scholarly journals In vitro protective effect of Pandanus ordoratissimus extract on ultraviolet B (UVB)-induced DNA damage

2011 ◽  
Vol 10 (45) ◽  
pp. 9165-9169 ◽  
Author(s):  
Kaewklom Siriporn ◽  
Vejaratpimol Renu
Keyword(s):  
Dna Damage ◽  
Protective Effect ◽  
Ultraviolet B

Protective effect of argan oil on DNA damage in vivo and in vitro

Biomarkers ◽  
2021 ◽  
pp. 1-9
Author(s):  
Habiba Bouchab ◽  
Abbas Ishaq ◽  
Riad EL Kebbaj ◽  
Boubker Nasser ◽  
Gabriele Saretzki
Keyword(s):  
Dna Damage ◽  
Protective Effect ◽  
Argan Oil

scholarly journals hSSB2 (NABP1) is required for the recruitment of RPA during the cellular response to DNA UV damage

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Didier Boucher ◽  
Ruvini Kariawasam ◽  
Joshua Burgess ◽  
Adrian Gimenez ◽  
Tristan E. Ocampo ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cellular Response ◽  
Excision Repair ◽  
Protein A ◽  
Ultraviolet B ◽  
Repair System ◽  
Uvb Radiation ◽  
Exogenous Dna

AbstractMaintenance of genomic stability is critical to prevent diseases such as cancer. As such, eukaryotic cells have multiple pathways to efficiently detect, signal and repair DNA damage. One common form of exogenous DNA damage comes from ultraviolet B (UVB) radiation. UVB generates cyclobutane pyrimidine dimers (CPD) that must be rapidly detected and repaired to maintain the genetic code. The nucleotide excision repair (NER) pathway is the main repair system for this type of DNA damage. Here, we determined the role of the human Single-Stranded DNA Binding protein 2, hSSB2, in the response to UVB exposure. We demonstrate that hSSB2 levels increase in vitro and in vivo after UVB irradiation and that hSSB2 rapidly binds to chromatin. Depletion of hSSB2 results in significantly decreased Replication Protein A (RPA32) phosphorylation and impaired RPA32 localisation to the site of UV-induced DNA damage. Delayed recruitment of NER protein Xeroderma Pigmentosum group C (XPC) was also observed, leading to increased cellular sensitivity to UVB. Finally, hSSB2 was shown to have affinity for single-strand DNA containing a single CPD and for duplex DNA with a two-base mismatch mimicking a CPD moiety. Altogether our data demonstrate that hSSB2 is involved in the cellular response to UV exposure.

Polyhydroxylated 4-Thiaflavans as Multipotent Antioxidants: Protective Effect on Oxidative DNA Damage in vitro.

ChemInform ◽  
2006 ◽  
Vol 37 (29) ◽  
Author(s):  
Maura Lodovici ◽  
Stefano Menichetti ◽  
Caterina Viglianisi ◽  
Silvia Caldini ◽  
Elisa Giuliani
Keyword(s):  
Dna Damage ◽  
Protective Effect ◽  
Oxidative Dna Damage

In vitro study of the protective effect of manganese against vanadium-mediated nuclear and mitochondrial DNA damage

2020 ◽  
Vol 135 ◽  
pp. 110900 ◽  
Author(s):  
Lorenzo Rivas-García ◽  
José L. Quiles ◽  
Alfonso Varela-López ◽  
Miguel Arredondo ◽  
Paulina Lopez ◽  
...  
Keyword(s):  
Dna Damage ◽  
Mitochondrial Dna ◽  
Protective Effect ◽  
In Vitro Study ◽  
Vitro Study ◽  
Mitochondrial Dna Damage

scholarly journals Echinacoside Alleviates UVB Irradiation-Mediated Skin Damage via Inhibition of Oxidative Stress, DNA Damage, and Apoptosis

2017 ◽  
Vol 2017 ◽  
pp. 1-15 ◽  
Author(s):  
Di Zhang ◽  
Chengtao Lu ◽  
Zhe Yu ◽  
Xiayin Wang ◽  
Li Yan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Histopathological Examination ◽  
Ultraviolet B ◽  
Ros Generation ◽  
Antioxidant Enzyme Activities ◽  
Skin Damage ◽  
Uvb Irradiation ◽  
Uvb Exposure

Ultraviolet B (UVB) irradiation has been known to cause skin damage, which is associated with oxidative stress, DNA damage, and apoptosis. Echinacoside is a phenylethanoid glycoside isolated from Herba Cistanches, which exhibits strong antioxidant activity. In this study, we evaluate the photoprotective effect of echinacoside on UVB-induced skin damage and explore the potential molecular mechanism. BALB/c mice and HaCaT cells were treated with echinacoside before UVB exposure. Histopathological examination was used to evaluate the skin damage. Cell viability, lactate dehydrogenase (LDH) levels, antioxidant enzyme activities, reactive oxygen species (ROS) generation, DNA damage, and apoptosis were measured as well. Western blot was used to measure the expression of related proteins. The results revealed that pretreatment of echinacoside ameliorated the skin injury; attenuated oxidative stress, DNA damage, and apoptosis caused by UVB exposure; and normalized the protein levels of ATR, p53, PIAS3, hnRNP K, PARP, and XPA. To summarize, echinacoside is beneficial in the prevention of UVB-induced DNA damage and apoptosis of the skin in vivo and in vitro.

