Study of in vitro degradation of biodegradable polymer based thin films and tissue engineering scaffolds

2011 ◽  
Vol 10 (81) ◽  
Author(s):  
Naznin Sultana
Keyword(s):  
Thin Films ◽  
Tissue Engineering ◽  
Biodegradable Polymer ◽  
Tissue Engineering Scaffolds ◽  
In Vitro Degradation

Characterisation of ‘wet’ polymer surfaces for tissue engineering applications: Are flat surfaces a suitable model for complex structures?

e-Polymers ◽  
2005 ◽  
Vol 5 (1) ◽  
Author(s):  
Laleh Safinia ◽  
Jonny J. Blaker ◽  
Véronique Maquet ◽  
Aldo R. Boccaccini ◽  
Athanassios Mantalaris ◽  
...  
Keyword(s):  
Thin Films ◽  
Tissue Engineering ◽  
Pore Wall ◽  
Polymer Surfaces ◽  
Suitable Model ◽  
Tissue Engineering Scaffolds ◽  
3D Structures ◽  
Flat Surfaces ◽  
Point Test ◽  
Highly Porous

AbstractTissue engineering scaffolds are 3D constructs that simulate the growth environment in vivo. The present work aims to address the question of whether thin films, i.e., flat surfaces, are a suitable model for more complex 3D structures? With this in mind a complete study of the morphology and surface chemistry of poly(D,Llactide) (PDLLA) substrates, fabricated into two different structures, is presented. The polymer structures studied include a 3D, porous, foam-like scaffold prepared by the thermally induced phase separation (TIPS) method and flat polymer thin films made by solvent casting. Based on the maximum bubble point test, a new method to assess the wettability of wet pore wall surfaces inside highly porous 3D structures was developed and tested. The maximum pore diameter determined using the maximum bubble point test for the total wetting liquids was confirmed through image analysis of scanning electron micrographs. The method allows the determination of the contact angle between the wet pore wall and a contacting liquid. The captive bubble method was employed to characterise the wettability of flat polymer films in contact with water. Both structures were further characterised using zeta- (ζ-) potential measurements to assess the surface chemistry of the polymer. The results demonstrate that PDLLA contains acidic functional groups and is hydrophobic. In order to evaluate the sensitivity of the test methods, the polymer surfaces were modified by protein adsorption using fibronectin and collagen. ζ-Potential and wettability measurements show that proteins indeed adsorb on virgin PDLLA surfaces. Protein adsorption causes the wettability of the PDLLA for water to improve. Our results strongly indicate that flat surfaces are not a suitable model for surfaces in complex 3D structures such as highly porous tissue engineering scaffolds. Such scaffolds must be characterised as a 3D system.

Preparation, Characterization and In Vitro Release Kinetics of Vancomycin Carried β-TCP/Chitosan Composite Microspheres Used as Bone Tissue Engineering Scaffolds

2012 ◽  
Vol 512-515 ◽  
pp. 1821-1825
Author(s):  
Lin Zhang ◽  
Xue Min Cui ◽  
Qing Feng Zan ◽  
Li Min Dong ◽  
Chen Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Release Kinetics ◽  
In Vitro Release ◽  
Cross Linking ◽  
Tissue Engineering Scaffolds ◽  
Composite Microspheres ◽  
Chitosan Composite ◽  
Vitro Release

A novel microsphere scaffolds composed of chitosan and β-TCP containing vancomycin was designed and prepared. The β-TCP/chitosan composite microspheres were prepared by solid-in-water-in-oil (s/w/o) emulsion cross-linking method with or without pre-cross-linking process. The mode of vancomycin maintaining in the β-TCP/chitosan composite microspheres was detected by Fourier transform infrared spectroscopy (FTIR). The in vitro release curve of vancomycin in simulated body fluid (SBF) was estimated. The results revealed that the pre-cross-linking prepared microspheres possessed higher loading efficiency (LE) and encapsulation efficiency (EE) especially decreasing the previous burst mass of vancomycin in incipient release. These composite microspheres got excellent sphere and well surface roughness in morphology. Vancomycin was encapsulated in composite microspheres through absorption and cross-linking. While in-vitro release curves illustrated that vancomycin release depond on diffusing firstly and then on the degradation ratio later. The microspheres loading with vancomycin would be to restore bone defect, meanwhile to inhibit bacterium proliferation. These bioactive, degradable composite microspheres have potential applications in 3D tissue engineering of bone and other tissues in vitro and in vivo.

