Comparative analysis of vitamin C, crude protein, elemental nitrogen and mineral content of canola greens (Brassica napus L.) and kale (Brassica oleracea var. acephala)

2011 ◽  
Vol 10 (83) ◽  
Author(s):  
Funda Eryilmaz Acikgoz
2011 ◽  
Vol 42 (No. 3) ◽  
pp. 103-110 ◽  
Author(s):  
N.D. Kaur ◽  
M. Vyvadilová ◽  
M. Klíma ◽  
M. Bechyně

An improved protocol for Brassica protoplast culture and plant regeneration was developed. Isolated protoplasts from four-weeks-old in vitro shoot tip culture of Brassica oleracea var. botrytis cv. Siria F1 and Brassica napus doubled haploid of breeding line OP-1 were cultured at a density of 9.8&ndash;11.2 &times; 10<sup>4 </sup>protoplasts/ml in darkness at 25&deg;C in a modified medium containing 2% glucose, 0.25 mg/l 2,4-D, 1 mg/l BAP and 1 mg/l NAA. The first divisions of protoplasts were observed on the third day of culture in B. oleracea and on the fourth day in B. napus. The protoplast cultures were diluted with low osmotic medium on 7<sup>th</sup> and 11<sup>th</sup> day. The frequency of dividing cells was about 80% in B. oleracea and 50% in B. napus. After one month, the microcalli of approximately 0.5&ndash;1 mm in size were transferred into an induction medium with various combinations of growth regulators. Minimum duration of enzyme treatment time and extended dark period in the initial phase of culture increased the survival rate of protoplasts. Organogenesis started when the calli enlarged in size on an induction medium (1 mg/l NAA, 0.02 mg/l GA<sub>3</sub>, 1 mg/l 2iP) with 2% sucrose and 0.8% agar. Regeneration frequency of calli was found to be 69&ndash;75% in B. oleracea and 2&ndash;3% in B. napus. Well-developed shoots were transferred for rooting to a half-strength MS medium without growth regulators. More than 100 B. oleracea regenerants were transferred into soil, and they produced normal heads and set seeds. This very simple procedure is efficient and suitable mainly for B. oleracea var. botrytis and represents a background for fusion experiments. &nbsp;


2008 ◽  
Vol 59 (6) ◽  
pp. 578 ◽  
Author(s):  
J. L. Jacobs ◽  
G. N. Ward ◽  
Gavin Kearney

In southern Australia, the majority of dry land dairy farms use a 2-pond system to treat and contain dairy effluent collected at the milking platform. This effluent contains a range of nutrients that have the potential to affect forage dry matter (DM) yields, nutritive characteristics, and mineral content of forages. The effect of applying second-pond dairy effluent to the summer-active forages chicory (Chichorium intybus L. cv. Grouse), Hunter (Brassica campestris L. × Brassica napus L.), Winfred (Brassica napus L.), and Sweet Jumbo (Sorghum bicolor (L.) Moench × Sorghum sudanese (Piper) Stapf.) over two summer periods was measured. Effluent was applied at rates of 0, 40, 80, and 100 mm with application split into two equal application times. The first occurred 6–10 weeks after sowing and the second immediately after the first grazing. Forages were assessed for DM yield, nutritive characteristics, and mineral content over the two growth periods in each year. Analysis of effluent showed that on average over the 2 years, the effluent contained 146, 34, 439, and 18 kg/ML of nitrogen (N), phosphorus (P), potassium (K), and sulfur (S), respectively. Furthermore, the effluent also contained 161 kg/ML of calcium (Ca) and 222 kg/ML of magnesium (Mg). For the total growth period in Year 1, all crops showed a linear increase (P < 0.05) in DM yield to applied effluent, with response values varying for each crop. Responses were 49, 52, 29, and 51 kg DM/ha.mm applied effluent for chicory, Hunter, Sweet Jumbo, and Winfred, respectively. For Year 2, all crops also showed a linear increase (P < 0.05) in DM yield (15 kg DM/ha.mm applied effluent) with applied effluent. In Year 1, crude protein (CP) content of all crops increased (P < 0.05) in a linear manner at rates of 0.073 and 0.047% per mm applied effluent for growth periods 1 and 2. There were also linear responses (P < 0.05) in Year 2, with responses varying for each crop for each growth period. For chicory there was no effect of effluent application on CP content in either growth period, while other crops generally exhibited a linear increase with responses of up to 0.08% per mm applied effluent. The greatest changes in mineral content of herbage were those of K, Ca, and Mg. There was a linear increase (P < 0.05) in K content for all growth periods in Years 1 and 2. Magnesium content of chicory (periods 1 and 2) and Winfred (period 2) showed a linear decrease (P < 0.05) in response to effluent application in Year 1, whereas there was no effect in Year 2 for any crops. The results from this study highlight the potential of second-pond dairy effluent to increase DM yields of a range of summer-active forage crops. The data also suggest that while effluent can improve DM yields when soil moisture is limiting, when additional moisture as rainfall occurs, responses from effluent are even more pronounced. In addition, the CP content of forages can be improved when effluent is applied. The combination of increased DM yield with higher CP content provides greater flexibility in dairy cattle feeding options through the dry summer period.


