Effects of over-expression of allene oxide cyclase on camptothecin production by cell cultures of Camptotheca acuminata

2012 ◽  
Vol 11 (24) ◽  
Author(s):  
Yan Pi
Keyword(s):  
Cell Cultures ◽  
Camptotheca Acuminata ◽  
Allene Oxide ◽  
Allene Oxide Cyclase ◽  
Over Expression

Molecular cloning, characterization and expression of a jasmonate biosynthetic pathway gene encoding allene oxide cyclase from Camptotheca acuminata

2008 ◽  
Vol 28 (6) ◽  
pp. 349-355 ◽  
Author(s):  
Yan Pi ◽  
Zhihua Liao ◽  
Keji Jiang ◽  
Beibei Huang ◽  
Zhongxiang Deng ◽  
...  
Keyword(s):  
Plant Species ◽  
Genomic Dna ◽  
Amino Acid Level ◽  
Camptotheca Acuminata ◽  
Pcr Analysis ◽  
Allene Oxide ◽  
Allene Oxide Cyclase ◽  
Significant Similarity ◽  
Real Time Quantitative Pcr ◽  
Pathway Gene

AOC (allene oxide cyclase; EC 5.3.99.6), an essential enzyme in jasmonic acid and its methyl ester biosynthesis, was cloned from Camptotheca acuminata (named as CaAOC), a native medicinal plant species in China. CaAOC had significant similarity at the amino-acid level with AOCs from other plant species. Comparison between the sequences of the full-length cDNA and genomic DNA of CaAOC revealed that the genomic DNA of CaAOC contained an 89-bp intron and a 240-bp intron. Southern-blot analysis indicated that CaAOC was a multiple-copy gene, and real-time quantitative PCR analysis showed that CaAOC was expressed constitutively in all organs tested, with the highest expression level in leaves. The results from treatment experiments using different signalling components, including methyl jasmonate, abscisic acid, salicylic acid and H2O2, revealed that expression of CaAOC had a prominent diversity. Heavy metal (copper) significantly enhanced CaAOC expression, whereas wounding (induced by UV-B) was not so effective.

Allene Oxide Cyclase from Camptotheca acuminata Improves Tolerance Against Low Temperature and Salt Stress in Tobacco and Bacteria

2008 ◽  
Vol 41 (2) ◽  
pp. 115-122 ◽  
Author(s):  
Yan Pi ◽  
Keji Jiang ◽  
Ying Cao ◽  
Qian Wang ◽  
Zhuoshi Huang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Low Temperature ◽  
Camptotheca Acuminata ◽  
Allene Oxide ◽  
Allene Oxide Cyclase

The effect of growth regulators and sucrose on anthocyanin production in Camptotheca acuminata cell cultures

2005 ◽  
Vol 43 (3) ◽  
pp. 293-298 ◽  
Author(s):  
Gabriella Pasqua ◽  
Barbara Monacelli ◽  
Nadia Mulinacci ◽  
Simona Rinaldi ◽  
Catia Giaccherini ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Growth Regulators ◽  
Camptotheca Acuminata ◽  
Anthocyanin Production

scholarly journals Allene oxide cyclase dependence of the wound response and vascular bundle-specific generation of jasmonates in tomato - amplification in wound signalling

2003 ◽  
Vol 33 (3) ◽  
pp. 577-589 ◽  
Author(s):  
Irene Stenzel ◽  
Bettina Hause ◽  
Helmut Maucher ◽  
Andrea Pitzschke ◽  
Otto Miersch ◽  
...  
Keyword(s):  
Vascular Bundle ◽  
Wound Response ◽  
Allene Oxide ◽  
Allene Oxide Cyclase

scholarly journals Effects of over-expression of strictosidine synthase and tryptophan decarboxylase on alkaloid production by cell cultures of Catharanthus roseus

Planta ◽  
1998 ◽  
Vol 205 (3) ◽  
pp. 414-419 ◽  
Author(s):  
Camilo Canel ◽  
M. Inês Lopes-Cardoso ◽  
Serap Whitmer ◽  
Leslie van der Fits ◽  
Giancarlo Pasquali ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Catharanthus Roseus ◽  
Tryptophan Decarboxylase ◽  
Alkaloid Production ◽  
Strictosidine Synthase ◽  
Over Expression

scholarly journals A Wheat Allene Oxide Cyclase Gene Enhances Salinity Tolerance via Jasmonate Signaling

2013 ◽  
Vol 164 (2) ◽  
pp. 1068-1076 ◽  
Author(s):  
Yang Zhao ◽  
Wei Dong ◽  
Naibo Zhang ◽  
Xinghui Ai ◽  
Mengcheng Wang ◽  
...  
Keyword(s):  
Salinity Tolerance ◽  
Allene Oxide ◽  
Allene Oxide Cyclase ◽  
Jasmonate Signaling

Allene-oxide cyclase

1998 ◽  
pp. 255-257
Author(s):  
Dietmar Schomburg ◽  
Dörte Stephan
Keyword(s):  
Allene Oxide ◽  
Allene Oxide Cyclase

ScAOC1, an allene oxide cyclase gene, confers defense response to biotic and abiotic stresses in sugarcane

2020 ◽  
Vol 39 (12) ◽  
pp. 1785-1801
Author(s):  
Tingting Sun ◽  
Guangli Cen ◽  
Chuihuai You ◽  
Wenyue Lou ◽  
Zhoutao Wang ◽  
...  
Keyword(s):  
Defense Response ◽  
Abiotic Stresses ◽  
Allene Oxide ◽  
Biotic And Abiotic Stresses ◽  
Allene Oxide Cyclase

scholarly journals Comparative Proteomic Analysis of Dipsacus asperoides Roots from Different Habitats in China

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3605
Author(s):  
Haijun Jin ◽  
Hua Yu ◽  
Haixia Wang ◽  
Jia Zhang
Keyword(s):  
Proteomic Analysis ◽  
Absolute Quantification ◽  
Pcr Analysis ◽  
Allene Oxide ◽  
Allene Oxide Cyclase ◽  
Protein Levels ◽  
Depth Analysis ◽  
Differential Proteins ◽  
Isobaric Tags

Dipsacus asperoides is a kind of Chinese herbal medicine with beneficial health properties. To date, the quality of D. asperoides from different habitats has shown significant differences. However, the molecular differences in D. asperoides from different habitats are still unknown. The aim of this study was to investigate the differences in protein levels of D. asperoides from different habitats. Isobaric tags for relative and absolute quantification (iTRAQ) and 2DLC/MS/MS were used to detect statistically significant changes in D. asperoides from different habitats. Through proteomic analysis, a total of 2149 proteins were identified, of which 42 important differentially expressed proteins were screened. Through in-depth analysis of differential proteins, the protein metabolism energy and carbohydrate metabolism of D. asperoides from Hubei Province were strong, but their antioxidant capacity was weak. We found that three proteins, UTP-glucose-1-phosphate uridylyltransferase, allene oxide cyclase, and isopentyl diphosphate isomerase 2, may be the key proteins involved in dipsacus saponin VI synthesis. Eight proteins were found in D. asperoides in response to environmental stress from different habitats. Quantitative real-time PCR analysis confirmed the accuracy and authenticity of the proteomic analysis. The results of this study may provide the basic information for exploring the cause of differences in secondary metabolites in different habitats of D. asperoides and the protein mechanism governing differences in quality.

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