Effective extraction of cephalosporin C from whole fermentation broth of Acremonium chrysogenum utilizing aqueous two phase systems

2012 ◽  
Vol 11 (31) ◽  
Author(s):  
Amina Wajid
Keyword(s):  
Fermentation Broth ◽  
Cephalosporin C ◽  
Acremonium Chrysogenum ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Partitioning behavior of recombinant lipase in Escherichia coli by ionic liquid-based aqueous two-phase systems

RSC Advances ◽  
2016 ◽  
Vol 6 (86) ◽  
pp. 82571-82580 ◽  
Author(s):  
Muhammad Hakimi Hadzir ◽  
Sahar Abbasiliasi ◽  
Arbakariya B. Ariff ◽  
Siti Baidurah Yusoff ◽  
Hui Suan Ng ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ionic Liquid ◽  
Ionic Liquids ◽  
Fermentation Broth ◽  
Two Phase ◽  
E Coli ◽  
Recombinant Lipase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Partitioning Behavior

Evaluations of ILATPSs were performed with a variety of ionic liquids and salts as phase components to figure out their competencies in the recovery of lipase from a fermentation broth of E. coli using banana waste as a substrate.

scholarly journals Primary recovery of hyaluronic acid produced in Streptococcus equi subsp. zooepidemicus using PEG–citrate aqueous two-phase systems

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Miguel Flores-Gatica ◽  
Héctor Castañeda-Aponte ◽  
Mónica Rebeca Gil-Garzon ◽  
Liliana Monserrath Mora-Galvez ◽  
Martin Paul Banda-Magaña ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Negative Impact ◽  
Fermentation Broth ◽  
Downstream Processing ◽  
Bottom Phase ◽  
Two Phase ◽  
Streptococcus Equi ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
First Time

AbstractGiven its biocompatibility, rheological, and physiological properties, hyaluronic acid (HA) has become a biomaterial of increasing interest with multiple applications in medicine and cosmetics. In recent decades, microbial fermentations have become an important source for the industrial production of HA. However, due to its final applications, microbial HA must undergo critical and long purification processes to ensure clinical and cosmetic grade purity. Aqueous two-phase systems (ATPS) have proven to be an efficient technique for the primary recovery of high-value biomolecules. Nevertheless, their implementation in HA downstream processing has been practically unexplored. In this work, polyethylene glycol (PEG)–citrate ATPS were used for the first time for the primary recovery of HA produced with an engineered strain of Streptococcus equi subsp. zooepidemicus. The effects of PEG molecular weight (MW), tie-line length (TLL), volume ratio (VR), and sample load on HA recovery and purity were studied with a clarified fermentation broth as feed material. HA was recovered in the salt-rich bottom phase, and its recovery increased when a PEG MW of 8000 g mol−1 was used. Lower VR values (0.38) favoured HA recovery, whereas purity was enhanced by a high VR (3.50). Meanwhile, sample load had a negative impact on both recovery and purity. The ATPS with the best performance was PEG 8000 g mol−1, TLL 43% (w/w), and VR 3.50, showing 79.4% HA recovery and 74.5% purity. This study demonstrated for the first time the potential of PEG–citrate ATPS as an effective primary recovery strategy for the downstream process of microbial HA.

Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems

2009 ◽  
Vol 14 (6) ◽  
pp. 811-818 ◽  
Author(s):  
Chien Wei Ooi ◽  
Beng Ti Tey ◽  
Siew Ling Hii ◽  
Arbakariya Ariff ◽  
Ho Shing Wu ◽  
...  
Keyword(s):  
Burkholderia Pseudomallei ◽  
Fermentation Broth ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

scholarly journals Bacteriocin partitioning from a clarified fermentation broth of Lactobacillus plantarum ST16Pa in aqueous two-phase systems with sodium sulfate and choline-based salts as additives

2018 ◽  
Vol 66 ◽  
pp. 212-221 ◽  
Author(s):  
Sabrina da Silva Sabo ◽  
André Moreni Lopes ◽  
Valéria de Carvalho Santos-Ebinuma ◽  
Carlota de Oliveira Rangel-Yagui ◽  
Ricardo Pinheiro de Souza Oliveira
Keyword(s):  
Lactobacillus Plantarum ◽  
Sodium Sulfate ◽  
Fermentation Broth ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Extraction of tetracycline from fermentation broth using aqueous two-phase systems composed of polyethylene glycol and cholinium-based salts

2013 ◽  
Vol 48 (4) ◽  
pp. 716-722 ◽  
Author(s):  
Jorge F.B. Pereira ◽  
Filipa Vicente ◽  
Valéria C. Santos-Ebinuma ◽  
Janete M. Araújo ◽  
Adalberto Pessoa ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Fermentation Broth ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Continuous purification of antibodies from cell culture supernatant with aqueous two-phase systems: From concept to process

2013 ◽  
Vol 8 (3) ◽  
pp. 352-362 ◽  
Author(s):  
Paula A. J. Rosa ◽  
Ana M. Azevedo ◽  
S. Sommerfeld ◽  
Martina Mutter ◽  
Werner Bäcker ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Supernatant ◽  
Cell Culture Supernatant ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Optimized and Scalable Green Extraction of Pristimerin, an Anticancerigen from Mortonia greggii, by Ethanol–Phosphate Aqueous Two-Phase Systems

2021 ◽  
Vol 60 (15) ◽  
pp. 5403-5410
Author(s):  
Martha Alicia González-Félix ◽  
Luis Alberto Mejía-Manzano ◽  
Bertha A. Barba-Dávila ◽  
Sergio O. Serna-Saldívar ◽  
José González-Valdez
Keyword(s):  
Two Phase ◽  
Green Extraction ◽  
Aqueous Two Phase Systems ◽  
Phase Systems
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