scholarly journals Influence of DNA treatments on Southern blot hybridization analysis of Fusarium oxysporum F. sp lycopersici and F. sp radicis-lycopersici genomic DNAs

2008 ◽  
Vol 7 (11) ◽  
pp. 1689-1692
Author(s):  
Samuel Balogun Olusegun
Keyword(s):  
Fusarium Oxysporum ◽  
Southern Blot ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Hybridization Analysis ◽  
Southern Blot Hybridization Analysis ◽  
Genomic Dnas

scholarly journals Southern blot-hybridization analysis of HBV DNA in patients with HBsAg seropositive chronic liver diseases.

Kanzo ◽  
1985 ◽  
Vol 26 (5) ◽  
pp. 565-571 ◽  
Author(s):  
Osamu YOKOSUKA ◽  
Masao OMATA ◽  
Fumio IMAZEKI ◽  
Yoshimi ITO ◽  
Junko MORI ◽  
...  
Keyword(s):  
Southern Blot ◽  
Liver Diseases ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Hbv Dna ◽  
Chronic Liver ◽  
Hybridization Analysis ◽  
Chronic Liver Diseases ◽  
Southern Blot Hybridization Analysis

scholarly journals Resistance to Bean pod mottle virus in Transgenic Soybean Lines Expressing the Capsid Polyprotein

2001 ◽  
Vol 91 (9) ◽  
pp. 831-838 ◽  
Author(s):  
M. S. Srinivasa Reddy ◽  
Said A. Ghabrial ◽  
Carl T. Redmond ◽  
Randy D. Dinkins ◽  
Glenn B. Collins
Keyword(s):  
Southern Blot ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Hybridization Analysis ◽  
Mottle Virus ◽  
Systemic Resistance ◽  
Transgenic Soybean ◽  
Bean Pod Mottle Virus ◽  
Southern Blot Hybridization Analysis ◽  
Soybean Plants

Transgenic fertile soybean plants were generated from somatic embryos of soybean (Glycine max) cv. Jack transformed via particle bombardment with the capsid polyprotein (pCP) gene of Bean pod mottle virus(BPMV). The plant transformation vector (pHIG/BPMV-pCP) utilized in these experiments contained the BPMV-pCP coding sequence, an intron-containing GUS gene, and the hygromycin phosphotransferase gene. Southern blot hybridization analysis showed that 19 transgenic soybean plants selected for resistance to hygromycin contained the genes for GUS and BPMV-pCP. The progeny of five of these transgenic soybean plants (plants 137, 139, 157, 183, and 186) were characterized in detail. An additional transgenic plant (plant 200) contained the intron-GUS and hygromycin resistance genes, but lacked the BPMV-pCP gene and was used as a negative control. Southern blot hybridization analysis of the five transgenic plants showed the presence of three copies of the T-DNA in a similar banding pattern suggesting that they were derived from a single transformation event. Western and northern blot analyses showed that the expression levels of BPMV-pCP and pCP transcript were high in these five pCP plants. Infectivity assays with detached leaves demonstrated that all five pCP plants exhibited resistance to virus infection because they accumulated lower levels of BPMV compared with plant 200 and nontransformed controls. Unlike the T2 progeny of line 183-1 that segregated with respect to the pCP gene and, consequently, to BPMV resistance, the T2 progeny of the homozygous line 183-2 showed little or no symptoms in response to rub-inoculation with virions of a severe strain of BPMV. Although BPMV accumulation was evident in leaves on which viruliferous beetles were allowed a 72-h inoculation access period, the upper noninoculated leaves of the T2 progeny of line 183-2 plants were symptomless and accumulated little or no virus. Because the progeny of this homozygous transgenic line exhibited systemic resistance, they could potentially be useful in generating commercial cultivars resistant to BPMV.

STABLE AND UNSTABLE MUTATIONS IN ABERRANT RATIO STOCKS OF MAIZE

Genetics ◽  
1984 ◽  
Vol 106 (4) ◽  
pp. 751-767
Author(s):  
John P Mottinger ◽  
Stephen L Dellaporta ◽  
Patrick B Keller
Keyword(s):  
Southern Blot ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Chromosome Breakage ◽  
Chromosome 9 ◽  
Dna Rearrangements ◽  
Transposition Activity ◽  
High Incidence ◽  
Southern Blot Hybridization Analysis ◽  
Unstable Mutations

ABSTRACT Aberrant Ratio (AR) stocks of maize were tested for transposition activity. Lines exhibiting AR and homozygous for the dominant alleles at the Sh Bz and Wx loci in the short arm of chromosome 9 were crossed as males to a sh bz wx tester. Among a population of 346,201 kernels, eight mutations of sh and two of bz were recovered. Eight of the ten mutations survived and none was as vigorous as its normal sibs. At least five of the sh mutants appear to be unstable in F2 and subsequent generations. An unexpected observation was the high incidence of somatic loss of chromosome 9 markers (Sh Bz and Wx), indicating chromosome breakage or nondisjunction. Southern blot hybridization analysis of the sh alterations indicate that all but one mutant are associated with structural DNA rearrangements at the shrunken locus. Possible mechanisms by which these alterations arose are discussed.

scholarly journals Length polymorphisms in human proline-rich protein genes generated by intragenic unequal crossing over.

