Construction of a fusion gene containing hepatitis B virus L gene and Mycobacterium tuberculosis Ag85B gene and its expression in Pichia pastoris

Li Junwu; Gong Junyuan; Liu Xin; Ye Qiuping; Song Dong; Xiao Renjie

doi:10.5897/ajb11.190

Construction of a fusion gene containing hepatitis B virus L gene and Mycobacterium tuberculosis Ag85B gene and its expression in Pichia pastoris

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb11.190 ◽

2011 ◽

Vol 10 (60) ◽

pp. 12840-12846

Author(s):

Li Junwu ◽

Gong Junyuan ◽

Liu Xin ◽

Ye Qiuping ◽

Song Dong ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Hepatitis B Virus ◽

Pichia Pastoris ◽

Hepatitis B ◽

Fusion Gene ◽

B Virus

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Prophylactic antiviral treatment reduces the incidence of liver failure among patients coinfected with Mycobacterium tuberculosis and hepatitis B virus

Virus Research ◽

10.1016/j.virusres.2019.197664 ◽

2019 ◽

Vol 270 ◽

pp. 197664

Author(s):

Jiangshan Lian ◽

Ping Hu ◽

Yingfeng Lu ◽

Yueying Liu ◽

Xiaoxiao Wang ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Hepatitis B Virus ◽

Hepatitis B ◽

Liver Failure ◽

Antiviral Treatment ◽

B Virus

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Expression, purification and characterization of the Hepatitis B virus entire envelope large protein in Pichia pastoris

Protein Expression and Purification ◽

10.1016/j.pep.2006.05.002 ◽

2006 ◽

Vol 49 (2) ◽

pp. 168-175 ◽

Cited By ~ 10

Author(s):

Xue Han ◽

Lin-Bai Ye ◽

Bao-zong Li ◽

Gao Bo ◽

Wei-jia Cai ◽

...

Keyword(s):

Hepatitis B Virus ◽

Pichia Pastoris ◽

Hepatitis B ◽

Large Protein ◽

Purification And Characterization ◽

B Virus

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Acid-fast bacterium-Escherichia coli shuttle vector; gene cloning and expression in e.g. Mycobacterium bovis BCG for multivalent recombinant vaccine production, e.g. against Mycobacterium tuberculosis or hepatitis B virus

Vaccine ◽

10.1016/0264-410x(93)90391-a ◽

1993 ◽

Vol 11 (9) ◽

pp. 981

Keyword(s):

Escherichia Coli ◽

Mycobacterium Tuberculosis ◽

Hepatitis B Virus ◽

Hepatitis B ◽

Mycobacterium Bovis ◽

Shuttle Vector ◽

Cloning And Expression ◽

Gene Cloning And Expression ◽

Vaccine Production ◽

B Virus

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Expression, Purification, and Characterization of Hepatitis B Virus Surface Antigens (HBsAg) in Yeast Pichia Pastoris

Applied Biochemistry and Biotechnology ◽

10.1007/s12010-009-8527-x ◽

2009 ◽

Vol 158 (2) ◽

pp. 432-444 ◽

Cited By ~ 19

Author(s):

Rushi Liu ◽

Qinlu Lin ◽

Yi Sun ◽

Xiangyang Lu ◽

Yilan Qiu ◽

...

Keyword(s):

Hepatitis B Virus ◽

Pichia Pastoris ◽

Hepatitis B ◽

Surface Antigens ◽

Purification And Characterization ◽

Virus Surface ◽

Yeast Pichia Pastoris ◽

B Virus

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Expression of Hepatitis B Virus S Gene in Pichia pastoris and Application of the Product for Detection of Anti-HBs Antibody

BMB Reports ◽

10.5483/bmbrep.2005.38.6.683 ◽

2005 ◽

Vol 38 (6) ◽

pp. 683-689 ◽

Cited By ~ 11

Author(s):

Bo Hu ◽

Minjian Liang ◽

Guoqiang Hong ◽

Zhaoxia Li ◽

Zhenyu Zhu ◽

...

