scholarly journals Genetic divergence in Agave accessions through ISSR markers and phenotypic traits

2018 ◽  
Vol 13 (11) ◽  
pp. 526-533 ◽  
Author(s):  
Chaves de Souza Silmara ◽  
Jaime Vasconcelos Cavalcanti Jose ◽  
Pierre Cordeiro Ramos Jean ◽  
Alves Isaías ◽  
Cavalcanti dos Santos Roseane ◽  
...  
Keyword(s):  
Genetic Divergence ◽  
Issr Markers ◽  
Phenotypic Traits

scholarly journals RAPD and ISSR markers in the evaluation of genetic divergence among accessions of elephant grass

2011 ◽  
Vol 10 (3) ◽  
pp. 1214-1223 ◽  
Author(s):  
R.S.N de Lima ◽  
R.F. Daher ◽  
L.S.A. Gonçalves ◽  
D.A. Rossi ◽  
A.T. do Amaral Júnior ◽  
...  
Keyword(s):  
Genetic Divergence ◽  
Issr Markers ◽  
Elephant Grass ◽  
Rapd And Issr

Genetic diversity of Achillea fragrantissima in Egypt inferred from phenotypic variations and ISSR markers associated with traits of plant size and seed yield

2016 ◽  
Vol 15 (3) ◽  
pp. 239-247 ◽  
Author(s):  
Abdelfattah Badr ◽  
Hanaa H. El-Shazly ◽  
Hanan I. Sayed Ahmed ◽  
Marwa Hamouda ◽  
Eman El-Khateeb ◽  
...  
Keyword(s):  
Seed Yield ◽  
Plant Size ◽  
Issr Markers ◽  
Gulf Of Aqaba ◽  
Phenotypic Traits ◽  
Mountainous Area ◽  
Achillea Fragrantissima ◽  
South Sinai ◽  
Lower Temperature ◽  
High Elevations

Substantial variation in phenotypic traits and ISSR fingerprinting was observed among twenty populations of Achillea fragrantissima (Forssk.) Sch. Bip. in Egypt. Such variation was reflected in the clustering of the examined populations into two major groups: one representing populations in the mountainous area of South Sinai and the other comprising populations growing at low elevations in the middle of Sinai and the desert west of the Suez Canal from Suez in the east to Cairo in the west. Five populations in the eastern part of Sinai near Nuwieba and Taba on the Gulf of Aqaba were loosely assigned to the first group. The populations growing at high elevations in South Sinai, under lower temperature and higher humidity, were characterized by a higher number of total and polymorphic ISSR markers compared with other populations. Unique ISSR markers were more often observed in the fingerprinting of seven populations including five populations growing in the high mountains of Saint Catherine in South Sinai and two populations growing at low elevations but at Wadi Hof south-east of Cairo. Interestingly, unique bands were found in the populations that possessed traits associated with larger plant size and seed yield as well as better vigour. These are important criteria for the selection of A. fragrantissima populations for conservation and sustainable commercial use.

scholarly journals Evidence for continual hybridization rather than hybrid speciation betweenLigularia duciformisandL. paradoxa(Asteraceae)

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3884 ◽  
Author(s):  
Rong Zhang ◽  
Xun Gong ◽  
Ryan Folk
Keyword(s):  
Parental Species ◽  
Reproductive Traits ◽  
Issr Markers ◽  
Natural Hybridization ◽  
Hybrid Origin ◽  
Phenotypic Traits ◽  
Hybrid Species ◽  
Network Analyses ◽  
Hybrid Swarms ◽  
Ssr Loci

BackgroundHybrids possess phenotypic traits that are often intermediate between their parental taxa, which commonly serves as evidence of hybridization in morphological analyses. Natural hybridization has been shown to occur frequently inLigularia(Asteraceae). In a previous study,Ligularia×maoniushanensiswas demonstrated as a natural hybrid species betweenL. duciformisandL.paradoxabased on morphological and reproductive traits.MethodsWe used three chloroplast (cpDNA) fragments (psbA-trnH,trnL-rpl32 andtrnQ-5′rps16), the nuclear ribosomal internal transcribed spacer (nrITS), and co-dominant SSR and dominant ISSR markers to study natural hybridization betweenL. duciformisandL. paradoxagrowing sympatrically in two locations. Parental taxa were inferred using network analyses of cpDNA and nrITS haplotypes. Admixture among individuals was examined using the Bayesian clustering programs STRUCTURE and NewHybrids based on the SSR and ISSR data; and potential introgression in the SSR loci was assessed using the INTROGRESS package.ResultsThe putative parental species were clearly distinguished from other sympatricLigulariaspecies by nrITS data, andL.×maoniushanensisindividuals were confirmed to be the hybrid offspring ofL.duciformisandL.paradoxa. Moreover, introgression was detected among several individuals morphologically identified asL.duciformisorL. paradoxa. Analyses of the cpDNA data revealed primarily unidirectional hybridization betweenL. duciformisandL. paradoxa, withL.paradoxaas the maternal parent in Mt. Maoniu, whereas bidirectional but asymmetrical hybridization was inferred to occur in Heihai Lake. The STRUCTURE analyses based on the SSR data detected two distinct clusters among the three taxa. The NewHybrids analyses showed that individuals circumscribed asL.×maoniushanensiswere dominated by early- and later-generation and backcrossing hybrids. The NewHybrids results based on the ISSR data were congruent with SSR results. In addition, introgression was detected in some SSR loci, and heterogeneity among loci was found in terms of detected patterns of introgression.ConclusionsOur data provide strong evidence for hybridization and introgression betweenL.duciformisandL.paradoxa.Ligularia×maoniushanensiswas demonstrated to be of hybrid origin. Since no evident reproductive isolation was found between the two parental species, detected hybrids appear to be part of hybrid swarms resulting from frequent and ongoing gene flow, which might impede the formation of a new hybrid species.

