Effects of sublethal doses of chlorfluazuron on the ovarian biochemical constituents of Spodoptera litura

2011 ◽  
Vol 10 (62) ◽  
Author(s):  
F. Perveen
2011 ◽  
Vol 143 (2) ◽  
pp. 178-184
Author(s):  
F. Perveen

AbstractThe effects of sublethal doses (LD10: 1.00 ng/larva; LD30: 3.75 ng/larva) of chlorfluazuron on the biochemical constituents of eggs of the tobacco cutworm, Spodoptera litura (F.), are described. Chlorfluazuron was applied topically to fifth-instar larvae and the subsequent adults were allowed to mate according to larval treatment (LD10-treated female × LD10-treated male and LD30-treated female × LD30-treated male). Biochemical constituents of eggs resulting from these pairings were analyzed at various stages of embryonic development. Compared with controls, LD10 or LD30 reduced egg constituents as follows: protein (min.–max.) by 32.8%–34.5% or 62.0%–67.3%, lipid by 33%–34% or 62%–67%, carbohydrates by 30%–39% or 60%–67%, DNA by 33%–40% or 60%–69%, RNA by 31%–34% or 59%–67%, and ecdysteroid by 22%–83% or 28%–92%, respectively. The relative proportions of constituents in control eggs were as follows: protein > lipid > carbohydrate, and RNA > DNA. Three low and three high peaks in ecdysteroid titres were observed. Compared with controls, all peaks were reduced in LD10 or LD30 eggs as follows: low peaks: 1st (at 8 h): 32% or 66%; 2nd (at 16 h): 33% or 67%; 3rd (at 52 h): 35% or 65%; high peaks: 1st (at 32 h): 83% or 92%; 2nd (at 64 h): 65% or 82%; 3rd (at 84 h): 84 h, 36% or 63%, respectively. In addition, the first two high peaks were delayed by 4 h in LD10 eggs and by 8 h in LD30 eggs compared with controls. Sublethal doses of chlorfluazuron reduced the amounts of biochemical constituents of eggs during embryogenesis in S. litura.


ENTOMON ◽  
2019 ◽  
Vol 44 (2) ◽  
pp. 127-132
Author(s):  
M. Visnupriya ◽  
N. Muthukrishnan

Field population of Spodoptera litura from tomato ( resistant to the majority of the conventional insecticide molecules) were subjected to the in vivo toxicity of spinetoram 12 SC to assess whether cross resistance exists or not. Untreated larvae of both field and laboratory strains showed no mortality during 48 hours of feeding. After 48 hours of feeding on spinetoram 12 SC treated leaves, LC50s of field larvae were 0.28, 0.93, 3.71 and 7.11 ppm for the 2nd, 3rd, 4th and 5th instars of S. litura respectively. However, in the laboratory strain these values were 1.12, 5.86, 36.72 and 91.55 ppm for 2nd, 3rd, 4th and 5th instars of S. litura respectively. Resistance ratio was 0.25, 0.16, 0.10 and 0.08 for the 2nd instar up to the 5th instar of S. litura.


Author(s):  
Irma Harlianingtyas ◽  
◽  
Ramadhan Taufika ◽  

Tembakau adalah produk pertanian semusim yang bukan termasuk dalam komoditas perkebunan. Produk ini dikonsumsi bukan untuk makanan tetapi sebagai pengisi waktu luang yaitu sebagai bahan baku rokok dan cerutu. Tembakau juga dapat dikunyah. Kandungan metabolit sekunder yang kaya juga membuatnya bermanfaat sebagai pestisida dan bahan baku obat. Salah satu tantangan dalam budidaya tembakau adalah adanya organisme pengganggu tanaman. Serangan hama dan penyakit dapat mempengaruhi kualitas maupun kuantitas hasil panen tembakau. Serangan hama dan penyakit yang paling banyak terjadi adalah pada saat fase vegetatif, karena pada vase ini semua bagian tanaman menjadi sasaran makanan bagi organisme pengganggu tanaman. Organisme pengganggu tanaman yang dianggap sebagai hama pada vase vegetattif tanaman tembakau adalah ualt tanah, ulat daun, belalang, kupu, gayas/jangkrik. Kegiatan penelitian ini dilakukan di PT Tarutama Nusantara Jember, data yang digunakan adalah data serangan hama yang terjadi pada seluruh kebun milik TTN dari tahun 2013 hingga 2019. Tujuan dilakukan penelitian ini adalah mengtahui prediksi serangan hama yang akan terjadi guna mempermudah perusahaan dalam menentukan strategi dan kebijakan guna meningkatkan produksi tembakau khususnya sebagai pelapis cerutu dekblad/wrapper. Metode penelitian yang digunakan untuk memprediksi serangan hama yang terjadi adalah metode regresi linier. Hasil penelitian menunjukkan prediksi serangan hama ulat tanah (Agrotis iplison) tahun 2021 sebesar 15,6%, ulat grayak (Spodoptera litura) sebesar 19,08%, serangan hama belalang sebesar 15,77%, dan serangan hama ketep (Setomorpha rutella) sebesar 15,23%, serta serangan hama jangkrik (Gryllidae) pada tahun 2021 adalah sebesar 8,20%.


2010 ◽  
Vol 9 (1) ◽  
pp. 133-140
Author(s):  
Petrisor Zamora Iordache ◽  
Nicoleta Petrea ◽  
Vasile Somoghi ◽  
Mihaela Muresan ◽  
Gabriel Epure ◽  
...  

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