Polyhydroxylated 4-thiaflavans as multipotent antioxidants: Protective effect on oxidative DNA damage in vitro

2006 ◽  
Vol 16 (7) ◽  
pp. 1957-1960 ◽  
Author(s):  
Maura Lodovici ◽  
Stefano Menichetti ◽  
Caterina Viglianisi ◽  
Silvia Caldini ◽  
Elisa Giuliani
Keyword(s):  
Dna Damage ◽  
Protective Effect ◽  
Oxidative Dna Damage

scholarly journals Niosome-carvedilol protects DNA damage of supraphysiologic concentrations of insulin using comet assay: An in vitro study

2021 ◽  
pp. 096032712110361
Author(s):  
Marzieh Farahani-Zangaraki ◽  
Azade Taheri ◽  
Mahmoud Etebari
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Dna Damage ◽  
Comet Assay ◽  
Protective Effect ◽  
Reactive Oxygen ◽  
Diabetic Patients ◽  
Oxygen Species ◽  
Type 2 Diabetic Patients

Introduction: Hyperinsulinemia occurs in type 2 diabetic patients with insulin resistance. This increase in insulin levels in the blood increases reactive oxygen species production and oxidative stress, resulting in DNA damage. Carvedilol (CRV) is a non-selective beta-blocker, and research has shown that this compound and its metabolites have anti-oxidative properties. Carvedilol can, directly and indirectly, reduce reactive oxygen species (ROS) and has a protective effect on DNA damage from oxidative stress. Given the insolubility of CRV in water, finding new methods to increase its solubility can be an essential step in research. This study aimed to determine whether carvedilol could have a protective effect on insulin-induced genomic damage. Methods: We treated cells with insulin alone, amorphous-CRV alone, and amorphous-CRV and niosomal-CRV with insulin and DNA damage were investigated using the comet method to achieve this goal. Results: Our results showed that insulin in the studied concentration has a significant genotoxic effect and non-cytotoxic at higher concentrations. CRV, both in amorphous and niosome form, reduced insulin-induced DNA damage by reducing ROS production. The comet assay results demonstrate that treating HUVEC cells in pretreatment condition with amorphous-CRV and niosome-CRV significantly reduces DNA damage of insulin.

scholarly journals Saireito Improves Lymphatic Function and Prevents UVB-Induced Acute Inflammation and Photodamage in HR-1 Hairless Mice

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Manami Oyama ◽  
Kenta Murata ◽  
Misaki Ogata ◽  
Nina Fujita ◽  
Ryuji Takahashi
Keyword(s):  
Protective Effect ◽  
Proinflammatory Cytokines ◽  
Inflammatory Responses ◽  
Early Stage ◽  
Ultraviolet B ◽  
High Dose ◽  
Hairless Mice ◽  
Edema Formation ◽  
Lymphatic Function

A single high-dose ultraviolet B (UVB) exposure on the skin induces acute inflammatory responses, such as an increase in proinflammatory cytokines (e.g., IL-6 and IL-1β), hyperpermeability and dilation of blood and lymphatic vessels, and infiltration of inflammatory cells. These responses result in different cutaneous disorders characterized by erythema, epidermal hyperplasia, edema formation, and extracellular matrix degradation. Saireito extract (SRT), a traditional Chinese medicine, has been used to treat various inflammatory diseases in Japan, and SRT and its major active components (e.g., saikosaponins and baicalin) were reported to downregulate proinflammatory cytokines. Moreover, SRT has a protective effect against UV irradiation in vitro. Based on these findings, we aimed to investigate the effect of SRT on UVB-induced photodamage and structural change in the vasculature. We pretreated male HR-1 hairless mice with SRT (625 or 1250 mg/kg) for 3 weeks before a single UVB (250 mJ/cm2) irradiation. SRT treatment attenuated UVB-induced increases in erythema, transepidermal water loss, and edema formation at 72 h after irradiation. SRT treatment also suppressed UVB-induced inflammatory cell infiltration and collagen degradation. Furthermore, at 24 h after irradiation, SRT treatment inhibited UVB-induced upregulation of proinflammatory cytokines and reduction in lymphatic vessel density associated with upregulation of VEGF-C expression. These results suggest that SRT could attenuate UVB-induced photodamage. This protective effect of SRT involves suppression of upregulation of proinflammatory cytokines and improvement of lymphatic function in the early stage of inflammation.

scholarly journals DNA damage in homocystinuria: 8-oxo-7,8-dihydro-2 -deoxyguanosine levels in cystathionine-²-synthase deficient patients and the in vitro protective effect of N-acetyl-L-cysteine

2018 ◽  
Vol 38 (1) ◽  
pp. 50-57
Author(s):  
Camila Simioni Vanzin ◽  
Caroline Paula Mescka ◽  
Bruna Donida ◽  
Desirèe Padilha Marchetti ◽  
Carlos Eduardo Jacques ◽  
...  
Keyword(s):  
Dna Damage ◽  
Protective Effect
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