Assessment of genetic diversity among wheat somaclonal variants lines using morphological traits and molecular markers

2011 ◽  
Vol 10 (66) ◽  
Author(s):  
Abouzied
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Morphological Traits ◽  
Somaclonal Variants

Genetic diversity and population structure of Algerian chickpea (Cicer arietinum) genotypes: use of agro-morphological traits and molecular markers linked or not linked to the gene or QTL of interest

2020 ◽  
Vol 71 (2) ◽  
pp. 155
Author(s):  
Djihad Bellemou ◽  
Teresa Millàn ◽  
Juan Gil ◽  
Aissa Abdelguerfi ◽  
Meriem Laouar
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Cicer Arietinum ◽  
Morphological Traits ◽  
Ascochyta Blight ◽  
Principal Component ◽  
Breeding Programs ◽  
Average Value ◽  
Conservation Of Genetic Resources ◽  
Resistant Genotypes

Assessment of genetic diversity among chickpea (Cicer arietinum L.) germplasm at the morphological and molecular levels is fundamental for chickpea breeding and conservation of genetic resources. Genetic variability of 46 chickpea genotypes including 42 Algerian genotypes and four control varieties was evaluated by using 15 agro-morphological traits. Eleven molecular markers including nine simple sequence repeats, one sequence characterised amplified region (SCY17) and one gene-specific (CaETR4) were used to characterise the 46 genotypes and eight references varieties added for disease resistance or susceptibility. Genotypes resistant to ascochyta blight were identified by the markers SCY17 and CaETR4 present together. High diversity was observed for all measured morphological traits between genotypes. Yield components, plant height, phenological traits and growth habit were the traits most involved in variation among genotypes and were partitioned into four groups by using principal component analysis. All molecular markers were polymorphic. In total, 91 alleles were obtained ranging from 2 to 21 per locus with average of 8.27 alleles per marker. Polymorphism information content ranged from 0.58 to 0.99 with an average value of 0.87. UPGMA clustering and Bayesian-based model structure analysis grouped genotypes into two clusters, but the distribution of the genotypes by cluster was not the same for the two analyses. According to the presence of markers indicating resistance to ascochyta blight (SCY17 and CaETR4), three resistant genotypes (FLIP 82-C92, ILC 6909, ILC 7241) were selected and should be tested in controlled conditions for confirmation. Considering the narrow diversity of cultivated chickpea, the Algerian genotypes can be considered as interesting for future breeding programs.

scholarly journals Characterization of genetic diversity of cactus species (Opuntia spp.) in Morocco by morphological traits and molecular markers

2017 ◽  
Vol 5 (2) ◽  
pp. 149-159 ◽  
Author(s):  
Y. El Kharrassi ◽  
M.A. Mazri ◽  
M.H. Sedra ◽  
A. Mabrouk ◽  
B . Nasser ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Morphological Traits ◽  
Polymorphic Information Content ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
Group Method ◽  
Rapd Primer ◽  
Opuntia Spp ◽  
Issr Primers

The genetic diversity within and among 124 accessions of Opuntia spp. collected from different regions of Morocco was assessed using morphological descriptors and molecular markers. Based on 10 morphological traits, the accessions were separated into 3 main clusters; each cluster was containing accessions from different regions and species. Polymerase chain reaction (PCR) was then performed on 22 accessions from different regions and species, with 10 inter-simple sequence repeat (ISSR) primers and one random amplified polymorphic DNA (RAPD) primer. ISSR primers produced 66 bands overall, 64 (96.9 %) of which were polymorphic while 6 bands were generated by the RAPD marker, all polymorphic. The polymorphic information content (PIC) values ranged from 0.62 to 0.97, with an average of 0.82. The dendrogram of genetic differences generated using the unweighted pair-group method using arithmetic averages (UPGMA) method showed 7 different clusters at a similarity of 0.76, which was confirmed by the principal component analysis (PCA). The main conclusion of our work is the high genetic similarity between Opuntia ficus indica and Opuntia megacantha species in Morocco. Our results will be useful for plant breeding and genetic resource conservation programs.

scholarly journals Genetic diversity in maize dent landraces assessed by morphological and molecular markers

Genetika ◽  
2013 ◽  
Vol 45 (3) ◽  
pp. 811-824 ◽  
Author(s):  
Danijela Ristic ◽  
Vojka Babic ◽  
Violeta Andjelkovic ◽  
Jelena Vancetovic ◽  
Snezana Mladenovic-Drinic ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Molecular Markers ◽  
Genetic Distance ◽  
Morphological Traits ◽  
Mean Value ◽  
Ecological Data ◽  
Phenotypic Analysis ◽  
High Level ◽  
Maize Research Institute

Maize Research Institute ?Zemun Polje? genebank maintains a collection of landraces grouped into 18 agro-ecological collected from ex-Yugoslavia territories. The application and comparison of different marker systems are important for the characterization and use of maize landraces in breeding program, as potential sources of desirable traits. In this study, 15 morphological traits, 7 RAPD primers and 10 SSR primer pairs were applied to i) to determine genetic distance between 21 maize dent landraces and ii) compare results obtained on morphological and molecular markers. Phenotypic analysis showed high level of heterogeneity between landraces. Higher level of genetic diversity was obtained with SSR than with RAPD. Genetic distance mean value for RAPD data was 0.35 i.e. for SSR 0.48. Based on the morphological traits and molecular markers, unweighted pairgroup method (UPGMA) analysis was applied for cluster analysis, using statistical NTSYSpc program package. Cluster analysis of morphological and molecular markers distances did not show the same population grouping. Better agreement with agro-ecological data was obtained with RAPD markers. Correlations between dissimilarity matrices for different types of markers were low. Data obtained in this work could be useful for further study of a larger number of landraces, and conservation of genetic resources and their genetic diversity.

