scholarly journals Isolation and characterization of Jatropha oil-degradation by Enterococcus faecalis and Burkholderia cenocepacia W-1 under anaerobic condition

2011 ◽  
Vol 10 (63) ◽  
pp. 13841-13851 ◽  
Author(s):  
Boontawan Apichat ◽  
Boontawan Pailin
Keyword(s):  
Enterococcus Faecalis ◽  
Anaerobic Condition ◽  
Burkholderia Cenocepacia ◽  
Oil Degradation ◽  
Jatropha Oil ◽  
Isolation And Characterization

Isolation and Characterization of Enterocin SE-K4 Produced by Thermophilic Enterococci, Enterococcus faecalis K-4

2001 ◽  
Vol 65 (2) ◽  
pp. 247-253 ◽  
Author(s):  
Tomoko EGUCHI ◽  
Kazuyo KAMINAKA ◽  
Jun SHIMA ◽  
Shinichi KAWAMOTO ◽  
Katsumi MORI ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Isolation And Characterization

Isolation and Characterization of Petroleum Hydrocarbon Degrading Bacteria from the Bohai Sea, China

2014 ◽  
Vol 955-959 ◽  
pp. 728-731
Author(s):  
Ping Guo ◽  
Jian Guo Lin ◽  
Bin Xia Cao ◽  
Na Ta
Keyword(s):  
Petroleum Hydrocarbon ◽  
Bohai Sea ◽  
Diesel Oil ◽  
Contaminated Site ◽  
Oil Degradation ◽  
Isolation And Characterization ◽  
Degrading Bacteria ◽  
Luxuriant Growth ◽  
Diesel Oil Degradation

Fourteen petroleum hydrocarbon degrading bacteria strains were isolated from oil-contaminated site. Isolated strains were able use diesel oil as sole carbon and energy source. Bacterial strain HD1 was selected due to the luxuriant growth on oil agar. The oil degradation rate of strain HD1 was analyzed using UV-spectrometry-based methods. The result showed that the rate of diesel oil degradation of 75% was observed after 14days of cultivation.

Isolation and characterization of genetic expression and secretion signals from Enterococcus faecalis through the use of broad-host-range α-amylase probe vectors

Gene ◽  
1992 ◽  
Vol 118 (1) ◽  
pp. 21-30 ◽  
Author(s):  
Pascal Hols ◽  
Alain Baulard ◽  
Dominique Garmyn ◽  
Brigitte Delplace ◽  
Stéphane Hogan ◽  
...  
Keyword(s):  
Host Range ◽  
Enterococcus Faecalis ◽  
Broad Host Range ◽  
Genetic Expression ◽  
Isolation And Characterization

Isolation and Characterization of a Novel Bacteriophage φ4D Lytic Against Enterococcus faecalis Strains

2012 ◽  
Vol 65 (3) ◽  
pp. 284-289 ◽  
Author(s):  
Sylwia Parasion ◽  
Magdalena Kwiatek ◽  
Lidia Mizak ◽  
Romuald Gryko ◽  
Michał Bartoszcze ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Burkholderia cenocepacia Mutants Deficient in Pyochelin Production: Pyochelin Biosynthesis Is Sensitive to Sulfur Availability

2004 ◽  
Vol 186 (2) ◽  
pp. 270-277 ◽  
Author(s):  
Kate L. Farmer ◽  
Mark S. Thomas
Keyword(s):  
Wild Type Strain ◽  
Opportunistic Pathogen ◽  
Burkholderia Cenocepacia ◽  
Wild Type ◽  
Iron Starvation ◽  
Gene Encoding ◽  
Isolation And Characterization ◽  
Putative Operon ◽  
Green Fluorescent

