Mechanism of action of hydrogen peroxide in wheat thermotolerance - interaction between antioxidant isoenzymes, proline and cell membrane

2012 ◽  
Vol 11 (78) ◽  
Author(s):  
Ranjeet Ranjan Kumar
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Membrane ◽  
Mechanism Of Action

Requirements of intracellular iron for the mechanism of action of bleomycin and hydrogen peroxide.

1991 ◽  
Vol 43 (2-3) ◽  
pp. 456 ◽  
Author(s):  
Kimette Radtke ◽  
Robert Byrnes ◽  
Frank Lornitzo ◽  
William E. Antholine ◽  
David H. Petering
Keyword(s):  
Hydrogen Peroxide ◽  
Mechanism Of Action ◽  
Intracellular Iron

scholarly journals Studies on In-vitro Anti-inflammatory and Antioxidant Potentials of Annona muricata Leaf Extracts

2020 ◽  
Vol 12 (2) ◽  
pp. 177-183
Author(s):  
Idorenyin Nwaehujor ◽  
Samuel Akande ◽  
Olubunmi Atolani ◽  
Gabriel Olatunji
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Membrane ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Scavenging Activity ◽  
Membrane Stabilization ◽  
Anti Inflammatory ◽  
Proteinase Inhibition

Inflammation has stimulated significant worldwide scientific interest because of its implication in many human diseases. Most inflammations are caused by reactive oxygen species or free radicals. Annona muricataleaf extracts were investigated for their in-vitroantioxidant and anti-inflammatory potentials. Annona muricataleavesweredried at room temperature, blended using a mill.and extracted with solvents of varying degree of polarities. The solventsused were hexane, ethyl acetate,and ethanol. After sequential extraction, the crude extracts were examined for their in-vitroanti-inflammatory activities on lipoxygenase inhibition, proteinase inhibition, albumin denaturation inhibition,and red blood cell membrane stabilization assays,while the antioxidant activities were examined using DPPH, ABTS and hydrogen peroxide assays. The results showed that the ethanol extract had significantlyhigher albumin denaturation inhibition activity at 500 μg/mL (p < 0.01). The activity of all the extracts on proteinase inhibition decreased with the increase in concentration of the extracts. Indomethacin (standard), ethanol extract,and ethyl acetate extract exhibited a dose dependent increase in lipoxygenase activity. The ethanol extract showed highred blood cell membrane stabilization activity at 500 μg/mL and the activity was comparable with that of the standard (diclofenac). Hydrogen peroxide scavenging activity of the extracts and standard (Vitamin C) were comparable at 20 –100 μg/mL. The ethanol extract showed significantly higher(p < 0.01) DPPH radical scavenging activity compared with other extracts. A similar trend was also observed for ABTS radical scavenging activity. Generally,the ethanol extract exhibited higher anti-inflammatory and antioxidant activities in most of the assays, this could be attributed to the polar compounds present in the extract.

scholarly journals Pathophysiological and pathochemical aspects of the effect of hydrogen peroxide on humans and animals (review)

2021 ◽  
pp. 30-36
Author(s):  
Д.А. Еникеев ◽  
К.О. Кузнецов ◽  
О.А. Еникеев ◽  
Л.Т. Идрисова ◽  
С.А. Еникеева ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Mechanism Of Action ◽  
Antibacterial Agent ◽  
Negative Effects ◽  
Animal Body

В обзоре рассмотрены вопросы частной патофизилогии перекиси водорода (ПВ), особенности её взаимодействия с органами и тканями организма человека и животных. Показано, что ПВ является уникальным веществом, которое может оказывать как положительное, так и отрицательное действие на организм, является важным соединением в сохранении гомеостаза, так как это естественное антибактериальное средство, в том числе в мочевыделительной и пищеварительной системах. Также оценена возможность использования ПВ с целью лечения различных заболеваний, с учетом знаний о патогенезе заболеваний и механизме действия ПВ. The review deals with the issues of special pathophysiology of hydrogen peroxide (HP), and especially with its interaction with organs and tissues of the human and animal body. HP has been shown to be a unique substance that can have both positive and negative effects. As HP is a natural antibacterial agent, it is an important compound in maintaining homeostasis, including homeostasis in the urinary and digestive systems. The possibility of using HP for the treatment of various diseases was also assessed, taking into account knowledge about the pathogenesis of diseases and the mechanism of action of HP.

