scholarly journals In vitro efficacy of certain botanicals against bacterial soft rot of tomato (Solanum lycopersicum L.)

2017 ◽  
Vol 12 (23) ◽  
pp. 2049-2055
Author(s):  
S. H. Adamu ◽  
A. A. Lal ◽  
S. Simon
Keyword(s):  
Solanum Lycopersicum ◽  
Soft Rot ◽  
Bacterial Soft Rot ◽  
Solanum Lycopersicum L

Морфо-биологическая и цитологическая характеристика регенерирующих корней томата ( Solanum lycopersicum L.) сорта “Рекордсмен” в условиях NaCl-засоления in vitro

Tsitologiya ◽  
2019 ◽  
Vol 61 (12) ◽  
pp. 998-1013
Author(s):  
Л. Р. Богоутдинова ◽  
Е. Н. Баранова ◽  
Г. Б. Баранова ◽  
Н. В. Кононенко ◽  
Е. М. Лазарева ◽  
...  
Keyword(s):  
Solanum Lycopersicum ◽  
Solanum Lycopersicum L

scholarly journals Impact of Glucosinolate Content in Broccoli (Brassica oleracea (Italica Group)) on Growth of Pseudomonas marginalis, a Causal Agent of Bacterial Soft Rot

Plant Disease ◽  
2002 ◽  
Vol 86 (6) ◽  
pp. 629-632 ◽  
Author(s):  
Craig S. Charron ◽  
Carl E. Sams ◽  
Craig H. Canaday
Keyword(s):  
Brassica Oleracea ◽  
Degradation Products ◽  
Linear Regression Analysis ◽  
Soft Rot ◽  
Causal Agent ◽  
Glucosinolate Content ◽  
Bacterial Soft Rot ◽  
Vitro Assay ◽  
The Impact

Glucosinolate degradation products are known to suppress microbes. Brassica species produce glucosinolates. Previous investigations determined that susceptibility to bacterial soft rot of broccoli (Brassica oleracea (Italica group)) varied significantly by cultivar. To evaluate the impact of glucosinolates on Pseudomonas marginalis, a causal agent of bacterial soft rot, glucosinolates were measured in lyophilized florets from broccoli ‘Arcadia’, ‘Emperor’, ‘Green Comet’, ‘Green Valiant’, ‘Marathon’, ‘Packman’, ‘Premium Crop’, and ‘Shogun’. Total glucosinolate content was highest in ‘Shogun’ (29.8 μmol/g) and lowest in ‘Emperor’ (0.5 μmol/g). In an in vitro assay, simple linear regression analysis showed that 48% of differences in suppression of P. marginalis growth could be explained by differences in total glucosinolate content (P ≤ 0.01). Plant breeding efforts should include glucosinolate levels as a factor in selecting for disease resistance.

scholarly journals Trichoderma harzianum como herramienta en el manejo integrado de enfermedades de tomate

2021 ◽  
Author(s):  
◽  
Román Müller
Keyword(s):  
Solanum Lycopersicum ◽  
Trichoderma Harzianum ◽  
Sclerotium Rolfsii ◽  
Trichoderma Spp ◽  
Solanum Lycopersicum L ◽  
Nuevas Tecnologías

En nuestro país como a nivel mundial, el tomate (Solanum lycopersicum L. = Lycopersicun esculetum Mill.), es una de las hortalizas más consumida y de mayor valor económico. Durante su cultivo, se encuentra expuesto a distintas plagas, entre ellas a Sclerotium rolfsii. Para un control eficiente de las enfermedades es conveniente utilizar un manejo integrado de plagas, utilizando distintas técnicas de cultivo tanto clásicas como nuevas tecnologías como el control biológico, que contemplen paralelamente el cuidado del medio ambiente y la producción del cultivo. Trichoderma spp., es uno de los organismos más utilizados para el control biológico de las enfermedades de origen fúngico. El objetivo de este trabajo fue evaluar la eficacia de la cepa de Trichoderma harzianum del IFSC como controlador biológico de Sclerotium rolfsii, ya sea sola o con fungicidas comerciales y analizar su capacidad como promotora de crecimiento del cultivo de tomate platense variedad Luna. Se evaluó el comportamiento de Th del IFSC para controlar a Sclerotium rolfsii in vitro, en cámara de crecimiento y a campo. Como resultado de los ensayos se comprobó que la cepa de Th del IFSC incrementó la germinación en condiciones in vitro e inhibió el desarrollo de S. rolfsii, poniéndose en evidencia el mecanismo de destrucción de los esclerocios. Indujo la promoción del crecimiento de la plántula obteniéndose plantas con mayor sanidad y porte. La cepa Th del IFSC tuvo mayor afinidad con el fungicida Captan incrementando la germinación y el desarrollo de las plántulas en el cultivo de tomate. Se comprobó la factibilidad del uso de Th del IFSC en un planteo de manejo integrado en el cultivo de tomate platense variedad Luna ya sea sola o con un fungicida como Captan.

