Localization of immunodominant linear B-cell epitopes of Vibrio mimicus outer membrane protein U (OmpU)

2012 ◽  
Vol 11 (35) ◽  
Author(s):  
Ping-Ping Wang
Keyword(s):  
Membrane Protein ◽  
B Cell ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
B Cell Epitopes ◽  
Vibrio Mimicus ◽  
Linear B

Use of synthetic peptides to identify surface-exposed, linear B-cell epitopes within outer membrane protein F of Pseudomonas aeruginosa

1995 ◽  
Vol 31 (5) ◽  
pp. 279-286 ◽  
Author(s):  
Harry E. Gilleland ◽  
Eileen E. Hughes ◽  
Linda B. Gilleland ◽  
Janice M. Matthews-Greer ◽  
John Staczek
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Membrane Protein ◽  
B Cell ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Synthetic Peptides ◽  
B Cell Epitopes ◽  
Protein F ◽  
Outer Membrane Protein F ◽  
Linear B

scholarly journals Identification of Immunogenic Linear B-Cell Epitopes in C. burnetii Outer Membrane Proteins Using Immunoinformatics Approaches Reveals Potential Targets of Persistent Infections

Pathogens ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1250
Author(s):  
Sílvia da Silva Fontes ◽  
Fernanda de Moraes Maia ◽  
Laura Santa’Anna Ataides ◽  
Fernando Paiva Conte ◽  
Josué da Costa Lima-Junior ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
B Cell ◽  
Outer Membrane ◽  
Q Fever ◽  
Outer Membrane Proteins ◽  
Humoral Response ◽  
Neglected Diseases ◽  
B Cell Epitopes ◽  
Wide Range ◽  
Linear B

Coxiella burnetii is a global, highly infectious intracellular bacterium, able to infect a wide range of hosts and to persist for months in the environment. It is the etiological agent of Q fever—a zoonosis of global priority. Currently, there are no national surveillance data on C. burnetii’s seroprevalence for any South American country, reinforcing the necessity of developing novel and inexpensive serological tools to monitor the prevalence of infections among humans and animals—especially cattle, goats, and sheep. In this study, we used immunoinformatics and computational biology tools to predict specific linear B-cell epitopes in three C. burnetii outer membrane proteins: OMP-H (CBU_0612), Com-1 (CBU_1910), and OMP-P1 (CBU_0311). Furthermore, predicted epitopes were tested by ELISA, as synthetic peptides, against samples of patients reactive to C. burnetii in indirect immunofluorescence assay, in order to evaluate their natural immunogenicity. In this way, two linear B-cell epitopes were identified in each studied protein (OMP-H(51–59), OMP-H(91–106), Com-1(57–76), Com-1(191–206), OMP-P1(197–209), and OMP-P1(215–227)); all of them were confirmed as naturally immunogenic by the presence of specific antibodies in 77% of studied patients against at least one of the identified epitopes. Remarkably, a higher frequency of endocarditis cases was observed among patients who presented an intense humoral response to OMP-H and Com-1 epitopes. These data confirm that immunoinformatics applied to the identification of specific B-cell epitopes can be an effective strategy to improve and accelerate the development of surveillance tools against neglected diseases.

scholarly journals Evolution of Salmonella Typhi outer membrane protein-specific T and B cell responses in humans following oral Ty21a vaccination: A randomized clinical trial

PLoS ONE ◽  
2017 ◽  
Vol 12 (6) ◽  
pp. e0178669 ◽  
Author(s):  
Juan Manuel Carreño ◽  
Christian Perez-Shibayama ◽  
Cristina Gil-Cruz ◽  
Constantino Lopez-Macias ◽  
Pietro Vernazza ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Membrane Protein ◽  
B Cell ◽  
Outer Membrane ◽  
Randomized Clinical Trial ◽  
Outer Membrane Protein ◽  
Salmonella Typhi ◽  
Cell Responses ◽  
B Cell Responses
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