In vitro Antifungal Activity and Mechanism of Action of Tea Polyphenols and Tea Saponin against Rhizopus stolonifer

2015 ◽  
Vol 25 (4) ◽  
pp. 269-276 ◽  
Author(s):  
Xiaodong Jiang ◽  
Kejue Feng ◽  
Xiaoping Yang
Keyword(s):  
Cell Membrane ◽  
Oxidative Damage ◽  
Mechanism Of Action ◽  
Soluble Sugar ◽  
Tea Polyphenols ◽  
Cell Membrane Permeability ◽  
Dependent Manner ◽  
Rhizopus Stolonifer ◽  
Tea Saponin

The in vitro antifungal activities and mechanism of action of tea polyphenols (TP), tea saponin (TS) and their combination were evaluated against <i>Rhizopus stolonifer</i>. The results showed that both TP and TS inhibited the mycelial growth in a dose-dependent manner, and their combination at the ratio of 7:3 exhibited synergistic antifungal interaction. We also observed that the treatment of TP or TS significantly induced the production of H<sub>2</sub>O<sub>2</sub> and resulted in membrane lipid peroxidation, thus leading to an increase in cell membrane permeability and the leakage of K<sup>+</sup>, soluble protein and soluble sugar. Moreover, combining them for treatment increased the induction of H<sub>2</sub>O<sub>2</sub> production and oxidative damage. Scanning electron microscopic observations also showed the damage to the hyphal cell structure. It was concluded that TP, TS and their combination inhibit the growth of <i>R. stolonifer</i> through the induction of H<sub>2</sub>O<sub>2</sub> production, leading to cell membrane oxidative damage and intracellular constituent leakage. These findings suggest that TP and TS can potentially be used as an alternative to control postharvest fruit diseases caused by <i>R. stolonifer.</i>

scholarly journals Chemical Composition and Mechanism of Vibriocidal Action of Essential Oil from Resin of Protium heptaphyllum

2019 ◽  
Vol 2019 ◽  
pp. 1-6 ◽  
Author(s):  
Josiane Lima Mendes ◽  
Thiago Ferreira de Araújo ◽  
Mário Geraldo de Carvalho ◽  
Francisco Eduardo Aragão Catunda Júnior ◽  
Renata Albuquerque Costa
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Cell Membrane ◽  
Membrane Permeability ◽  
Mechanism Of Action ◽  
Reducing Sugars ◽  
Antibiofilm Activity ◽  
Pharmacological Activities ◽  
Biomass Reduction ◽  
Cell Constituent

Protium heptaphyllum is a plant widely distributed in Brazilian ecosystems that produce a resin which has pharmacological activities. In this study, the chemical composition, antimicrobial and antibiofilm activity, and the possible mechanism of action against the bacterium V. parahaemolyticus of essential oil from P. heptaphyllum (EOPH) were investigated. Twenty-two components were detected in EOPH, and β-phellandrene (60.68%) had the majority. The inhibition halo, MIC, and MBC values were 11 mm, 2 mg/mL, and 8 mg/mL, respectively. Biofilm biomass inhibition and biomass reduction of the preformed biofilm were detected at 4 mg/mL EOPH concentration. The assays of cell constituent release and membrane permeability indicated that EOPH may disrupt the cell membrane, leading to leakage of intracellular constituent as reducing sugars and materials with an absorbance of 260 nm.

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