ABSTRACT The opportunistic pathogen Burkholderia cenocepacia produces the yellow-green fluorescent siderophore, pyochelin. To isolate mutants which do not produce this siderophore, we mutagenized B. cenocepacia with the transposon mini-Tn5Tp. Two nonfluorescent mutants were identified which were unable to produce pyochelin. In both mutants, the transposon had integrated into a gene encoding an orthologue of CysW, a component of the sulfate/thiosulfate transporter. The cysW gene was located within a putative operon encoding other components of the transporter and a polypeptide exhibiting high homology to the LysR-type regulators CysB and Cbl. Sulfate uptake assays confirmed that both mutants were defective in sulfate transport. Growth in the presence of cysteine, but not methionine, restored the ability of the mutants to produce pyochelin, suggesting that the failure to produce the siderophore was the result of a depleted intracellular pool of cysteine, a biosynthetic precursor of pyochelin. Consistent with this, the wild-type strain did not produce pyochelin when grown in the presence of lower concentrations of sulfate that still supported efficient growth. We also showed that whereas methionine and certain organosulfonates can serve as sole sulfur sources for this bacterium, they do not facilitate pyochelin biosynthesis. These observations suggest that, under conditions of sulfur depletion, cysteine cannot be spared for production of pyochelin even under iron starvation conditions.

scholarly journals Isolation and characterization of an Enterococcus faecalis bacteriophage

2015 ◽  
Vol 51 (3) ◽  
pp. 194-198
Author(s):  
Hee-Young Kang ◽  
Shukho Kim ◽  
Jungmin Kim
Keyword(s):  
Enterococcus Faecalis ◽  
Isolation And Characterization

Isolation and characterization of enterocin EJ97, a bacteriocin produced by Enterococcus faecalis EJ97

1998 ◽  
Vol 171 (1) ◽  
pp. 59-65 ◽  
Author(s):  
A. Gálvez ◽  
E. Valdivia ◽  
Hikmate Abriouel ◽  
Emilio Camafeita ◽  
Enrique Mendez ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Enterococcus faecalis-Infecting Bacteriophages From Different Cheese Types

2021 ◽  
Vol 11 ◽  
Author(s):  
Beatriz del Rio ◽  
Esther Sánchez-Llana ◽  
Noelia Martínez ◽  
María Fernández ◽  
Victor Ladero ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Fermented Foods ◽  
Isolation And Characterization ◽  
Different Types ◽  
Clinical Environments ◽  
Genomic Characterization ◽  
Diverse Origin ◽  
Hospital Acquired ◽  
Silver Bullet

Enterococci are a diverse group of Gram-positive, lactic acid bacteria (LAB). They are found in many environments, including fermented foods, in which they could constitute a health threat since they produce biogenic amines, which consumption can lead to intoxication. Moreover, enterococci has also emerged as an important hospital-acquired pathogens via its acquisition of antimicrobial resistance. Bacteriophages possess features that make them optimal biotechnological weapons for controlling bacterial growth in disease and food spoilage contexts. However, no silver bullet bacteriophage exists that can eliminate all the undesirable bacteria in a complex environment. Rather, a combination of phages with different host ranges would be required which implies the need for large collections of diverse phages. This work reports the isolation of several Enterococcus faecalis-infecting bacteriophages from different types of cheese, along with the range of E. faecalis strains of diverse origin (from dairy to clinical environments) they are able to infect. The isolated phages showed a large diversity regarding the number and origin of strains they infect. Some of these phages were subjected to morphological and genomic characterization which confirmed their diversity and showed they belong to different families and genera. The present findings increase the potential arsenal for the bacteriophage-based biocontrol of harmful E. faecalis populations.

Isolation and Characterization of Bile Salts-Sensitive Mutants of Enterococcus faecalis

2002 ◽  
Vol 45 (6) ◽  
pp. 0434-0439 ◽  
Author(s):  
Yoann Le Breton ◽  
Alain Mazé ◽  
Axel Hartke ◽  
Sylviane Lemarinier ◽  
Yanick Auffray ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Bile Salts ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Enterocin W, a Novel Two-Peptide Lantibiotic Produced by Enterococcus faecalis NKR-4-1

2011 ◽  
Vol 78 (3) ◽  
pp. 900-903 ◽  
Author(s):  
Naruhiko Sawa ◽  
Pongtep Wilaipun ◽  
Seisuke Kinoshita ◽  
Takeshi Zendo ◽  
Vichien Leelawatcharamas ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Enterococcus Faecalis ◽  
Inhibitory Activity ◽  
Content Type ◽  
Isolation And Characterization

ABSTRACTEnterococcus faecalisNKR-4-1 isolated from pla-ra produces a novel two-peptide lantibiotic, termed enterocin W, comprising Wα and Wβ. The structure of enterocin W exhibited similarity with that of plantaricin W. The two peptides acted synergistically, and their order of binding to the cell membrane was important for their inhibitory activity.

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