GFLV coat protein constructs based on local isolates from the Czech Republic

2012 ◽  
Vol 11 (5) ◽  
Author(s):  
Josef Vlasák
Keyword(s):  
Czech Republic ◽  
Coat Protein ◽  
The Czech Republic ◽  
Protein Constructs

scholarly journals Genetic and biological characterisation of a Grapevine virus A isolate from the Czech Republic

2009 ◽  
Vol 44 (No. 4) ◽  
pp. 121-126
Author(s):  
P. Komínek ◽  
M. Komínková
Keyword(s):  
Czech Republic ◽  
Coat Protein ◽  
Genomic Sequence ◽  
Movement Protein ◽  
Amino Acid Identity ◽  
Grapevine Virus ◽  
The Czech Republic ◽  
Grapevine Cultivar ◽  
Grapevine Virus A ◽  
Kober Stem Grooving

An isolate of <i>Grapevine virus A</i> (GVA) from the Czech Republic was obtained from the grapevine cultivar Müller Thurgau. Symptoms of GVA – Kober stem grooving disease were not observed in the infected grapevines (which had been grafted onto Kober 5BB rootstock). A partial genomic sequence of the GVA isolate, 1523 nucleotides long, was obtained. The sequence completely covers the genes for both a movement and a coat protein. Compared to the GVA sequences available in databases, the nucleotide identity reached 84%. The amino acid identity in the movement protein reached 88%, and 98% in the coat protein.

scholarly journals First Report of Barley yellow dwarf virus-PAS in Wheat and Barley Grown in the Czech Republic

Plant Disease ◽  
2008 ◽  
Vol 92 (11) ◽  
pp. 1587-1587 ◽  
Author(s):  
J. K. Kundu
Keyword(s):  
Czech Republic ◽  
Amino Acid ◽  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Yellow Dwarf ◽  
Cereal Crops ◽  
The Czech Republic ◽  
Barley Yellow Dwarf ◽  
Yellow Dwarf Virus