2016 ◽  
Vol 36 (9) ◽  
Author(s):  
Yan Long ◽  
Jing Wang ◽  
Yanyan Wang ◽  
Jinwen Zhang ◽  
Jiao Wang ◽  
...  

Nativa ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 600-604
Author(s):  
Anderson Bergamasco Hryczyna ◽  
Tiago Roque Benetoli da Silva ◽  
Lucas Ambrosano ◽  
Gesmila Karoline Zampronio

A canola (Brassica napus L. var oleífera) é uma oleaginosa, pertencente à família Brassicaceae, resultado do cruzamento interespecífico entre repolho silvestre (Brassica oleracea L.) e mostarda (Brassica rapa L., syn. campestris). O boro é considerado um micronutriente, que atua na translocação de açúcares, formação de proteínas, crescimento do tubo polínico, germinação dos grãos de pólen, desenvolvimento das sementes e das paredes celulares. O objetivo desta pesquisa foi estudar as interferências nos componentes produtivos de dois híbridos de canola com a aplicação de adubação com boro por via foliar. Para tanto, instalou-se um experimento em condições de campo num delineamento experimental de blocos ao acaso em arranjo fatorial 2 x 3. Utilizou-se os híbridos Hyola 433 e Hyola 575 CL e três doses de boro foliar (0;100; 200) g ha-1 de B. As seguintes características foram avaliadas:  número de plantas, número de síliquas por planta, massa de parte aérea, massa de 100 grãos e produtividade. Com a aplicação de boro houve aumento da massa de grãos independente do híbrido. Para produtividade houve aumento somente para o híbrido Hyola 433, sendo a maior produtividade alcançada na dose de 200 g ha-1. O híbrido Hyola 575 CL não teve sua produtividade alterada com a aplicação de boro. Palavras-chave: ácido bórico; Brassica napus L. var. oleífera; colza; massa de 100 grãos; oleaginosa.   Leaf boron fertilization in two rapessed   ABSTRACT: Rapessed (Brassica napus L. var oleífera) is an oilseed, belongs to Brassicaceae family, resultated between inter specific crossing of wild cabbage (Brassica oleracea L.) and mustard (Brassica rapa L., syn. campestris). Boron is considered a micronutrient, acting in sugars translocation, protein formation, pollen tube growth, pollen grain germination, seeds and cell wall development. The aimed was to study the interferences in productive components of two rapessed hybrids with application of boron fertilization by leaf application. For this, a field experiment was installed by using experimental design of randomized blocks in a 2 x 3 factorial scheme.  Hyola 433 and Hyola 575CL hybrids and three doses of leaf boron (0; 100 and 200) g ha-1 of B were used. The following characteristics were evaluated: plants number, number of pods per plant, mass of one hundred grains and yield. With boron application there was an increase in grain mass independent of the hybrid. In relation grain yield there was increase only for Hyola 433 hybrid, with the highest yield reached at 200 g ha-1. The Hyola 575CL hybrid did not have its productivity altered with the application of boron. Keywords: boric acid; Brassica napus L. var. oleífera; mustard; mass of a hundred grass; oilseeds.


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