Molecular characterization of two populations of catfish Clarias batrachus L. using random amplified polymorphic DNA (RAPD) markers

2012 ◽  
Vol 11 (77) ◽  
Author(s):  
Mohd. Danish
Keyword(s):  
Molecular Characterization ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Clarias Batrachus ◽  
Two Populations

scholarly journals Development of random amplified polymorphic DNA markers for authentication of Croton tiglium Linn.

2017 ◽  
Vol 6 (3) ◽  
pp. 164-166
Author(s):  
Shweta Vekariya ◽  
◽  
Krushnkumar Taviad ◽  
RN Acharya RN ◽  
CN Harisha ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Rapd Markers ◽  
Ricinus Communis ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
Natural Habitat ◽  
Drug Market ◽  
Croton Tiglium ◽  
Young Leaves

Background: Croton tiglium Linn., commonly known as Jayapala, in Ayurvedic pharmacopoeia, is wellknown for its purgative action. In the herbal raw drug market, seeds of Baliospermum montanum Blume., Ricinus communis Linn. and Croton roxburghii Wall. etc. are sold in the name of Jayapla seeds due to their morphological similarities. Hence their identification through molecular characters is need of the hour. Aim: Present study aims with the molecular characterization of young leaves of C. tiglium Linn. Materials & Methods: Fresh young leaves of C. tiglium Linn. were collected from its natural habitat Udupi, Karnnataka, during January 2017. It was used for molecular characterization and DNA fingerprints, by standard and most convenient Random Amplified Polymorphic DNA (RAPD) markers at Food testing laboratory, Junagadh Agriculture University, Gujarat, India. Results: All the primers gave good band patterns. Primer 5, 6, 8, 11 and 13 showed more number of light and bright bands matching characters with plant. Conclusion: Observed RAPD marker can be used to differentiate genuine as well as adulterated samples. The results may be used for the further research purposes and also required DNA Barcoding studies for further authentication.

scholarly journals Molecular characterization of Cuban endemism Carica cubensis Solms using random amplified polymorphic DNA (RAPD) markers

2010 ◽  
Vol 01 (03) ◽  
pp. 95-101 ◽  
Author(s):  
Jesús Rodríguez ◽  
Pedro Rodríguez ◽  
María E. González ◽  
Pedro Martínez-Gómez
Keyword(s):  
Molecular Characterization ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna

scholarly journals Random Amplification of Polymorphic DNA Analysis for Genetic Characterization of Two Breeds of Dogs, German Shepherd and Japanese Spitz

2013 ◽  
Vol 13 (2) ◽  
pp. 73-78
Author(s):  
Jarina Joshsi ◽  
Lumanti Manandhar ◽  
Patima Shrestha ◽  
Rani Gupta ◽  
Rojlina Manadhar ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Dna Analysis ◽  
Genetic Characterization ◽  
Random Amplified Polymorphic Dna ◽  
Neighbor Joining ◽  
German Shepherd ◽  
Polymorphic Loci ◽  
Random Amplification

Random amplified polymorphic DNA (RAPD) markers were used to study genetic diversity in dog samples belonging to populations of German Shepherd and Japanese Spitz. A total of twelve samples were typed using eight RAPD primers. Out of eight primers, three primers gave result in six individuals of dogs. The phylogenetic tree constructed by the neighbor joining method based on Nei. Original measures revealed highest genetic identity found in German Shepherd as 0.9444 and highest genetic distance as 1.2809. The analysis predicts the number of polymorphic loci as 15 and the percentage of polymorphic loci as 83.3. Nepal Journal of Science and Technology Vol. 13, No. 2 (2012) 73-78 DOI: http://dx.doi.org/10.3126/njst.v13i2.7717

MOLECULAR CHARACTERIZATION OF INTERSPECIFIC HYBRIDS OF SCENTED ROSES USING RAPD MARKERS

2007 ◽  
pp. 175-179
Author(s):  
N. Kaur ◽  
R.K. Sharma ◽  
D. Dhyani ◽  
S. Karthigeyan ◽  
P.S. Ahuja
Keyword(s):  
Molecular Characterization ◽  
Interspecific Hybrids ◽  
Rapd Markers

Molecular Diversity Analysis of Lentil (Lens culinaris Medik.) through RAPD Markers

2012 ◽  
Vol 22 (1) ◽  
pp. 51-58 ◽  
Author(s):  
M.E. Hoque ◽  
M.M. Hasan
Keyword(s):  
Genetic Distance ◽  
Rapd Markers ◽  
Gene Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Molecular Genetic ◽  
Group Method ◽  
Diversity Analysis ◽  
Pair Group ◽  
Molecular Genetic Diversity

Random Amplified Polymorphic DNA (RAPD) markers were used to study the molecular genetic diversity analysis among six BARI released lentil varieties viz. BARI masur-1, BARI masur-2, BARI masur-3, BARI masur-4, BARI masur-5 and BARI masur-6. PCR amplified products were visualized on 1.0% agarose gel and the band for each primer were scored. Ten RAPD markers were used in this study. Out of them 7 primers showed amplification of 53 DNA fragments with 60.37% of them being polymorphic. The highest number of polymorphic loci was noticed in the variety BARI masur-3. The same variety also showed maximum Nei’s gene diversity value (0.0552). The highest Nei’s genetic distance (0.5002) was observed in BARI masur-1 vs. BARI masur-5 whereas, the lowest genetic distance (0.0692) was found in BARI masur-1 vs. BARI masur-2. The unweighted pair group method of arithmetic mean (UPGMA) dendrogram based on Nei’s genetic distance grouped the six cultivars into two main clusters. BARI masur-1, BARI masur-2 and BARI masur-3 were in cluster I and BARI masur-4, BARI masur-5 and BARI masur-6 were in cluster II. The cultivar BARI masur-4 was closest to the cultivar BARI masur-6 with the lowest genetic distance (0.0972) and the highest genetic distance (0.5002) was found between BARI masur-1 and BARI masur-5. The RAPD markers were found to be useful in molecular characterization of lentil varieties which could be utilized by the breeders for the improvement of lentil cultivars. DOI: http://dx.doi.org/10.3329/ptcb.v22i1.11260 Plant Tissue Cult. & Biotech. 22(1): 51-58, 2012 (June)

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