scholarly journals Preliminary investigations of Agrobacterium-mediated transformation in indica rice MR219 embryogenic callus using gusA gene

2011 ◽  
Vol 10 (40) ◽  
pp. 7805-7813 ◽  
Author(s):  
Ab Rahman Zuraida ◽  
Ahmad Seman Zulkifli ◽  
Basirun Naziah ◽  
Lizah Julkifle Advina ◽  
Zainal Zamri ◽  
...  
Keyword(s):  
Embryogenic Callus ◽  
Indica Rice ◽  
Agrobacterium Mediated Transformation ◽  
Gusa Gene

scholarly journals Establishment of an Efficient Regeneration System Amenable to Agrobacterium Mediated Transformation of Two Elite Indica Rice Varieties of North East India

2015 ◽  
Vol 3 (4) ◽  
pp. 680-686 ◽  
Author(s):  
Mohitosh Dey ◽  
Sanjib Kumar Panda ◽  
Lingaraj Sahoo
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Indica Rice ◽  
Northeast India ◽  
Regeneration System ◽  
Embryogenic Calli ◽  
Rice Varieties ◽  
Agrobacterium Mediated Transformation ◽  
North East

An efficient plant regeneration system from embryogenic callus of two elite indica rice (Oryza sativa spp. indica) varieties of Northeast India, Ketokijoha and Monoharsali is established. The effect of auxin, 2,4-dicholorophenoxy acetic acid (2,4-D) on callus induction was optimized. Friable, nodular and creamish-white embryogenic calli were induced from mature seeds on NB medium supplemented with 2.5 mg/l 2,4-D. Plants were regenerated from 40-50 days old embryogenic callus on NB medium containing 0.5 mg/l BAP (6-benzylaminopurine) and 0.25 mg/l ABA (abscisic acid). Regenerated plants with multiple tillers were rooted on half strength MS medium and rooted plants were acclimatized with 94% survival rate. Higher frequency of callus induction as well as plant regeneration was recorded in Ketokijoha as compared to Monoharsali. The calli of both the varieties were found amenable to Agrobacterium-mediated transformation as evident from strong GUS (β-glucuronidase) expression. The results may find wide application for genetic improvement for valuable traits these elite indica rice varieties of Northeast India. Int J Appl Sci Biotechnol, Vol 3(4): 680-686

scholarly journals High frequency plant regeneration from embryogenic callus of a popular indica rice (Oryza sativa L.)

2009 ◽  
Vol 15 (4) ◽  
pp. 371-375 ◽  
Author(s):  
Alagarsamy Karthikeyan ◽  
Shunmugiah Thevar Karutha Pandian ◽  
Manikandan Ramesh
Keyword(s):  
Oryza Sativa ◽  
Plant Regeneration ◽  
High Frequency ◽  
Embryogenic Callus ◽  
Oryza Sativa L ◽  
Indica Rice

scholarly journals Agrobacterium-mediated transformation of indica rice under Acetosyringone-free conditions

2007 ◽  
Vol 24 (5) ◽  
pp. 507-511 ◽  
Author(s):  
Mangu Venkata Ramana Rao ◽  
Gundimeda Jwala Narasimha Rao
Keyword(s):  
Indica Rice ◽  
Agrobacterium Mediated Transformation

scholarly journals Enhancement of Regeneration Efficiency through Callus Induction Media Using 2,4-Dicholorophenoxyacetic acid in Indica Rice (Oryza sativa L. var. Ciherang)

2017 ◽  
Vol 18 (2) ◽  
pp. 91
Author(s):  
Kunti Anis Azizah ◽  
Didik Pudji Restanto ◽  
Bambang Sugiharto
Keyword(s):  
Oryza Sativa ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
Rice Variety ◽  
Rice Cultivar ◽  
Medium Composition ◽  
Regeneration Frequency ◽  
Optimum Result

Indica rice variety Ciherang is the most planted variety in Indonesia, but the micropropagation technique is restricted because it is known has low regeneration frequency and included as recalcitrant cultivar for tissue culture and tranformation activities. One of solution to resolve that problem is developing a technique of somatic embryogenesis in callus of ciherang rice cultivar. The aims of study were to determine medium composition for inducting embryogenic callus in Ciherang rice and to know the effectivity of rice regeneration using callus as explant. The methods were included induction of embryogenic callus in callus induction media (CIM) containing MS basal, Proline 600 mg/l, Casein Hidrolisat 300 mg/l, phytagel 2,5 g/l, BAP 0.25 mg/l, sukrosa 30 %, and 2,4-D in different concentration, from 2,4-D 0 mg/l as control (CIM 1), 2,4-D 2 mg/l (CIM 2), 2,4-D 3 mg/l (CIM 3), and 2,4-D 4 mg/l (CIM 4). It then be continued to regenerate the calli in RM 1 medium containing MS basal, NAA 0,2 mg/l, Kinetin 2 mg/l, Agarose 10 g/l, and sukrosa 30 %, pH 5,8 for six days in dark and RM2 medium containing MS basal , NAA 0,2 mg/l, Kinetin 2 mg/l, Agarose 8 g/l, sukrosa 30 %, pH 5,8 in light room. Results showed CIM 4 medium using 2,4-D 4 mg/l gave optimum result in calli induction with procentage 57,63% and CIM 3 using 2,4-D 3 mg/l gave optimum result in embryonic calli induction with procentage 53,63%. Result of embryonic calli gave regeneration frequency procentage is 9,6%. The total planlet obtained after five weeks old in regeneration medum were 11 planlets ready for aclimatization. Keywords: Somatic embryo, Indica rice (Oryza sativa L. var. Ciherang), Callus

scholarly journals Efficient Agrobacterium-mediated Transformation of The Elite–Indica Rice Variety Komboka

BIO-PROTOCOL ◽  
2020 ◽  
Vol 10 (17) ◽  
Author(s):  
Van Luu ◽  
Melissa Stiebner ◽  
Paula Maldonado ◽  
Sandra Valdés ◽  
Didier Marín ◽  
...  
Keyword(s):  
Indica Rice ◽  
Rice Variety ◽  
Agrobacterium Mediated Transformation
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