Tissue Engineering Scaffolds Based on Photocured Dimethacrylate Polymers for in Vitro Optical Imaging

2006 ◽  
Vol 7 (6) ◽  
pp. 1751-1757 ◽  
Author(s):  
Forrest A. Landis ◽  
Jean S. Stephens ◽  
James A. Cooper ◽  
Marcus T. Cicerone ◽  
Sheng Lin-Gibson
Keyword(s):  
Tissue Engineering ◽  
Optical Imaging ◽  
Tissue Engineering Scaffolds

scholarly journals Three-Dimensional Microfluidic Tissue-Engineering Scaffolds Using a Flexible Biodegradable Polymer

2006 ◽  
Vol 18 (2) ◽  
pp. 165-169 ◽  
Author(s):  
C. J. Bettinger ◽  
E. J. Weinberg ◽  
K. M. Kulig ◽  
J. P. Vacanti ◽  
Y. Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Biodegradable Polymer ◽  
Three Dimensional ◽  
Tissue Engineering Scaffolds

Mineralized Nanofibers for Bone Tissue Engineering

2018 ◽  
pp. 461-475 ◽  
Author(s):  
Ozan Karaman
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Bone Grafts ◽  
Tissue Engineering Scaffolds ◽  
Promising Alternative ◽  
Biomimetic Scaffolds

The limitation of orthopedic fractures and large bone defects treatments has brought the focus on fabricating bone grafts that could enhance ostegenesis and vascularization in-vitro. Developing biomimetic materials such as mineralized nanofibers that can provide three-dimensional templates of the natural bone extracellular-matrix is one of the most promising alternative for bone regeneration. Understanding the interactions between the structure of the scaffolds and cells and therefore the control cellular pathways are critical for developing functional bone grafts. In order to enhance bone regeneration, the engineered scaffold needs to mimic the characteristics of composite bone ECM. This chapter reviews the fabrication of and fabrication techniques for fabricating biomimetic bone tissue engineering scaffolds. In addition, the chapter covers design criteria for developing the scaffolds and examples of enhanced osteogenic differentiation outcomes by fabricating biomimetic scaffolds.

scholarly journals Development of 3D Bioactive Nanocomposite Scaffolds Made from Gelatin and Nano Bioactive Glass for Biomedical Applications

2010 ◽  
Vol 19 (2) ◽  
pp. 096369351001900 ◽  
Author(s):  
M. Mozafari ◽  
F. Moztarzadeh ◽  
M. Rabiee ◽  
M. Azami ◽  
N. Nezafati ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Biomedical Applications ◽  
Cell Attachment ◽  
Tissue Engineering Scaffolds ◽  
Study Results ◽  
Nanocomposite Scaffold ◽  
Nanocomposite Scaffolds ◽  
First Time

In this research, macroporous, mechanically competent and bioactive nanocomposite scaffolds have been fabricated from cross-linked gelatine (Gel) and nano bioactive glass (nBG) through layer solvent casting combined with freeze-drying and lamination techniques. This study has developed a new composition to produce a new bioactive nanocomposite which is porous with interconnected microstructure, pore sizes are 200-500 μm, porosity are 72%-86%. Also, we have reported formation of chemical bonds between nBG and Gel for the first time. Finally, the in vitro cytocompatability of the scaffolds was assessed using MTT assay and cell attachment study. Results indicated no sign of toxicity and cells found to be attached to the pore walls offered by the scaffolds. These results suggested that the developed nanocomposite scaffold possess the prerequisites for bone tissue engineering scaffolds and it can be used for tissue engineering applications.

scholarly journals Feasibility of Spatially Offset Raman Spectroscopy for in Vitro and in Vivo Monitoring Mineralization of Bone Tissue Engineering Scaffolds

2016 ◽  
Vol 89 (1) ◽  
pp. 847-853 ◽  
Author(s):  
Zhiyu Liao ◽  
Faris Sinjab ◽  
Amy Nommeots-Nomm ◽  
Julian Jones ◽  
Laura Ruiz-Cantu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Raman Spectroscopy ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Tissue Engineering Scaffolds ◽  
In Vivo Monitoring
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