Role of antibiosis on suppression of bacterial common blight disease in French bean by Paenibacillus polymyxa strain HKA-15

2012 ◽  
Vol 11 (60) ◽  
Author(s):  
Vellaichamy Mageshwaran
Keyword(s):  
Paenibacillus Polymyxa ◽  
French Bean ◽  
Blight Disease

scholarly journals Inoculation of stigma colonizing microbes to apple stigmas alters microbiome structure and reduces the occurrence of fire blight disease

2020 ◽  
Author(s):  
Zhouqi Cui ◽  
Regan B. Huntley ◽  
Neil Schultes ◽  
Blaire Steven ◽  
Quan Zeng
Keyword(s):  
Fire Blight ◽  
Plant Diseases ◽  
Disease Suppression ◽  
Bacterial Strains ◽  
Microbiome Composition ◽  
Microbial Inoculation ◽  
Short Life Span ◽  
Blight Disease ◽  
Different Strains

Flowers secrete nutrient rich exudates that support the growth of an assemblage of microorganisms, including both beneficial and pathogenic members, most of which belong to the phylum Proteobacteria. Given the potential role of the microbiome in plant health, manipulating the microbiome to promote growth of beneficial members holds promise in controlling plant diseases. In this study we inoculated four different bacterial strains that were originally isolated from apple stigmas, alone or in mixtures of increasing complexity, onto apple flowers during bloom. We tested if such treatments would influence fire blight occurrence, a disease caused by Erwinia amylovora, and if we could detect a shift in the structure of the microbiome due to the treatments. We show that various inoculations did influence the occurrence of fire blight, although the level of disease suppression was dependent upon specific bacterial strains. Furthermore, treatments using different strains or strain mixtures predominantly resulted in increased representation of the inoculated strains, suggesting that disease suppression was due to an alteration of the stigma microbiome structure. Compared to treatments with single strains, a Pantoea-Pseudomonas strain mixture produced a homogeneous microbiome structure with less inter-flower variability. Findings from this study suggest the microbiome on the flower stigma can be manipulated through microbial inoculation. Due to flowers’ short life span yet important role in plant disease infection, even a shot-term influence on microbiome composition may result in significant decreases in disease susceptibility.

scholarly journals HIPM Is a Susceptibility Gene of Malus spp.: Reduced Expression Reduces Susceptibility to Erwinia amylovora

2019 ◽  
Vol 32 (2) ◽  
pp. 167-175 ◽  
Author(s):  
Manuela Campa ◽  
Stefano Piazza ◽  
Laura Righetti ◽  
Chang-Sik Oh ◽  
Lorenza Conterno ◽  
...  
Keyword(s):  
Crop Production ◽  
Fire Blight ◽  
Erwinia Amylovora ◽  
Susceptibility Gene ◽  
Bimolecular Fluorescence Complementation ◽  
Interacting Protein ◽  
Blight Disease ◽  
Oxygen Evolving ◽  
Transgenic Apple

Fire blight, a devastating disease caused by the bacterium Erwinia amylovora, is a major threat to apple crop production. To improve our understanding of the fire blight disease and to identify potential strategies to control the pathogen, we studied the apple protein HIPM (for HrpN-interacting protein from Malus spp.), which has previously been identified as interacting with the E. amylovora effector protein HrpN. Transgenic apple plants were generated with reduced HIPM expression, using an RNA interference construct, and were subsequently analyzed for susceptibility to E. amylovora infection. Lines exhibiting a greater than 50% silencing of HIPM expression showed a significant decrease in susceptibility to E. amylovora infection. Indeed, a correlation between HIPM expression and E. amylovora infection was identified, demonstrating the crucial role of HIPM during fire blight disease progression. Furthermore, an apple oxygen-evolving enhancer-like protein (MdOEE) was identified via a yeast two-hybrid screen to interact with HIPM. This result was confirmed with bimolecular fluorescence complementation assays and leads to new hypotheses concerning the response mechanism of the plant to E. amylovora as well as the mechanism of infection of the bacterium. These results suggest that MdOEE and, particularly, HIPM are promising targets for further investigations toward the genetic improvement of apple.