Barley yellow dwarf disease, an important, ubiquitous virus disease of cereal crops worldwide, is caused by a group of related single-stranded RNA viruses assigned to luteovirus (Barley yellow dwarf virus (BYDV) spp. PAV, PAS, MAV, and GAV) or polerovirus (Cereal yellow dwarf virus-RPV) genera or unassigned to a genera (BYDV-SGV, BYDV-RMV, and BYDV-GPV) in the family Luteoviridae (2). Incidence of BYDV in cereal crops (e.g., barley, wheat, and oats) was high and reached epidemic levels in recent years in many regions of the Czech Republic. Previously, only PAV isolates have been identified here on the basis of serological detection (4), although antibodies to differentiate between PAV, PAS, and MAV are not widely available. Field samples of cereal crops were routinely tested in 2006 and 2007 and BYDVs were detected by ELISA. One-step-reverse transcription (RT)-PCR (Qiagen, Hilden, Germany) was adapted for BYDV detection using primer pairs BYcpF (5′-CCACTAGAGAGGTGGTGAATG-3′) and BYcpR (5′-CCGGTGTTGAGGAGTCTACC-3′) designed from conserved sequences identified by aligning multiple BYDV sequences available in public databases. These primers amplify a 641-bp fragment spanning nucleotides 2839–3479 from PAV (GenBank Accession No. EF043235) or PAS (GenBank Accession No. NC_002160) that includes a region of the coat protein gene and the intergenic region. RT-PCR amplicons were generated from two field isolates, PS-RuJK (spring wheat isolate, cv. Granny, collected in July 2007 from experimental plots at the CRI in Prague) and JE-120JK (winter barley isolate, cv. Merlot, collected in January 2008 from a barley field in Rychnov), both of which induced severe BYD symptoms. Amplicons were sequenced in both directions in a CEQ2000XL sequencer (Beckman Coulter, Fullerton, CA). The partial coat protein gene sequence of 483 nt of PS-RuJK and JE-120JK was analyzed and compared with available sequences of 26 PAV, 17 PAS, and 13 MAV isolates by MEGA4 (3). PS-RuJK (GenBank Accession No. EU863652) nucleotide and amino acid sequence identities ranged from 96.3 to 99.2% and 93.7 to 98.7%, respectively, for available PAS isolates, and 89.9 to 90.5% and 85.5 to 86.9%, respectively, for available PAV isolates, and 78.3 to 79.5% and 70.0 to 72.5%, respectively, for available MAV isolates. Similarly, nucleotide and amino acid sequence identities JE-120JK (GenBank Accession No. EU863653) ranged from 95.2 to 98.6% and 90.6 to 96.9%, respectively, for PAS isolates, 88.8 to 90.1% and 83.1 to 84.4%, respectively, for PAV isolates, and 77.6 to 78.7% and 67.5 to 70.0%, respectively, for MAV isolates. Also, both of these isolates have the conserved amino acid motif “SIPGS” that is usually present in a variable region of the coat protein gene on the surface of virion (1) at position 52 to 56 of amino acid sequences of all published PAS-like isolates, including Vd29:AY167109, FH1:AJ223588, MA9516:AJ007926, FL2:AJ223586, ASL-1:AJ810418, and WS6603:DQ285680, contrary to “PVFRP” or “LISGP” motif in PAV or MAV, respectively. Therefore, the sequence data clearly confirm that these two isolates belong to the PAS species. To our knowledge, this is the first record of PAS detected in the Czech Republic. References: (1) C. A. Chay et al. Phytopathology 86:370, 1996. (2) C. J. D'Arcy and L. L. Domier. Page 891 in: Virus Taxonomy-8th Report of the ICTV. C. M. Fauquet et al., eds. Springer-Verlag, NY, 2005. (3) K. Tamura et al. Mol. Biol. Evol. 24:1596, 2007. (4) J. Vacke. Page 100 in: Sbornik Referatu z Odborneho Seminare, Aktualni Problemy Ochrany Polnich Plodin. Praha, 1991.

Social variation in size at birth and preterm delivery in the Czech Republic and Sweden, 1989-91

1998 ◽  
Vol 12 (1) ◽  
pp. 7-24 ◽  
Author(s):  
Koupilova ◽  
Vagero ◽  
Leon ◽  
Pikhart ◽  
Prikazsky ◽  
...  
Keyword(s):  
Czech Republic ◽  
Preterm Delivery ◽  
The Czech Republic ◽  
Social Variation ◽  
Size At Birth

On Geropsychology in the Czech Republic

GeroPsych ◽  
2012 ◽  
Vol 25 (3) ◽  
pp. 161-166
Author(s):  
Hana Stepankova ◽  
Eva Jarolimova ◽  
Eva Dragomirecka ◽  
Irena Sobotkova ◽  
Lenka Sulova ◽  
...  
Keyword(s):  
Czech Republic ◽  
Clinical Practice ◽  
Old Age ◽  
Cognitive Rehabilitation ◽  
The Czech Republic ◽  
Rehabilitation Research

This work provides an overview of psychology of aging and old age in the Czech Republic. Historical roots as well as recent activities are listed including clinical practice, cognitive rehabilitation, research, and the teaching of geropsychology.

Contemporary Psychology in the Czech Republic

2000 ◽  
Vol 5 (1) ◽  
pp. 62-65 ◽  
Author(s):  
Ji&rcaron;í Hoskovec ◽  
Josef M. Brožek
Keyword(s):  
Czech Republic ◽  
The Czech Republic

Notes on the Oak-hornbeam forest communitier of the Czech Republic

1994 ◽  
Vol 105 (7-8) ◽  
pp. 481-497
Author(s):  
Z. Neuhäuslová ◽  
J. Kolbek
Keyword(s):  
Czech Republic ◽  
The Czech Republic
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