Isolation and characterization of Gram negative obligate and facultative alkalophilic Bacillus sp. from desert soil of Saudi Arabia

2012 ◽  
Vol 11 (41) ◽  
Author(s):  
Abdulrahman S. A. Assaeedi
Keyword(s):  
Saudi Arabia ◽  
Desert Soil ◽  
Bacillus Sp ◽  
Gram Negative ◽  
Isolation And Characterization ◽  
Alkalophilic Bacillus

scholarly journals Isolation and characterization of halophilic Bacillus sp. BS3 able to produce pharmacologically important biosurfactants

2013 ◽  
Vol 6 (11) ◽  
pp. 876-883 ◽  
Author(s):  
MBS Donio ◽  
SFA Ronica ◽  
V Thanga Viji ◽  
S Velmurugan ◽  
J Adlin Jenifer ◽  
...  
Keyword(s):  
Bacillus Sp ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Pb Resistant Bacteria from Cilalay Lake, Indonesia

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Kesi Kurnia ◽  
Nina Hermayani Sadi ◽  
Syafitri Jumianto
Keyword(s):  
Heterotrophic Bacteria ◽  
Water Environment ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Gram Negative ◽  
Isolation And Characterization ◽  
Agar Media ◽  
Physical And Chemical ◽  
Standard Disk

<span>Pollution of water environment with heavy metals is becoming one of the most severe environmental and human health hazards. Lead (Pb) is a major pollutant and highly toxic to human, animals, plants, and microbes. </span><span lang="IN">Toxic metals are difficult to remove from the environment, since they cannot be chemically or biologically degraded and are ultimately indestructible. Biological approaches based on metal-resistant microorganisms have received a great deal of attention as alternative remediation processes. </span><span>This study aim to isolat</span><span lang="IN">e</span><span> and characterize Pb resistant of heterotrophic bacteria in Cilalay Lake, </span><span lang="IN">West Java, </span><span>Indonesia. The water samples were collected </span><span lang="IN">along</span><span> three points around Cilalay Lake. </span><span lang="IN">Water physical and chemical </span><span>determination was performed using the Water Quality Checker</span><span lang="IN">. </span><span>The bacterial isolates were screened on T</span><span lang="IN">r</span><span>ipton</span><span lang="IN">e</span><span> Glucose Yeast (TGY) agar plates. </span><span lang="IN">Afterwards s</span><span>elected isolates were grown on Nutrient Agar media 50% </span><span lang="IN">with </span><span>supplemented Pb 100 ppm by the standard disk. Population of resistant bacteria was counted. The result from metal resistant bacteria indicated that all isolates w</span><span lang="IN">ere</span><span> resistant. The most abundant type of resistant </span><span lang="IN">bacteria </span><span>to lead was Gram negative more than Gram positive. Identified have metal resistant bacteria could be useful for the bioremediation of heavy metal contaminated sewage and waste water</span>

THE ISOLATION AND CHARACTERIZATION OF METHANOMONAS METHANOOXIDANS BROWN AND STRAWINSKI

1964 ◽  
Vol 10 (5) ◽  
pp. 791-799 ◽  
Author(s):  
L. R. Brown ◽  
R. J. Strawinski ◽  
C. S. McCleskey
Keyword(s):  
Formic Acid ◽  
Inorganic Nitrogen ◽  
Nitrogen Sources ◽  
Energy Sources ◽  
Resting Cells ◽  
Gram Negative ◽  
Oxidation Of Methane ◽  
Isolation And Characterization ◽  
Trapping Agent

Procedures for the isolation and characterization of Metkanomonas methanooxidans Brown and Strawinski are described. Isolates from varied sources are alike in cellular morphology, inasmuch as they form only microcolonies, and in their dependence on methane or methanol as carbon and energy sources for growth. Both organic and inorganic nitrogen sources are used. The organism is a Gram negative non-sporeforming rod, 1.5 to 3.0 μ by 1.0 μ in size, and motile by means of a single polar flagellum. In growing cultures the oxygen/methane ratio was approximately 1.1 and in resting cells 1.7. The R.Q. for methane with resting cells was 0.43. Resting cells were unable to oxidize organic compounds other than methane, methanol, formaldehyde, and formate. Formic acid was detected in test solutions after cell suspensions had metabolized methane, methanol, and formaldehyde. Using sodium sulphite as trapping agent for formaldehyde, it was found that 60 to 70% of the methane or methanol consumed was converted to formaldehyde. In the presence of iodoacetate, 70% of the methane consumed was present terminally as methanol. Thus it was shown that methanol, formaldehyde, and formic acid are sequential intermediates in the oxidation of methane by these organisms.

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