scholarly journals Вплив похідних піразолу на ріст і розвиток рослин в умовах in vivo та in vitro

2021 ◽  
pp. 108-119
Author(s):  
І.В. Могільнікова ◽  
В.А. Циганкова ◽  
А.О. Гуренко ◽  
В.С. Броварець ◽  
Н.М. Білько ◽  
...  
Keyword(s):  
Solanum Lycopersicum ◽  
Solanum Lycopersicum L

Досліджено вплив синтетичних низькомолекулярних сполук — похідних піразолу — на ріст і розвиток рослин томата (Solanum lycopersicum L.) сорту Money Maker в умовах in vivo та in vitro. Встановлено, що найбільш виражену активність в умовах in vivo виявляє сполука D-pyrazole-3 у концентрації 10–8 М. Вперше досліджено вплив цих сполук на морфогенетичний потенціал експлантів S. lycopersicum в умовах in vitro і показано, що сполуки D-pyrazole-1, D-pyrazole-2 і D-pyrazole-3 ефективно індукують калюсогенез та ризогенез, а також, залежно від концентрації, пряму регенерацію рослин в умовах in vitro. Під дією всіх трьох сполук як у концентрації 10–9 М, так і 10–8 М найефективніше коренеутворення відбувається на пагонових експлантах. Для прямої регенерації рослин із цього типу експлантів найбільш ефективними є сполуки D-pyrazole-1 у концентрації 10–9 М або D-pyrazole-2 у концентрації 10–8 М, які в подальшому можуть бути рекомендовані для використання в біотехнологічних дослідженнях з генетичного вдосконалення S. lycopersicum.

FUNGITOXICIDADE DO ÓLEO ESSENCIAL DE Lippia alba SOBRE Corynespora cassiicola ISOLADO DE TOMATEIRO

2021 ◽  
Author(s):  
João Paulo Fonseca Tavares ◽  
João Bosco Batista Nogueira Júnior ◽  
Ítalo Fernando da Costa Melo
Keyword(s):  
Solanum Lycopersicum ◽  
Corynespora Cassiicola ◽  
Solanum Lycopersicum L ◽  
Lippia Alba

Introdução: O tomate (Solanum lycopersicum L.) é uma das principais culturas hortícolas em todo o mundo devido à sua importância nutricional e econômica. Entretanto, alguns problemas fitossanitários limitam o cultivo dessa hortaliça. Na região Norte do país, a mancha-alvo causada pelo fungo Corynespora cassiicola está entre as doenças mais importantes que atacam a parte aérea do tomateiro, ocasionando perda da produtividade dessa hortaliça. Objetivo: Avaliar o potencial do óleo essencial de Lippia alba. Material e métodos: A avaliação in vitro foi realizada em duas etapas: I - efeito dos óleos essenciais sobre o crescimento micelial, II - ação dos óleos sobre a esporulação e germinação de conídios do fungo. Foram utilizadas as concentrações: 1,2% (900 µL), 1,0% (750 µL), 0,7% (525 µL), 0,5% (375 µL), 0,2% (150 µL) e 0,0% (0,0 µL), sendo esta última o controle negativo. Resultados: A utilização de óleo essencial de Lippia alba na inibição do crescimento micelial (ICM), foi acima de 100% para as concentrações: 05%, 0,7%, 1,0% e 1,2%. Na concentração de 0,2%, houve uma inibição de 52,5% do crescimento micelial. O controle obteve valores de diâmetro de colônia em tono de 8 cm. No ensaio in vitro da porcentagem de inibição da esporulação (PIE), houve 100% da inibição para as concentrações de 05% a 1,2% e uma inibição de 52,9% para a concentração de 0,2%. Quanto à porcentagem de inibição da germinação, em todas as concentrações avaliadas, houve inibição de 100% dos conídios, apresentando efeito fungistático. Conclusão: O óleo essencial de L. alba apresenta atividade antifúngica inibindo tanto o crescimento micelial de C. cassiicola, quanto a esporulação e germinação do patógeno. Concentrações a partir de 375µL, reduz significativamente o desenvolvimento do fungo.