Genetic diversity of Iranian Aegilops tauschii Coss. using microsatellite molecular markers and morphological traits

2009 ◽  
Vol 37 (7) ◽  
pp. 3413-3420 ◽  
Author(s):  
Zahra Tahernezhad ◽  
Mohammad Javad Zamani ◽  
Mahmood Solouki ◽  
Mehdi Zahravi ◽  
Abbas Ali Imamjomeh ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Morphological Traits ◽  
Aegilops Tauschii

scholarly journals Genetic diversity of Albanian pea (Pisum sativum L.) landraces assessed by morphological traits and molecular markers

2014 ◽  
Vol 50 (No. 2) ◽  
pp. 177-184 ◽  
Author(s):  
B. Gixhari ◽  
M. Pavelková ◽  
H. Ismaili ◽  
H. Vrapi ◽  
A. Jaupi ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Molecular Markers ◽  
Morphological Traits ◽  
Principal Component ◽  
Morphological Characters ◽  
Insertion Polymorphism ◽  
Growing Seasons ◽  
And Cluster Analysis

In order to investigate the genetic diversity present in the pea germplasm stored in the Albanian genebank, we analyzed 28 local pea genotypes of Albanian origins for 23 quantitative morphological traits, as well as 14 retrotransposon-based insertion polymorphism (RBIP) molecular markers. The study of morphological characters carried out during three growing seasons (2010, 2011 and 2012) had the objective of characterization of traits useful in breeding programs. RBIP marker analysis revealed the genetic similarity in range from 0.06 to 0.45. ANOVA, principal component analysis (PCA) and cluster analysis was used to visualize the association among different traits. Most of the quantitative morphological traits showed significant differences. PCA and cluster analysis (Ward’s method) carried out for morphological traits divided the local pea genotypes into three clusters. Finally, the study identified the agronomicaly important traits which will facilitate the maintenance and agronomic evaluation of the collections.

scholarly journals 561 Using AFLPs to Examine Genetic Diversity in Umbrella Fern

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 543A-543
Author(s):  
F.J. Keiper ◽  
R. McConchie
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Molecular Markers ◽  
Dna Markers ◽  
Morphological Traits ◽  
Natural Populations ◽  
Cost Effective ◽  
Molecular Techniques ◽  
Breeding Systems ◽  
Detailed Knowledge

Umbrella fern [Sticherus flabellatus (R. Br.) St John] is a successful Australian native foliage product. Currently, all umbrella fern sold on the market is bush-harvested. To meet the growing demand for this product on local and international markets, a commercially viable method for its production must be developed, with effective management of the germplasm resource in terms of conservation and exploitation. To manage this resource, breeders require a detailed knowledge of the amount and distribution of genetic variability within the species. Traditionally, plant breeders focus on a combination of agronomic and morphological traits (phenotype) to measure genetic diversity. In umbrella fern there are a limited number of morphological traits, and these are influenced by environmental factors and therefore do not reflect true genetic diversity. To overcome these problems, molecular techniques such as PCR-based DNA markers are used to complement traditional strategies for genotype assessment. DNA markers have the advantages of being independent of environmental effects, as well as being fast, cost-effective, reproducible, and largely accessible to the nonmolecular geneticist. Amplified fragment length polymorphisms (AFLPs) fulfil many of the desirable features of molecular markers, as well as requiring little knowledge of the genome to be investigated. AFLPs have been used widely in the analysis of breeding systems, ecogeographical variation, and genetic variation within and between natural populations. To date there are no published accounts of DNA molecular marker research on umbrella fern. A DNA extraction protocol has been developed for this species, and AFLP markers have been used to analyse genetic diversity within and between natural populations sampled in the Sydney Basin. A large number of polymorphic loci were revealed using 11 primer combinations. The genetic variation detected was partitioned between rather than within populations, suggesting that the mating system in Sticherus is primarily inbreeding. Data will be presented illustrating AFLPs as useful molecular markers for assessing genetic diversity within and between populations of umbrella fern and providing insight on the breeding system used by the species.

scholarly journals Genetic Diversity in <i>Jatropha platyphylla</i> Accessions Based on Morphological Traits and Inter-Simple Sequence Repeats Molecular Markers

2021 ◽  
Vol 12 (11) ◽  
pp. 1658-1672
Author(s):  
Edith Salazar-Villa ◽  
Lilia Alcaraz-Meléndez ◽  
Josefina León-Félix ◽  
José Basilio Heredia ◽  
Federico Soto-Landeros ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Simple Sequence Repeats ◽  
Morphological Traits ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence
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