scholarly journals A flow cytometric assay for simultaneously measuring the proliferation and cytotoxicity of cytokine induced killer cells in combination with carboxyfluorescein succinimidyl ester (CFSE) labeling

2011 ◽  
Vol 10 (65) ◽  
pp. 14598-14607 ◽  
Author(s):  
Duan Xiu Mei ◽  
Huang Jing ◽  
Ma Hong Xi ◽  
Wang Xiao Qi ◽  
Che Yuan Yuan ◽  
...  
Keyword(s):  
Flow Cytometric Assay ◽  
Killer Cells ◽  
Flow Cytometric ◽  
Succinimidyl Ester ◽  
Carboxyfluorescein Succinimidyl Ester

scholarly journals A simple and sensitive flow cytometric assay for the determination of the cytotoxic activity of human natural killer cells

1990 ◽  
Vol 135 (1-2) ◽  
pp. 81-89 ◽  
Author(s):  
Katarina Radošević ◽  
Henk S.P. Garritsen ◽  
Marja Van Graft ◽  
Bart G. De Grooth ◽  
Jan Greve
Keyword(s):  
Natural Killer Cells ◽  
Cytotoxic Activity ◽  
Natural Killer ◽  
Flow Cytometric Assay ◽  
Killer Cells ◽  
Flow Cytometric ◽  
Human Natural Killer Cells

scholarly journals Nuclear oncoprotein expression as a function of lineage, differentiation stage, and proliferative status of normal human hematopoietic cells

Blood ◽  
1989 ◽  
Vol 74 (5) ◽  
pp. 1517-1524 ◽  
Author(s):  
MB Kastan ◽  
KD Stone ◽  
CI Civin
Keyword(s):  
Progenitor Cells ◽  
Control Cell ◽  
Hematopoietic Cells ◽  
Maturation Stage ◽  
Flow Cytometric Assay ◽  
Lineage Differentiation ◽  
Flow Cytometric ◽  
A Cell ◽  
Normal Human ◽  
Differentiation Stage

Abstract Relative levels of the nuclear oncoproteins c-myb, c-myc, and c-fos were determined in selected subpopulations of normal human bone marrow (BM) cells using a flow cytometric assay which simultaneously detects a cell-surface antigen (as a marker of lineage and stage of maturation) and levels of an intracellular protein. At least two monoclonal antibodies directed against each oncoprotein and specific peptide inhibition controls were used for these determinations. Hematopoietic progenitor cells (CD34+) express the highest levels of c-myb and c-myc, whereas c-fos levels in CD34+ progenitor cells are similar to c-fos levels in mature monocytes and granulocytes. Granulocytes are the only hematopoietic cells examined which do not express detectable levels of c-myb and c-myc. The levels of these oncoproteins in these normal, unstimulated BM cell populations were more closely linked to lineage and maturation stage than to the proliferative status of the given population, as determined by either DNA staining or expression of the cell-cycle specific nuclear protein, Ki67. This flow cytometric assay helps in interpreting the significance of oncoprotein levels in leukemia cells by allowing direct comparisons of a leukemia with the phenotypically similar “normal counterpart control” cell population in normal BM.

A Flow Cytometric Assay for Lysosomal Glucocerebrosidase

1997 ◽  
Vol 247 (2) ◽  
pp. 268-271 ◽  
Author(s):  
Helmuth H.G. van Es ◽  
Marlon Veldwijk ◽  
Menzo Havenga ◽  
Dinko Valerio
Keyword(s):  
Flow Cytometric Assay ◽  
Flow Cytometric

Detection of Circulating Endothelial Cells: CD146-Based Magnetic Separation Enrichment or Flow Cytometric Assay?

2007 ◽  
Vol 25 (5) ◽  
pp. e1-e2 ◽  
Author(s):  
Françoise Dignat-George ◽  
Florence Sabatier ◽  
Andrew Blann ◽  
Alexander Woywodt
Keyword(s):  
Endothelial Cells ◽  
Magnetic Separation ◽  
Circulating Endothelial Cells ◽  
Flow Cytometric Assay ◽  
Flow Cytometric
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