scholarly journals The effect of nitrogen supply on potato yield, tuber size and pathogen resistance in Solanum tuberosum exposed to Phytophthora infestans

2014 ◽  
Vol 9 (35) ◽  
pp. 2657-2663 ◽  
Author(s):  
Jin Xuehui ◽  
Hao Na ◽  
Jiao Feng ◽  
Yang Yang ◽  
Wang Dan ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Phytophthora Infestans ◽  
Pathogen Resistance ◽  
Potato Yield ◽  
Nitrogen Supply ◽  
Tuber Size

Localization of Ds-transposon containing T-DNA inserts in the diploid transgenic potato: linkage to the R1 resistance gene against Phytophthora infestans (Mont.) de Bary

Genome ◽  
1996 ◽  
Vol 39 (2) ◽  
pp. 249-257 ◽  
Author(s):  
A. El-Kharbotly ◽  
J. M. E. Jacobs ◽  
B. te Lintel Hekkert ◽  
W. J. Stiekema ◽  
A. Pereira ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Phytophthora Infestans ◽  
Resistance Gene ◽  
Dna Sequences ◽  
Transgenic Potato ◽  
Potato Genome ◽  
Rflp Map ◽  
Polymerase Chain ◽  
R1 Gene ◽  
Map Location

The Dissociation transposable element (Ds) of maize containing NPTII was introduced into the diploid potato (Solanum tuberosum) clone J91-6400-A16 through Agrobacterium tumefaciens mediated transformation. Genomic DNA sequences flanking the T-DNAs from 312 transformants were obtained with inverse polymerase chain reaction or plasmid rescue techniques and used as probes for RFLP linkage analysis. The RFLP map location of 60 T-DNAs carrying Ds–NPTII was determined. The T-DNA distribution per chromosome and the relative distance between them appeared to be random. All 12 chromosomes have been covered with Ds-containing T-DNAs, potentially enabling tagging of any gene in the potato genome. The T-DNA insertions of two transformants, BET92-Ds-A16-259 and BET92-Ds-A16-416, were linked in repulsion to the position of the resistance gene R1 against Phytophthora infestans. After crossing BET92-Ds-A16-416 with a susceptible parent, 4 desired recombinants (Ds carrying T-DNA linked in coupling phase with the R1 gene) were discovered. These will be used for tagging the R1 gene. The efficiency of the pathway from the introduction to localization of T-DNAs is discussed. Key words : Solanum tuberosum, Phytophthora infestans, Ds element, transposon tagging, R genes, euchromatin.

scholarly journals Respuesta de biosurfactantes producidos por Pseudomonas fluorescens para el control de la gota de la papa Phytophthora infestans (Mont) de Bary, bajo condiciones controlada.

2010 ◽  
Vol 11 (1) ◽  
pp. 21
Author(s):  
Hugo F. Rivera ◽  
Erika P. Martínez ◽  
Jairo A. Osorio ◽  
Edgar Martínez
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Pseudomonas Fluorescens ◽  
Negative Impact ◽  
Potato Late Blight ◽  
Pathogen Resistance ◽  
Production Costs ◽  
In Vitro Assays

