Morphogenesis of in vitro main root transverse thin cell layers of Vietnamese ginseng (Panax vietnamensis Ha et Grushv.)

2012 ◽  
Vol 11 (23) ◽  
Author(s):  
Nhut, D. T
Keyword(s):  
Main Root ◽  
Thin Cell Layers ◽  
Cell Layers ◽  
Transverse Thin Cell Layers

Morphological and physiological characteristics of rapeseed plants regenerated in vitro from thin cell layers in the presence of zinc

2007 ◽  
Vol 330 (10) ◽  
pp. 728-734 ◽  
Author(s):  
Asma Ben Ghnaya ◽  
Gilbert Charles ◽  
Annick Hourmant ◽  
Jeannette Ben Hamida ◽  
Michel Branchard
Keyword(s):  
Physiological Characteristics ◽  
Thin Cell Layers ◽  
Cell Layers

scholarly journals Primary and secondary somatic embryogenesis in Jatropha curcas L. From leaf transverse thin cell layers

2018 ◽  
Vol 14 (4) ◽  
pp. 661-671
Author(s):  
Nguyen Thi Kim Loan ◽  
Do Dang Giap ◽  
Tran Trong Tuan ◽  
Nguyen Thi Thanh Hien ◽  
Nguyen Phuc Huy ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Jatropha Curcas ◽  
Somatic Embryos ◽  
Formation Rate ◽  
Ms Medium ◽  
Secondary Somatic Embryogenesis ◽  
Thin Cell Layers ◽  
Cell Layers ◽  
Secondary Embryos ◽  
Transverse Thin Cell Layers

An efficient method for plant regeneration in Jatropha curcas L. via primary and secondary somatic embryogenesis culture from ex vitro leaves of 6-month-old plants was presented in this study. Leaves were cut into transverse thin cell layers (tTCLs) and cultured on MS medium supplemented with kinetin (KIN) at 0.5, 1.0, 1.5, and 2.0 mg/l in combination with indole-3-butyric acid (IBA) at 0.1, 0.5, and 1.0 mg/l or 2,4-dichlorophenoxyacetic acid (2,4-D) at 1.0, 1.5 and 2.0 mg/l . The highest embryogenic callus formation rate (89.3%) was obtained on medium supplemented with 1.0 mg/l KIN and 1.5 mg/l 2,4-D. The calli were selected for the study of primary somatic embryogenesis on MS medium containing 2,4-D (0.01, 0.03, 0.05, and 0.07 mg/l) or KIN (0.5, 1.0, 1.5, and 2.0 mg/l). The highest primary somatic embryos formation rate (76.67%) was achieved on MS medium supplemented with 1.0 mg/l KIN. The primary embryos were cultured on medium supplemented with KIN (0.1, 0.5, 1.0, 1.5, and 2.0 mg/l) combined with 0.2 mg/l indole-3-butyric acid (IBA) or 0.05 mg/l 2,4-D. The combination of 1.5 mg/l KIN and 0.05 mg/l 2,4-D was suitable for secondary embryos formation. Embryos proliferated rapidly, and the highest number of secondary embryos (77.5 embryos) wasobtained from a single primary embryos inoculated. Results also showed that the addition of proline (0.75 g/l) or spermidine (0.15 mM) to the culture medium increased the number of secondary embryos considerably. The fully developed plantlets exhibiting healthy roots and shoots were obtained when somatic embryos were sub-cultured onto B5 medium containing 1.5 mg/l IBA.

scholarly journals High frequency plant regeneration from transverse thin cell layers in Indian mustard (Brassica juncea L.)

