyric acid (DTDB) as a sole carbon source for growth. The most important step for the production of a novel polythioester (PTE) using DTDB as a precursor substrate is the initial cleavage of DTDB. Thus, identification of the enzyme responsible for this step was mandatory. BecauseRhodococcus erythropolisstrain MI2 serves as a model organism for elucidation of the biodegradation of DTDB, it was used to identify the genes encoding the enzymes involved in DTDB utilization. To identify these genes, transposon mutagenesis ofR. erythropolisMI2 was carried out using transposon pTNR-TA. Among 3,261 mutants screened, 8 showed no growth with DTDB as the sole carbon source. In five mutants, the insertion locus was mapped either within a gene coding for a polysaccharide deacetyltransferase, a putative ATPase, or an acetyl coenzyme A transferase, 1 bp upstream of a gene coding for a putative methylase, or 176 bp downstream of a gene coding for a putative kinase. In another mutant, the insertion was localized between genes encoding a putative transcriptional regulator of the TetR family (noxR) and an NADH:flavin oxidoreductase (nox). Moreover, in two other mutants, the insertion loci were mapped within a gene encoding a hypothetical protein in the vicinity ofnoxRandnox. The interruption mutant generated,R. erythropolisMI2noxΩtsr, was unable to grow with DTDB as the sole carbon source. Subsequently,noxwas overexpressed and purified, and its activity with DTDB was measured. The specific enzyme activity of Nox amounted to 1.2 ± 0.15 U/mg. Therefore, we propose that Nox is responsible for the initial cleavage of DTDB into 2 molecules of 4-mercaptobutyric acid (4MB).

Use of Crude Glycerol as Sole Carbon Source for Microbial Lipid Production by Oleaginous Yeasts

2016 ◽  
Vol 182 (2) ◽  
pp. 495-510 ◽  
Author(s):  
Li-ping Liu ◽  
Yang Hu ◽  
Wen-yong Lou ◽  
Ning Li ◽  
Hong Wu ◽  
...  
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Crude Glycerol ◽  
Lipid Production ◽  
Microbial Lipid ◽  
Oleaginous Yeasts ◽  
Microbial Lipid Production

Isolation of One Strain for Biodegradation of Diesel Fuel from Sea Water

2013 ◽  
Vol 726-731 ◽  
pp. 2424-2427
Author(s):  
Pei Pei Han ◽  
Ping Zhe Jiang ◽  
Xiang He Liu ◽  
Hong Yi Huang ◽  
Wen Qian Duan ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Carbon Source ◽  
Phylogenetic Tree ◽  
Diesel Fuel ◽  
Sole Carbon Source ◽  
Culture Medium ◽  
Sea Water ◽  
Rhodococcus Erythropolis ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis

Four diesel fuel degrading microorganisms were isolated from soil and sea water from Crude Oil Terminal at Tianjin Port using diesel fuel as sole carbon source, and they all could degrade diesel fuel. The initial diesel fuel concentration in the culture medium was optimized and was 1.5 g/L. The ability of the four strains to degrade diesel was compared by spectrophotometer with the initial diesel concentration at 1.5 g/L. The results showed that the strain C1 had the highest degrading activity, which could degrade 32.59% of diesel in 7 days. The strain C1 was further identified by 16S rDNA sequence analysis as Rhodococcus erythropolis, and the phylogenetic tree of the strain C1 was constructed.

Culture strategies for lipid production using acetic acid as sole carbon source by Rhodosporidium toruloides

2016 ◽  
Vol 206 ◽  
pp. 141-149 ◽  
Author(s):  
Xiang-Feng Huang ◽  
Jia-Nan Liu ◽  
Li-Jun Lu ◽  
Kai-Ming Peng ◽  
Gao-Xiang Yang ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Lipid Production ◽  
Rhodosporidium Toruloides ◽  
Culture Strategies

Biosurfactant Production by Rhodococcus erythropolis Grown on Glycerol As Sole Carbon Source

2006 ◽  
Vol 131 (1-3) ◽  
pp. 880-886 ◽  
Author(s):  
Elisa M. P. Ciapina ◽  
Walber C. Melo ◽  
Lidia M. M. Santa Anna ◽  
Alexandre S. Santos ◽  
Denise M. G. Freire ◽  
...  
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Rhodococcus Erythropolis ◽  
Biosurfactant Production

scholarly journals Pandrug-resistant Pseudomonas sp. expresses New Delhi metallo-β-lactamase-1 and consumes ampicillin as sole carbon source

2020 ◽  
Author(s):  
Vivek Kumar Ranjan ◽  
Shriparna Mukherjee ◽  
Subarna Thakur ◽  
Krutika Gupta ◽  
Ranadhir Chakraborty
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
New Delhi ◽  
Pseudomonas Sp
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