scholarly journals Cloning, recombinant expression and characterization of a new glucoamylase gene from Aureobasidium pullulans NRRL 12974 and its potential application in raw potato starch degradation

2011 ◽  
Vol 10 (45) ◽  
pp. 9122-9131 ◽  
Author(s):  
Li Haifeng ◽  
Sun Wei ◽  
YunyunGao ◽  
YudanWu ◽  
Huang Lifeng ◽  
...  
Keyword(s):  
Potential Application ◽  
Aureobasidium Pullulans ◽  
Recombinant Expression ◽  
Potato Starch ◽  
Starch Degradation ◽  
Glucoamylase Gene

scholarly journals Degradation of Granular Starch by the Bacterium Microbacterium aurum Strain B8.A Involves a Modular α-Amylase Enzyme System with FNIII and CBM25 Domains

2015 ◽  
Vol 81 (19) ◽  
pp. 6610-6620 ◽  
Author(s):  
Vincent Valk ◽  
Wieger Eeuwema ◽  
Fean D. Sarian ◽  
Rachel M. van der Kaaij ◽  
Lubbert Dijkhuizen
Keyword(s):  
Enzyme System ◽  
Recombinant Expression ◽  
Potato Starch ◽  
Carbohydrate Binding ◽  
Starch Granules ◽  
Catalytic Core ◽  
Content Type ◽  
C Terminus ◽  
Carbohydrate Binding Modules

ABSTRACTThe bacteriumMicrobacterium aurumstrain B8.A, originally isolated from a potato plant wastewater facility, is able to degrade different types of starch granules. Here we report the characterization of an unusually large, multidomainM. aurumB8.A α-amylase enzyme (MaAmyA). MaAmyA is a 1,417-amino-acid (aa) protein with a predicted molecular mass of 148 kDa. Sequence analysis of MaAmyA showed that its catalytic core is a family GH13_32 α-amylase with the typical ABC domain structure, followed by a fibronectin (FNIII) domain, two carbohydrate binding modules (CBM25), and another three FNIII domains. Recombinant expression and purification yielded an enzyme with the ability to degrade wheat and potato starch granules by introducing pores. Characterization of various truncated mutants of MaAmyA revealed a direct relationship between the presence of CBM25 domains and the ability of MaAmyA to form pores in starch granules, while the FNIII domains most likely function as stable linkers. At the C terminus, MaAmyA carries a 300-aa domain which is uniquely associated with large multidomain amylases; its function remains to be elucidated. We concluded thatM. aurumB8.A employs a multidomain enzyme system to initiate degradation of starch granules via pore formation.

Synthesis and characterization of metal oxides complexes with potential application in HDS reactions

2021 ◽  
Vol 291 ◽  
pp. 129562
Author(s):  
S.L. Amaya ◽  
G. Alonso-Núñez ◽  
J.N. Díaz De León ◽  
S. Fuentes ◽  
Adriana Echavarría
Keyword(s):  
Metal Oxides ◽  
Potential Application ◽  
Synthesis And Characterization

The first sequenced Sphaerotilus natans bacteriophage– characterization and potential to control its filamentous bacterium host

2021 ◽  
Vol 97 (4) ◽  
Author(s):  
Rute Ferreira ◽  
Rui Amado ◽  
Jorge Padrão ◽  
Vânia Ferreira ◽  
Nicolina M Dias ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Bacterial Communities ◽  
Potential Application ◽  
Deleterious Effect ◽  
Wastewater Treatment Plants ◽  
Lytic Phage ◽  
Filamentous Bacterium ◽  
Sphaerotilus Natans ◽  
Isolation And Characterization

ABSTRACT Bacteriophages (phages) are ubiquitous entities present in every conceivable habitat as a result of their bacterial parasitism. Their prevalence and impact in the ecology of bacterial communities and their ability to control pathogens make their characterization essential, particularly of new phages, improving knowledge and potential application. The isolation and characterization of a new lytic phage against Sphaerotilus natans strain DSM 6575, named vB_SnaP-R1 (SnaR1), is here described. Besides being the first sequenced genome of a Sphaerotilus natans infecting phage, 99% of its 41507 bp genome lacks homology with any other sequenced phage, revealing its uniqueness and previous lack of knowledge. Moreover, SnaR1 is the first Podoviridae phage described infecting this bacterium. Sphaerotilus natans is an important filamentous bacterium due to its deleterious effect on wastewater treatment plants (WWTP) and thus, phages may play a role as novel biotechnological tools against filamentous overgrowth in WWTP. The lytic spectrum of SnaR1 was restricted to its host strain, infecting only one out of three S. natans strains and infection assays revealed its ability to reduce bacterial loads. Results suggest SnaR1 as the prototype of a new phage genus and demonstrates its potential as a non-chemical alternative to reduce S. natans DSM 6575 cells.

scholarly journals Recombinant Expression and Characterization of Human Activin A

1988 ◽  
Vol 2 (12) ◽  
pp. 1237-1242 ◽  
Author(s):  
Ralph H. Schwall ◽  
Karoly Nikolics ◽  
Eva Szonyi ◽  
Cori Gorman ◽  
Anthony J. Mason
Keyword(s):  
Recombinant Expression ◽  
Activin A

Partial characterization of sulfohydrolase from Gracilaria dura and evaluation of its potential application in improvement of the agar quality

2011 ◽  
Vol 85 (1) ◽  
pp. 157-163 ◽  
Author(s):  
Mahendra K. Shukla ◽  
Manoj Kumar ◽  
Kamlesh Prasad ◽  
C.R.K. Reddy ◽  
Bhavanath Jha
Keyword(s):  
Potential Application ◽  
Partial Characterization ◽  
Agar Quality ◽  
Gracilaria Dura
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