High frequency shoot organogenesis in juvenile leaf of Duchesnea indica (Andr.) Focke

2012 ◽  
Vol 11 (17) ◽  
Author(s):  
Jiang Xiang-Hui
Keyword(s):  
High Frequency ◽  
Shoot Organogenesis ◽  
Juvenile Leaf ◽  
Duchesnea Indica

High frequency plant regeneration following abnormal shoot organogenesis in the medicinal tree Hovenia dulcis

2009 ◽  
Vol 98 (1) ◽  
pp. 59-65 ◽  
Author(s):  
Mi Jin Jeong ◽  
Hyun Jin Song ◽  
Dong Jin Park ◽  
Ji Yun Min ◽  
Jin Seong Jo ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
High Frequency ◽  
Shoot Organogenesis ◽  
Medicinal Tree ◽  
Hovenia Dulcis

scholarly journals A TISSUE CULTURE APPROACH FOR DEVELOPING TETRAPLOID WATERMELONS

HortScience ◽  
1996 ◽  
Vol 31 (5) ◽  
pp. 747b-747
Author(s):  
Xingping Zhang ◽  
Billy B. Rhodes
Keyword(s):  
Tissue Culture ◽  
High Frequency ◽  
Shoot Organogenesis ◽  
Leaf Shape ◽  
Low Frequency ◽  
Female Fertility ◽  
Leaf Margin ◽  
The Past ◽  
Colchicine Solution ◽  
Seed Characteristics

Tetraploids are needed to synthesize triploid watermelons, which produce “seedless” fruit with improved quality. Traditionally, the tetraploids are induced by applying colchicine to the growing apex of seedlings or soaking the seeds with colchicine solution. This method often produces low frequency of tetraploids and high frequency of chimeras. Breeding tetraploids takes much longer time than breeding diploids because of the low female fertility. We developed a tissue culture approach that allows breeders to develop desirable tetraploids with commercially acceptable volume of seed in 2 years. This tissue culture approach includes: 1) regenerate plants via shoot organogenesis from cotyledon tissue; 2) screen tetraploids based on leaf morphology (more serrated leaf margin and wider leaf shape) before transplanting, and confirm tetraploids based on pollen morphology (larger pollen with four copi) and/or seed characteristics; 3) self-pollinate tetraploids or cross the tetraploids with diploids to accurately estimate the female fertility; 4) micropropagate the best tetraploid(s) using axillary buds during the off-season; and 5) produce tetraploid seed from the cloned tetraploids in an isolation plot and evaluate the triploids derived from the tetraploid(s) in the following season. This approach has been practiced on more than 20 genotypes over the past 4 years.

High-Frequency Shoot Regeneration From Flower Bud Derived Callus of Gymnostachyum Febrifugum Benth., an Endemic Medicinal Plant to the Western Ghats

2021 ◽  
Author(s):  
Silpa P ◽  
Dennis Thuruthiyil Thomas
Keyword(s):  
Callus Induction ◽  
High Frequency ◽  
Shoot Organogenesis ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Flower Bud ◽  
The Family ◽  
Endemic Medicinal Plant ◽  
Dichlorophenoxy Acetic Acid ◽  
The Western Ghats

Abstract Gymnostachyum febrifugum Benth. is a small, scapigerous, rare and endemic medicinal herb indigenous to India belonging to the family Acanthaceae. This study reports an efficient protocol for high-frequency flower bud derived callus induction and shoot organogenesis in G. febrifugum. Flower buds at 7d before anthesis (dBA) were excised from the inflorescence and cultured on MS medium supplemented with various concentrations of 2, 4-dichlorophenoxy acetic acid (2, 4-D; 0.5-2.0 mg/l) for callus induction. The optimum callus induction (78%) was obtained on MS medium supplemented with 1.5 mg/l 2, 4-D. The calli when subcultured on MS medium supplemented with different concentrations of thidiazuron (TDZ; 0.5-2.5 mg/l) or 6-benzylaminopurine BAP (0.5-2.5 mg/l) alone or in combination with 1- naphthaleneacetic acid (NAA; 0.2-0.7 mg/l) induced shoots. The highest frequency (94%) and number of shoots (44.6 shoots/unit callus) were obtained on MS medium supplemented with 2.0 mg/l TDZ and 0.5 mg/l NAA. The optimum rooting frequency (95%) and number of roots (10.2) were observed on ½ MS medium supplemented with 3.0 mg/l indole-3- butyric acid (IBA). The rooted plantlets were acclimatized and transferred to soil with 94% success.

Thidiazuron induces high-frequency indirect shoot organogenesis of Bienertia sinuspersici: a single-cell C4 species

2016 ◽  
Vol 126 (1) ◽  
pp. 141-151 ◽  
Author(s):  
Jennifer Anne Northmore ◽  
Dustin Sigurdson ◽  
Sarah Schoor ◽  
Amer Rustum ◽  
Simon D. X. Chuong
Keyword(s):  
Single Cell ◽  
High Frequency ◽  
Shoot Organogenesis ◽  
Bienertia Sinuspersici ◽  
C4 Species

scholarly journals THIDIAZURON: A POTENT GROWTH REGULATOR FOR INDUCING HIGH-FREQUENCY ORGANOGENESIS AND SOMATIC EMBRYOGENESIS IN VITRO

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 693a-693
Author(s):  
K.A. Malik ◽  
Christena Visser ◽  
praveen K. saxena
Keyword(s):  
Somatic Embryogenesis ◽  
High Frequency ◽  
Somatic Embryos ◽  
Direct Evidence ◽  
Shoot Organogenesis ◽  
In Vitro Regeneration ◽  
Endogenous Phytohormones ◽  
Potent Growth

In vitro regeneration by shoot organogenesis and-or somatic embryogenesis is accomplished by culturing the explants on a nutrient medium supplemented with phytohormones. Auxins in general, and 2,4-D in particular, have been shown to induce somatic embryogenesis whereas shoot regeneration is stimulated by cytokinins. In studying the morphoregulatory role of thidiazuron (TDZ) - a substituted urea with cytokinin-like activity - we found that it induces a high frequency of both organogenesis and somatic embryogenesis depending upon the plant species. For instance, whole seedlings of peanut developed somatic embryos and those of bean and pea produced shoots in response to culture on TDZ (1-40 μM)-supplemented media. In cultured explants of geranium, the use of TDZ (0.2-1 μM) effectively replaced the requirement of 2,4-D or BAP and IAA for obtaining somatic embryos. The frequency of regeneration was two to ten times higher than that achieved with auxin-cytokinin combinations. While no direct evidence is currently available to establish a relationship between TDZ and endogenous phytohormones, our results suggest that it may act by establishing endogenously the auxin:cytokinin ratio permissive of induction and expression of morphogenically competent cells.

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