A genetic linkage map of Japanese scallop Mizuhopecten yessoensis based on amplified fragment length polymorphism (AFLP) and microsatellite (SSR) markers

2012 ◽  
Vol 11 (46) ◽  
Author(s):  
Meng Chen,
Keyword(s):  
Linkage Map ◽  
Ssr Markers ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Japanese Scallop ◽  
Mizuhopecten Yessoensis

scholarly journals Construction of a Genetic Linkage Map Based on Amplified Fragment Length Polymorphism Markers and Development of Sequence-Tagged Site Markers for Marker-Assisted Selection of the Sporeless Trait in the Oyster Mushroom (Pleurotus eryngii)

2011 ◽  
Vol 78 (5) ◽  
pp. 1496-1504 ◽  
Author(s):  
Yasuhito Okuda ◽  
Jun Ueda ◽  
Yasushi Obatake ◽  
Shigeyuki Murakami ◽  
Yukitaka Fukumasa ◽  
...  
Keyword(s):  
Linkage Map ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Marker Assisted Selection ◽  
Genetic Linkage Map ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Content Type ◽  
Sequence Tagged Site ◽  
Site Markers

ABSTRACTA large number of spores from fruiting bodies can lead to allergic reactions and other problems during the cultivation of edible mushrooms, includingPleurotus eryngii(DC.) Quél. A cultivar harboring a sporulation-deficient (sporeless) mutation would be useful for preventing these problems, but traditional breeding requires extensive time and labor. In this study, using a sporelessP. eryngiistrain, we constructed a genetic linkage map to introduce a molecular breeding program like marker-assisted selection. Based on the segregation of 294 amplified fragment length polymorphism markers, two mating type factors, and the sporeless trait, the linkage map consisted of 11 linkage groups with a total length of 837.2 centimorgans (cM). The gene region responsible for the sporeless trait was located in linkage group IX with 32 amplified fragment length polymorphism markers and theBmating type factor. We also identified eight markers closely linked (within 1.2 cM) to the sporeless locus using bulked-segregant analysis-based amplified fragment length polymorphism. One such amplified fragment length polymorphism marker was converted into two sequence-tagged site markers, SD488-I and SD488-II. Using 14 wild isolates, sequence-tagged site analysis indicated the potential usefulness of the combination of two sequence-tagged site markers in cross-breeding of the sporeless strain. It also suggested that a map constructed forP. eryngiihas adequate accuracy for marker-assisted selection.

The construction of a preliminary genetic linkage map in the Japanese scallop Mizuhopecten yessoensis

2009 ◽  
Vol 31 (6) ◽  
pp. 629-637 ◽  
Author(s):  
Wei-Dong LIU ◽  
Xiang-Bo BAO ◽  
Wen-Tao SONG ◽  
Zun-Chun ZHOU ◽  
Chong-Bo HE ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Japanese Scallop ◽  
Mizuhopecten Yessoensis

scholarly journals A Genetic Linkage Map of Olive Based on Amplified Fragment Length Polymorphism, Intersimple Sequence Repeat and Simple Sequence Repeat Markers

2010 ◽  
Vol 135 (6) ◽  
pp. 548-555 ◽  
Author(s):  
Bouchaib Khadari ◽  
Amal Zine El Aabidine ◽  
Cinderella Grout ◽  
Inès Ben Sadok ◽  
Agnès Doligez ◽  
...  
Keyword(s):  
Linkage Map ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Simple Sequence Repeat ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Issr Markers ◽  
Phenotypic Characterization ◽  
Sequence Repeat ◽  
Simple Sequence

A detailed genomic linkage map of the olive [Olea europaea L. ssp. europaea (2x = 2n = 46)] was constructed with a 147 F1 full-sib ‘Olivière’ × ‘Arbequina’ progeny in a two-way pseudo-test cross-mapping configuration. Based on a logarithm of odds threshold of 6 and a maximum recombination fraction of 0.4, maternal and paternal maps were constructed using 222 makers [178 amplified fragment length polymorphism (AFLP), 37 simple sequence repeat (SSR), seven intersimple sequence repeat (ISSR)] and 219 markers (174 AFLP, 39 SSR, 6 ISSR) markers, respectively. The female map regrouped 36 linkage groups (LGs) defining 2210.2 cM of total map length with an average marker spacing 11.2 cM and a maximum gap of 48.5 cM between adjacent markers. The male map contained 31 LGs and covered a distance of 1966.2 cM with an average and a maximum distance between two adjacent markers of 10.3 and 40.4 cM, respectively. Mean LG size was 61.3 and 63.4 cM in the maternal and paternal maps, respectively. The LGs consisted of two to 17 loci (up to 21 loci in the paternal map) and ranged in length from 2.7 to 182 cM (female map) or from 4.1 to 218.1 cM (paternal map). Markers were distributed throughout the maps without any clustering. The total length of the consensus map was 3823.2 cM containing 436 markers distributed into 42 LGs with a mean distance between two adjacent loci of 8.7 cM. Both parental maps and the consensus maps were compared with previously published olive maps. Although not saturated yet, the present maps offer a promising tool for quantitative trait loci mapping because phenotypic characterization of the cross is currently carried out.