scholarly journals Role of Salicylic Acid on Growth, Yield, Qualityand Disease Pest Reaction of Onion (Allium Cepal.) CV. Agrifound Light Red

2020 ◽  
Vol 18 (1) ◽  
pp. 39-49
Author(s):  
P Bhasker ◽  
PK Gupta ◽  
HP Sharma
Keyword(s):  
Salicylic Acid ◽  
Plant Growth ◽  
Disease Incidence ◽  
Growth Yield ◽  
Harvest Management ◽  
Exogenous Application ◽  
Stemphylium Blight ◽  
Blight Disease ◽  
And Storage

Salicylic acid (SA) is endogenous naturally occurring plant growth hormone acting as an important signaling molecule adds tolerance against abiotic stress. A field experiment was conducted to assess the efficacy of exogenous application of SA on growth, yield and storage performances of onion during Rabi 2012-13, 2013-14 and 2014-15. The experiment was comprised of 6 different treatments of SA including control. Exogenous applications of all SA treatments significantly influenced plant growth and development. The treatment application of SA at 30 days after seed sowing and second spray at 30 days after transplanting and third spray at 60 days after transplanting performed superior in terms of growth, development and yield. Exogenous application of SA significantly influenced on thrips population and stemphylium blight disease incidence and intensity. The results also revealed that SA partially involved in post-harvest management of onion. SAARC J. Agri., 18(1):39-49 (2020)

scholarly journals Loose Plant Architecture 1-Interacting Kinesin-like Protein KLP Promotes Rice Resistance to Sheath Blight Disease

2021 ◽  
Author(s):  
Jin Chu ◽  
Han Xu ◽  
Hai Dong ◽  
Yuanhu Xuan
Keyword(s):  
Transcriptional Activation ◽  
Chromatin Immunoprecipitation ◽  
Plant Architecture ◽  
Wild Type ◽  
Yeast Two Hybrid ◽  
Sheath Blight Disease ◽  
Blight Disease ◽  
Two Hybrid ◽  
Wild Type Control

Abstract BackgroundSheath blight disease (ShB) is a destructive disease affecting rice production. Previously, we have reported that Loose Plant Architecture 1 (LPA1) promotes resistance to ShB. However, the mechanisms by which LPA1 confers resistance against this disease have not been extensively investigated. Notably, interactors that regulate LPA-1 activity remain elusive.FindingsIn this study, we identified the interaction of kinesin-like protein (KLP) with LPA1 in the nucleus of rice cells by yeast two-hybrid, bimolecular fluorescent complimentary (BiFC), and co-immunoprecipitation (co-IP) assays. To investigate the role of KLP in promoting resistance to ShB, wild-type, klp mutant, and KLP overexpressor (KLP OX) rice plants were inoculated with Rhizoctonia solani AG1-IA. The results indicated that, compared with the wild-type control, klp mutants were more susceptible while KLP OX plants were less susceptible to ShB. Since LPA1 transcriptionally activates PIN-FORMED 1a (PIN1a), we examined the expression of 8 related PIN genes. The results showed that only the expression of PIN1a and PIN3b coincided with KLP expression levels. In addition, a chromatin immunoprecipitation (ChIP) assay showed that KLP bound directly to the promoter region of PIN1a but not of PIN3b. Transient expression assays confirmed that LPA1 and KLP transcriptionally activate PIN1a, and that coexpression of KLP and LPA1 had an additive effect on the activation of PIN1a, suggesting that KLP enhances LPA1 transcriptional activation activity. ConclusionsTaken together, our results show that KLP is a novel LPA1 interactor that promotes resistance of rice to ShB.

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