scholarly journals Original Article. In vitro evaluation of potato genotypes for resistance against bacterial soft rot (Pectobacterium carotovorum) – a new tool for studying disease resistance

2016 ◽  
Vol 57 (1) ◽  
pp. 1-8
Author(s):  
Sima Azadmanesh ◽  
Javad Mozafari ◽  
Nader Hasanzadeh ◽  
Cobra Moslemkhani
Keyword(s):  
Soft Rot ◽  
Bacterial Suspension ◽  
Post Inoculation ◽  
Pectobacterium Carotovorum ◽  
In Vitro Screening ◽  
Bacterial Soft Rot ◽  
Inoculation Techniques ◽  
Potato Collection ◽  
Rot Disease

Abstract In vitro screening techniques were used to evaluate 46 genotypes of Iranian potato collection for resistance to bacterial soft rot caused by Pectobacterium carotovorum subsp. carotovorum (Pcc). One month old in vitro rooted potato plantlets were inoculated by two inoculation techniques under in vitro conditions: 1) sterile toothpicks dipped into bacterial suspension and pressed into the crown of plantlets and 2) the freshly cut crown of plantlets were dipped into bacterial suspension of 108 cfu ∙ ml-1 for 10 min. Typical soft rot disease symptoms, including the percentage of wilted leaves were recorded on inoculated plantlets 3, 6, 9, 12 and 15 days post-inoculation. The potato genotypes which were examined responded differently to Pcc and varying levels of resistance were observed. Potato genotype AG showed the highest level of resistance. Results obtained from in vitro screening were then verified by classical tuber slice assay. The verifications were conducted using five representative cultivars: Milva, Ramus, Picaso, Marfona and Agria which responded similarly to both in vitro and classical evaluation systems. Similar results obtained from these tests indicated that the in vitro screening technique developed in this study could provide a simple and rapid whole plant assay in selecting resistant potato genotypes against bacterial soft rot.

scholarly journals Induction and Growth Kinetics Callus of Tomato (Solanum lycopersicum)

2020 ◽  
Vol 12 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Arkan Setiaji ◽  
RR Rifka Annisa ◽  
Rumiyati Rumiyati ◽  
Endang Semiarti
Keyword(s):  
Solanum Lycopersicum ◽  
Growth Kinetics ◽  
Callus Induction ◽  
Dry Weight ◽  
Growth Efficiency ◽  
Ms Medium ◽  
Solanum Lycopersicum L ◽  
Absolute Growth ◽  
Embedded Method

Plant callus extracts are potential to be developed as ingredient in skincare products. Tomato callus is supposed to contain protein-derivatives and or other components such as secondary metabolites that play a role in skin regeneration. Therefore the production of calli is important to be studied for callus sustainable supply. This research aims to obtain optimum medium for callus induction and to analyze tomato callus development anatomically. In vitro culture response was assessed in tomato plant (Solanum lycopersicum L. ‘Permata’) for optimum callus induction. Seeds were grown on ¼ MS medium for 10-15 days. Hypocotyl was excised and cultured on MS medium + 2 mg/l 2,4-D for 15 days as the explants for callus induction. Callus was transferred to MS medium with 8 variations of PGRs including the combination of BAP + NAA, and 2,4-D. Both fresh and dry weight was measured every 5 days over 60 days to establish the growth kinetics and growth efficiency of callus. Anatomic characters of calli were examined through paraffin-embedded method. The result showed of MS medium supplemented with 2.0 mg/l NAA and 0.2 mg/l BAP is optimum for tomato callus induction, based on highest number of the absolute growth rate on fresh weight (73.77% per day), dry weight (3.84% per day), and callus initiation time (5.56 days) achieved by the medium. Cells in the ground tissue of tomato hypocotyl are competent to be dedifferentiated into a callus. This research results were expected to find out suitable methods for tomato callus production in preparation for skincare uses.

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