scholarly journals Genetic Divergence Detected by ISSR Markers and Characterization of Microsatellite Regions in Mytilus Mussels

2007 ◽  
Vol 45 (7-8) ◽  
pp. 565-578 ◽  
Author(s):  
Miguel A. Varela ◽  
Ana González-Tizón ◽  
Luis Mariñas ◽  
Andrés Martínez-Lage
Keyword(s):  
Genetic Divergence ◽  
Issr Markers

scholarly journals Genetic divergence in soybeans based on phenotypic traits and microsatellite markers

2014 ◽  
Vol 8 (S4) ◽  
Author(s):  
Larissa Barbosa de Sousa ◽  
Ana Paula Oliveira Nogueira ◽  
Osvaldo Toshiyuki Hamawaki ◽  
Ana Carolina Cordeiro Dias ◽  
Dão Pedro de Carvalho Neto ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Genetic Divergence ◽  
Phenotypic Traits

scholarly journals MOLECULAR CHARACTERIZATION OF JABOTICABA TREE GENOTYPES LOCATED IN THE MUNICIPALITIES OF RECÔNCAVO OF BAHIA

2016 ◽  
Vol 38 (3) ◽  
Author(s):  
ELAINE SILVA DA CRUZ ◽  
ANA CRISTINA VELLO LOYOLA DANTAS ◽  
CÁTIA DIAS DO CARMO ◽  
LUCIMÁRIO PEREIRA BASTOS
Keyword(s):  
Genetic Divergence ◽  
Polymorphic Information Content ◽  
Issr Markers ◽  
Fruit Tree ◽  
Resolution Power ◽  
Inter Simple Sequence Repeats ◽  
Native Populations ◽  
Simple Sequence ◽  
Issr Primers

ABSTRACT The jaboticaba tree (Plinia sp.) is a native fruit tree belonging to the Myrtaceae family, which occurs spontaneously throughout Brazil. The estimation of genetic divergence among genotypes of native populations can be useful for conservation and knowledge of available genetic resources, in the formation of gene banks and breeding species. In order to evaluate the genetic diversity of jaboticaba trees identified in the Bahia Reconcavo municipalities, 35 genotypes were characterized by ISSR primers (Inter Simple Sequence Repeats). The 18 primers generated a total of 463 amplicons, and the number of initiator fragments ranged from 14 to 36, with an average of 25.72% and 99.65% polymorphism. Polymorphic information content ranged from 0.13 to 0.33 with a mean of 0.22 and the marker resolution power from 4.34 to 15.77, with an average of 8.67. Multivariate analysis allowed the formation of five groups of genetic divergence, where the longest distance was 0.97 between JCA6 and JSF8 genotypes and the lower of JMT2 and JCA1 (0.11). From the use of ISSR markers it is possible to check the variability between genotypes on the evaluated jaboticaba trees.

scholarly journals Evaluation of diversity in Bulgarian pepper cultivars by agronomical traits and ISSR markers

Genetika ◽  
2017 ◽  
Vol 49 (2) ◽  
pp. 647-662 ◽  
Author(s):  
Stefan Tsonev ◽  
Velichka Todorova ◽  
Stanislava Groseva ◽  
Teodora Popova ◽  
Elena Todorovska
Keyword(s):  
Agronomic Traits ◽  
Phenotypic Diversity ◽  
Issr Markers ◽  
Genetic Distances ◽  
Two Dimensions ◽  
Phenotypic Characterization ◽  
Phenotypic Traits ◽  
Vegetable Crops ◽  
Large Collection ◽  
Positive Direction

Information about the genetic variation among cultivars of vegetable crops is of vital importance for improvement of plant breeding programmes worldwide. The objectives of this study were to group 19 pepper (Capsicum annuum L.) cultivars from the collection of Maritsa Vegetable Crops Research Institute, Plovdiv, Bulgariainto clusters according to their distances as estimated by agronomic traits and 9 di-and tri -nucleotide inter simple sequence repeat polymorphism (ISSR) markers and to assess the relationships between them. The phenotypic characterization during 3 consecutive years revealed significant differences among Bulgarian cultivars for the studied 13 phenotypic traits. The biplot analysis of quantitative traits showed that the most strongly correlating traits with the first axis (55.6% of variance) were fruit width, fruit weight and pericarp thickness (in the negative direction of the axis), and plant height (PH) (in the positive direction). The most discriminative traits, considering the second axis (22.6% of variance) were fruit length (FL) and to a lesser extent the stem height (StH). The correspondence analysis of the qualitative traits showed that the intensity of the colour of the fruit (before and at maturity), fruit colourbefore maturity and fruit shape in longitudinal section were the most discriminative characteristics for the first two dimensions. The agronomic traits data and 7 dinucleotide ISSR primers were used to estimate the pairwise genetic distances. Higher mean phenotypic distance (0.414) in comparison to the genotypic ones (0.214) among the cultivars was observed, indicating higher phenotypic diversity among them. A highly signi?cant, positive correlation between the agronomic data and ISSR marker-based matrices (r=0.41, p=0.001)was detected. This indicates that ISSR distance tended to re?ect that of the agronomics ones. However, additional molecular studies and large collection of highly diverse genotypes are needed to reveal associations between the marker loci and specific QTLs. The research initiated is a base for more precise estimation of genetic distances between pepper genotypes from the available large collection of landraces, local and modern cultivars including large number of highly polymorphic markers.

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