scholarly journals Virulence factors and antibiotic susceptibility among verotoxic non O157: H7 Escherichia coli isolates obtained from water and wastewater samples in Cape Town, South Africa.

2011 ◽  
Vol 10 (64) ◽  
pp. 14160-14168 ◽  
Author(s):  
James Doughari Hamuel ◽  
Alois Ndakidemi Patrick ◽  
Susan Human Izanne ◽  
Benade Spinney
Keyword(s):  
Escherichia Coli ◽  
South Africa ◽  
Virulence Factors ◽  
Antibiotic Susceptibility ◽  
Cape Town ◽  
Water And Wastewater ◽  
Wastewater Samples

scholarly journals ANTIBIOTIC SUSCEPTIBILITY OF MICROORGANISMS ISOLATED FROM WASTEWATER AT AL-HIKMAH UNIVERSITY FEMALE HOSTELS, ILORIN, NIGEERIA

2020 ◽  
Vol 7 ◽  
pp. 58
Author(s):  
Ismail Babatunde Onajobi ◽  
Olusolape Afolake Favour Ilusanya, ◽  
Omowunmi Abosede Banjo ◽  
Abdul-Azeez Olayiwola Sirajudeen
Keyword(s):  
Escherichia Coli ◽  
Electrical Conductivity ◽  
Antibiotic Susceptibility ◽  
Proteus Vulgaris ◽  
Negative Effects ◽  
Antimicrobial Sensitivity ◽  
A Value ◽  
Standard Procedures ◽  
Sensitivity Tests ◽  
Wastewater Samples

Physicochemical properties and antibiotic susceptibility of microorganisms isolated from wastewater samples collected from Al-Hikmah University female hostels were assessed. Samples obtained from different female hostels were examined physiochemically and microbiologically using standard procedures. Phytochemical result revealed the low temperature value of 28°C from Oladimeji and Commaissie hostels, while Deremi and Owaish had a value of 29.5°C. Dissolved oxygen of  0.1 ml/L was recorded from all the samples collected. Electrical conductivity ranged from 658 to 997µs, while chocking odour was recorded in all the four samples collected. In terms of pH, Oladimeji hostel had a pH of 10.5, Deremi had a pH of 10.6, while Owaish and Commaissie had a pH of 10.4. The wastewater samples from the four hostels were highly contaminated. Oladimeji hostel showed a mean bacterial 5count of 25.4×10cfu/ml, Deremi hostel, Owaish hostel and Coomassie hostels, showed a mean 4 4 4bacterial count of 5.7×10cfu/ml,16.8×10cfu/ml and 3.5×10cfu/ml respectively. Percentage occurrence of bacteria isolates were Salmonella sp. (12.5%), Klebsiella pneumoniae (12.5%), Escherichia coli (25%), Staphylococcus aureus (12.5%), Actinomyces (12.5%), Shigella sp. (6.25%), Pseudomonas aeruginosa (12.5%) and Proteus vulgaris (6.25%). Antimicrobial sensitivity tests revealed that all the organisms were susceptible to gentamycin except Actinomyces. High microbial load in wastewater samples could have negative effects on human health. This study, further confirms the need to treat wastewater rather than directly discharging it to the environment.

scholarly journals Characterization of Shiga Toxin Gene (stx)-Positive and Intimin Gene (eae)-Positive Escherichia coli Isolates from Wastewater of Slaughterhouses in France

2006 ◽  
Vol 72 (5) ◽  
pp. 3245-3251 ◽  
Author(s):  
Estelle Loukiadis ◽  
Monique K�rour�dan ◽  
Lothar Beutin ◽  
Eric Oswald ◽  
Hubert Brug�re
Keyword(s):  
Escherichia Coli ◽  
Virulence Factors ◽  
Wastewater Treatment Plants ◽  
Immunomagnetic Separation ◽  
Toxin Gene ◽  
E Coli ◽  
Treated Effluent ◽  
Colony Counting ◽  
Wastewater Samples

ABSTRACT Wastewater samples from 12 slaughterhouses located in different regions in France were tested for the presence of stx-positive and eae-positive Escherichia coli isolates, and characteristics of the isolates obtained were determined. A total of 224 wastewater samples were collected in wastewater treatment plants at different stages of wastewater processing. Altogether, 5,001 E. coli isolates were obtained by colony counting and screened for the presence of stx and eae genes by multiplex PCR. stx-positive and eae-positive E. coli isolates were detected in 25% of the samples collected; they were found in 13% and 3% of the samples obtained from treated effluent and sludge, respectively, suggesting that they could be spread into the environment. Screening of the samples collected by immunomagnetic separation allowed us to isolate 31 additional E. coli serogroup O157 isolates. Four of these isolates harbored stx and eae genes. All stx-positive and eae-positive E. coli isolates were analyzed for eae and stx genetic variants, as well as for additional virulence factors and serotypes. Our results suggest that the majority of the stx- and eae-positive E. coli isolates from wastewater have low virulence for humans. However, the diversity of the enterohemorrhagic E. coli-associated virulence factors in the strains indicates that the environment may play an important role in the emergence of new pathogenic enterohemorrhagic E. coli strains.

scholarly journals A DNA pooling based system to detect Escherichia coli virulence factors in fecal and wastewater samples

2012 ◽  
Vol 43 (4) ◽  
pp. 1319-1326 ◽  
Author(s):  
J Luz María Chacón ◽  
C Lizeth Taylor ◽  
A Carmen Valiente ◽  
P Irene Alvarado ◽  
B Ximena Cortés
Keyword(s):  
Escherichia Coli ◽  
Virulence Factors ◽  
Dna Pooling ◽  
Wastewater Samples
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