2009 ◽  
Vol 36 (1) ◽  
pp. 81-86 ◽  
Author(s):  
Mohammed Shafi Ullah Bhuiyan ◽  
Yong-Pyo Lim ◽  
Sung-Ran Min ◽  
Kwan-Sam Choi ◽  
Jang-R. Liu
Keyword(s):  
Plant Regeneration ◽  
Brassica Juncea ◽  
High Frequency ◽  
Indian Mustard ◽  
Thin Cell Layers ◽  
Cell Layers ◽  
Transverse Thin Cell Layers

scholarly journals POTENCIAL DE REGENERACIÓN DE Psychotria ipecacuanha (Rubiaceae) A PARTIR DE CAPAS DELGADAS DE CÉLULAS

2015 ◽  
Vol 20 (3) ◽  
pp. 181-192 ◽  
Author(s):  
Catalina BOTERO GIRALDO ◽  
Aura Inés RREA TRUJILLO ◽  
Esther Julia NARANJO GÓMEZ
Keyword(s):  
Growth Regulators ◽  
Somatic Embryos ◽  
Light Condition ◽  
Nodal Explants ◽  
Light Emitting ◽  
Thin Cell Layers ◽  
Cell Layers ◽  
Medicinal Properties ◽  
Leaf Segments

<p>Conociendo las propiedades medicinales de la especie vegetal <em>Psychotria</em> <em>ipecacuanha</em> (Brot.) Stokes, su crítico estado de conservación, así como las dificultades que presenta para la propagación efectiva, el presente estudio tuvo como objetivo evaluar su potencial de propagación por los sistemas de regeneración <em>in vitro</em>, organogénesis y embriogénesis somática. Para este propósito, capas delgadas de células (CDCs) de tallos y de hojas, así como segmentos foliares fueron sometidos a diferentes tratamientos con reguladores de crecimiento y condiciones de luz. Además se estableció el efecto de diferentes longitudes de onda vía diodos emisores de luz (LEDs), sobre la regeneración en estos explantes y nudos provenientes de plantas <em>in vitro</em>. Los resultados obtenidos mostraron que los segmentos de hoja y las CDCs de tallo sembrados en el medio de cultivo MS suplementado con las combinaciones de los reguladores de crecimiento IBA + BAP e IBA + TDZ formaron embriones somáticos y brotes. Los cortes histológicos realizados corroboraron estos dos tipos de origen. Se encontró que bajo la condición lumínica 16/8, se alcanzaron los mejores resultados de inducción de brotes y embriones. En cuanto al efecto de las diferentes longitudes de onda de luz, se encontró que las correspondientes al rojo, verde y blanca, favorecieron el crecimiento y desarrollo de brotes y la inducción de embriones somáticos. El desarrollo de los brotes a partir de los nudos no presentó diferencias estadísticas entre los tratamientos con LEDs, por lo que se recomienda el uso de la luz blanca continua y con fotoperiodo durante el proceso de multiplicación y desarrollo de estos.</p><p><strong>Regeneration Potential of <em>Psychotria Ipecacuanha</em> (Rubiaceae) from Thin Cell Layers </strong></p><p>Knowing the medicinal properties of the plant specie <em>Psychotria ipecacuanha</em> (Brot.) Stokes, its critical condition and the difficulties for its effective propagation, the present study aimed to assess the potential of propagation of <em>P. ipecacuanha</em> by <em>in vitro</em> systems of regeneration, organogenesis and somatic embryogenesis. For this purpose, thin cell layers (TCL) of stems and leaves, as well as leaf segments were subjected to different treatments of plant growth regulators and light conditions. Furthermore, the effect of different wavelengths via light emitting diodes (LED’s), was established for the regeneration in these explants and nodal explants from <em>in vitro</em> plants. The results showed that leaf segments and stem TCL, cultured in MS medium supplemented with the combinations of growth regulators IBA + BAP and IBA + TDZ, formed somatic embryos and shoots. The histological sections supported the two types of source. It was found that the best results in shoot induction and embryos were achieved under the light condition 16/8-h light/dark. Regarding the effect of different wavelengths, it was found that those corresponding to red, green, and white supported the growth and shoot development as well as somatic embryos induction. The shoots development from the nodal explants did not show statistical differences between LEDs treatments, so the use of a continuous white light and photoperiod is recommended during their multiplication and development.</p>

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