The molecular–genetic analysis of Triticum tauschii, the D-genome donor to hexaploid wheat

Genome ◽  
1991 ◽  
Vol 34 (3) ◽  
pp. 375-386 ◽  
Author(s):  
E. S. Lagudah ◽  
R. Appels ◽  
A. H. D. Brown ◽  
D. McNeil
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Fragment Length Polymorphism ◽  
Repetitive Sequences ◽  
D Genome ◽  
Genome Donor ◽  
Restriction Fragment Length

DNA from Triticum tauschii (the D-genome donor to hexaploid wheat, Triticum aestivum) has been cloned using the restriction endonuclease PstI to generate fragments for insertion into the plasmid pBR322 or pUC118. A total of 143 clones were studied and demonstrated to contain one of the following sequence types: (i) a D-genome amplified repetitive sequence, (ii) polymorphic repetitive sequences ("fingerprint-type" sequences), (iii) polymorphic low to moderately repetitive sequences (PLR sequences), (iv) polymorphic low copy number sequences (PL sequences), and (v) invariant sequences. The D-genome amplified sequence was found to be located on all seven chromosomes. A genetic linkage map using PL and PLR sequences was produced by analysing F2 segregating progeny from crosses between two different taxa of T. tauschii. In addition to using DNA clones from T. tauschii, clones from other laboratories containing either anonymous sequences or genes coding for known products (e.g., 7S globulin, dehydrin, germin, storage protein) were used in the genetic linkage map. Multiple locations were mapped for the PLR sequences and were often clustered on single chromosomes. The restriction fragment length polymorphism markers and isozymes analysed were generally distributed over all the linkage groups that were identified and, when used in conjunction with published markers, provided the basis for a genetic map of T. tauschii.Key words: D genome, genetic linkage, restriction fragment length polymorphism, isozymes, chromosomal location.

An Amplified Fragment Length Polymorphism Map of the Silkworm

Genetics ◽  
2001 ◽  
Vol 157 (3) ◽  
pp. 1277-1284 ◽  
Author(s):  
Yuan-De Tan ◽  
Chunling Wan ◽  
Yufang Zhu ◽  
Chen Lu ◽  
Zhonghuai Xiang ◽  
...  
Keyword(s):  
Linkage Group ◽  
Linkage Map ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Aflp Markers ◽  
Polymorphic Markers ◽  
Linkage Groups ◽  
Group 3

Abstract The silkworm (Bombyx mori L.) is a lepidopteran insect with a long history of significant agricultural value. We have constructed the first amplified fragment length polymorphism (AFLP) genetic linkage map of the silkworm B. mori at a LOD score of 2.5. The mapping AFLP markers were genotyped in 47 progeny from a backcross population of the cross no. 782 × od100. A total of 1248 (60.7%) polymorphic AFLP markers were detected with 35 PstI/TaqI primer combinations. Each of the primer combinations generated an average of 35.7 polymorphic AFLP markers. A total of 545 (44%) polymorphic markers are consistent with the expected segregation ratio of 1:1 at the significance level of P = 0.05. Of the 545 polymorphic markers, 356 were assigned to 30 linkage groups. The number of markers on linkage groups ranged from 4 to 36. There were 21 major linkage groups with 7-36 markers and 9 relatively small linkage groups with 4-6 markers. The 30 linkage groups varied in length from 37.4 to 691.0 cM. The total length of this AFLP linkage map was 6512 cM. Genetic distances between two neighboring markers on the same linkage group ranged from 0.2 to 47 cM with an average of 18.2 cM. The sex-linked gene od was located between the markers P1T3B40 and P3T3B27 at the end of group 3, indicating that AFLP linkage group 3 was the Z (sex) chromosome. This work provides an essential basic map for constructing a denser linkage map and for mapping genes underlying agronomically important traits in the silkworm B. mori L.

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