Keyword(s):

Hepatitis B Virus ◽

Pichia Pastoris ◽

Hepatitis B ◽

S Gene ◽

B Virus

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The unforeseen during biotechnological therapy for moderate‐to‐severe psoriasis: How to manage pregnancy and breastfeeding, infections from Mycobacterium tuberculosis , hepatitis B virus, hepatitis C virus, and HIV , surgery, vaccinations, diagnosis of malignancy, and dose tapering

Dermatologic Therapy ◽

10.1111/dth.13411 ◽

2020 ◽

Vol 33 (3) ◽

Cited By ~ 1

Author(s):

Lidia Sacchelli ◽

Michela Magnano ◽

Camilla Loi ◽

Annalisa Patrizi ◽

Federico Bardazzi

Keyword(s):

Mycobacterium Tuberculosis ◽

Hepatitis C Virus ◽

Hepatitis C ◽

Hepatitis B Virus ◽

Hepatitis B ◽

Severe Psoriasis ◽

Virus Hepatitis ◽

B Virus

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High rate of hepatitis B virus S-integrated human extra spindle pole bodies like 1 fusion gene is detected in hepatitis B virus-related liver cancer patients: a Chinese case-control study

Journal of Hepatology ◽

10.1016/s0168-8278(20)32235-2 ◽

2020 ◽

Vol 73 ◽

pp. S903-S904

Author(s):

Bobin Hu ◽

Jianning Jiang ◽

Minghua Su ◽

Rongming Wang ◽

Weiwei Zang ◽

...

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Case Control Study ◽

Fusion Gene ◽

Spindle Pole ◽

High Rate ◽

Spindle Pole Bodies ◽

B Virus ◽

Chinese Case ◽

Control Study

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Synthesis and Assembly of Hepatitis B Virus-Like Particles in a Pichia pastoris Cell-Free System

Frontiers in Bioengineering and Biotechnology ◽

10.3389/fbioe.2020.00072 ◽

2020 ◽

Vol 8 ◽

Cited By ~ 1

Author(s):

Alex J. Spice ◽

Rochelle Aw ◽

Daniel G. Bracewell ◽

Karen M. Polizzi

Keyword(s):

Hepatitis B Virus ◽

Pichia Pastoris ◽

Hepatitis B ◽

Free System ◽

Virus Like Particles ◽

Cell Free System ◽

B Virus ◽

Pastoris Cell

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OPTIMAL CONDITIONS OF FERMENTATION OF THE PICHIA PASTORIS YEAST EXPRESSING THE RECOMBINANT PRES2-S PROTEIN (M-HBsAg) OF HEPATITIS B VIRUS (HBV)

CHEMISTRY AND CHEMICAL ENGINEERING ◽

10.51348/mpau8653 ◽

2020 ◽

Vol 2020 (3) ◽

pp. 56-59

Keyword(s):

Hepatitis B Virus ◽

Pichia Pastoris ◽

Hepatitis B ◽

Yeast Cells ◽

Enzyme Linked Immunosorbent Assay ◽

S Protein ◽

Wet Biomass ◽

B Virus ◽

Pichia Pastoris Yeast

The aim of this study is determination of optimal fermentation conditions of the Pichia pastoris yeast expressing the recombinant Pres2-S protein of hepatitis B virus (HBV). For this purpose we investigated the main factors influencing for the cultivation of recombinant strain of the yeast Pichia pastoris pPIC3,5-S-HBsAg - the composition of the nutrient medium, pH and dissolved oxygen (DO). As a result, by enzyme-linked immunosorbent assay (ELISA) the optimal concentration of methanol ≈1.0 %, which is the initiator and the only source of carbon for protein expression in the Mut+ phenotype Pichia pastoris yeast cells was determined. In the selected conditions at the end of the fermentation process, the wet biomass of yeast cells was 420 g/l.

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