Genetics ◽  
1988 ◽  
Vol 120 (1) ◽  
pp. 267-278 ◽  
Author(s):  
K M Lyons ◽  
J H Stein ◽  
O Smithies
Keyword(s):  
Dna Sequences ◽  
Tandem Repeats ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Length Variation ◽  
Length Polymorphisms ◽  
Dna Strands ◽  
Southern Blot Hybridization Analysis ◽  
Genomic Dnas ◽  
Proline Rich Protein

Abstract Southern blot hybridization analysis of genomic DNAs from 44 unrelated individuals revealed extensive insertion/deletion polymorphisms within the BstNI-type loci (PRB1, PRB2, PRB3 and PRB4) of the human proline-rich protein (PRP) multigene family. Ten length variants were cloned, including alleles at each of the four PRB loci, and in every case the region of length difference was localized to the tandemly repetitious third exon. DNA sequences covering the region of length variation were determined for seven of the alleles. The data indicate (1) that the PRB loci can be divided into two subtypes, PRB1 plus PRB2, and PRB3 plus PRB4, and (2) that the length differences result from different numbers of tandem repeats in the third exons. Variant chromosomes were also identified with different numbers of PRP loci resulting from homologous but unequal exchange between the PRB1 and PRB2 loci. The overall data are compatible with the observed length variants having been generated via homologous but unequal intragenic exchange. The results also indicate that these crossover events are sensitive to the amount of homology shared between the interacting DNA strands. Allelic length variants have arisen independently at least 20 times at the PRB loci, but only one has been detected at a PRH locus. Comparison of the detailed structures of the repetitious regions in PRB and PRH loci shows that the repeats in PRB genes are very similar to each other in sequence and in length. The PRH genes contain fewer repeats, which differ considerably in their individual lengths. These differences suggest that the larger number of length variants in PRB genes is related to their greater ease of homologous but unequal pairing compared to PRH genes.

Tandem arrangement of tubulin genes in the protozoan parasite Leishmania enriettii

1983 ◽  
Vol 3 (6) ◽  
pp. 1070-1076
Author(s):  
S M Landfear ◽  
D McMahon-Pratt ◽  
D F Wirth
Keyword(s):  
Tandem Repeat ◽  
Blot Hybridization ◽  
Protozoan Parasite ◽  
Southern Blot Hybridization ◽  
Beta Tubulin ◽  
Developmentally Regulated ◽  
Alpha Tubulin ◽  
Tubulin Genes ◽  
Southern Blot Hybridization Analysis ◽  
Leishmania Enriettii

The arrangement of developmentally regulated alpha- and beta-tubulin genes has been studied in the parasitic protozoan Leishmania enriettii by using Southern blot hybridization analysis. The alpha-tubulin genes occur in a tandem repeat whose monomeric unit may be represented by a 2-kilobase PstI fragment. Similarly, the beta-tubulin genes probably occur in a separate tandem repeat consisting of approximately 4-kilobase units unlinked to the alpha-tubulin repeats.

scholarly journals Tandem arrangement of tubulin genes in the protozoan parasite Leishmania enriettii.

1983 ◽  
Vol 3 (6) ◽  
pp. 1070-1076 ◽  
Author(s):  
S M Landfear ◽  
D McMahon-Pratt ◽  
D F Wirth
Keyword(s):  
Tandem Repeat ◽  
Blot Hybridization ◽  
Protozoan Parasite ◽  
Southern Blot Hybridization ◽  
Beta Tubulin ◽  
Developmentally Regulated ◽  
Alpha Tubulin ◽  
Tubulin Genes ◽  
Southern Blot Hybridization Analysis ◽  
Leishmania Enriettii

The arrangement of developmentally regulated alpha- and beta-tubulin genes has been studied in the parasitic protozoan Leishmania enriettii by using Southern blot hybridization analysis. The alpha-tubulin genes occur in a tandem repeat whose monomeric unit may be represented by a 2-kilobase PstI fragment. Similarly, the beta-tubulin genes probably occur in a separate tandem repeat consisting of approximately 4-kilobase units unlinked to the alpha-tubulin repeats.

Chromosomal Assignment of HepG2 3′-Directed Partial cDNA Sequences by Southern Blot Hybridization Using Monochromosomal Hybrid Cell Panels

Genomics ◽  
1994 ◽  
Vol 22 (1) ◽  
pp. 127-136 ◽  
Author(s):  
Atsushi Fukushima ◽  
Kousaku Okubo ◽  
Hidehiko Sugino ◽  
Naohiro Hori ◽  
Ryo Matoba ◽  
...  
Keyword(s):  
Southern Blot ◽  
Hybrid Cell ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Chromosomal Assignment ◽  
Cdna Sequences ◽  
Partial Cdna ◽  
Monochromosomal Hybrid
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