<p>Phytophthora infestans (Mont.) de Bary, agente causal de la gota de la papa, es considerado la principal limitante de la producción de este cultivo en Colombia. El control habitual del patógeno se realiza con fungicidas de tipo sistémico, que incrementan los costos de producción, pueden inducir la resistencia del patógeno y tiene un impacto negativo en el ambiente. Por tanto, se llevó a cabo este estudio con el propósito de buscar alternativas amigables con el ambiente, que hagan parte de un paquete tecnológico eficaz de control. Dos cepas nativas de Psedomonas fluorescens (039T y 021V), provenientes de cultivos de papa, fueron evaluadas contra P. infestans. Las suspensiones bacterianas y los biosurfactantes parcialmente purificados (BPP), producidos por éstas (obtenidos en medio mínimo de sales con querosén), fueron aplicados sobre foliolos desprendidos en ensayos in vitro y experimentos in vivo en plantas de papa, en condiciones controladas en casa de malla. Los resultados demostraron la capacidad que tienen los biosurfactantes y las suspensiones bacterianas para controlar al patógeno, ya que el BPP 039T logró reducir el nivel de severidad de la enfermedad en 79,9% in vitro y 38,5% in vivo, mientras que el BPP 021V redujo en 78,7% in vitro y 30,2% in vivo. Las suspensiones bacterianas redujeron el nivel de severidad en 72,4% (039T) y 66,1% (021V) en las evaluaciones in vitro y 35% en los experimentos in vivo. Los resultados de esta investigación muestran el potencial que tienen los biosurfactantes para el control de la gota en Colombia.</p><p> </p><p><strong>Evaluation of Biosurfactants Produced by Pseudomonas fluorescens for Potato Late Blight Control (Phytophthora infestans (Mont) de Bary) Under Controlled Conditions</strong></p><p>Phytophthora infestans (Mont.) de Bary, causal agent of potato late blight is considered the main limiting pathogen for the production of this crop in Colombia. The usual control of the disease has been performed with systemic fungicides which increase production costs, can induce pathogen resistance and have a negative impact on the environment. Therefore, this study was carried out in order to find effective and environmentally friendly control alternatives for potato late blight. Two Pseudomonas fluorescens native strains (039T and 021V) isolated from potato crops were evaluated against P. infestans. Bacterial suspensions (obtained from minimal salts medium added with kerosene) and partially purified biosurfactants (BPP) were applied on detached leaflets for in vitro assays and on potato plants in greenhouse, for in vivo assays and the measure of inhibitory effect of the disease was assessed. The results showed the ability of P. fluorescens biosurfactants and bacterial suspensions to control the pathogen. BPP 039T was able to reduce the level of severity disease by 79.9% in vitro and 38.5% in vivo, whereas BPP 021V decreased 78.7% in vitro and 30.2% in vivo. Bacterial suspensions reduced the severity level in 72.4% (039T) and 66.1% (021V) in vitro assessments and 35% in the in vivo experiment. These results show the potential of P. fluorescens biosurfactants to control the potato late blight in Colombia.</p>

scholarly journals Determinación de Razas Fisiológicas y Tipo de Apareamiento en Aislamientos de Phytophthora infestans (Mont) De Bary

2016 ◽  
Vol 9 (1) ◽  
pp. 156-170
Author(s):  
Juan Bautista López ◽  
María Elena Márquez ◽  
Sonia Jaramillo ◽  
José Luís Zapata
Keyword(s):  
Solanum Tuberosum ◽  
Lycopersicon Esculentum ◽  
Phytophthora Infestans ◽  
Solanum Phureja ◽  
Solanum Muricatum

El problema más limitante del cultivo de la papa en muchas regiones del mundo, lo constituye la gota o tizón tardío ocasionado por el hongo Phytophthora infestans. Esta investigación se realizó con el objetivo de determinar variaciones poblacionales de este patógeno, basadas en el tipo de apareamiento y en la presencia de razas fisiológicas en 19 aislamientos de P. infestans obtenidos en plantas de papa (Solanum tuberosum) variedades Diacol Capiro, ICA Puracé e ICA Cumanday; papa criolla (Solanum phureja), pepino (Solanum muricatum) y tomate (Lycopersicon esculentum) en diferentes zonas del Departamento de Antioquia (Colombia).Los resultados obtenidos a partir de un apareamiento patrón A1, con los aislamientos colectados en las diferentes zonas sugieren la existencia de una población de P. infestans con un solo tipo de apareamiento. La prueba de virulencia del hongo, realizada en folíolos de 11 diferenciales de papa mostró la presencia de 6 razas fisiológicas, de las cuales la más frecuente fue la 7446 (58%), con los factores de virulencia 1-2-3-4-7-10-11. Aislamientos monozoospóricos presentaron los mismos factores de virulencia que sus respectivos aislamientos recolectados en el campo, lo que sugiere homogeneidad de la población fungosa. La gran complejidad de las razas fisiológicas de P. infestans podría estar influenciada por diversos factores genéticos y medioambientales.Aceptado para publicación: mayo 1997.

scholarly journals QTL analysis reveals quantitative resistant loci for Phytophthora infestans and Tecia solanivora in tetraploid potato (Solanum tuberosum L.)

PLoS ONE ◽  
2018 ◽  
Vol 13 (7) ◽  
pp. e0199716 ◽  
Author(s):  
Juan David Santa ◽  
Jhon Berdugo-Cely ◽  
Liliana Cely-Pardo ◽  
Mauricio Soto-Suárez ◽  
Teresa Mosquera ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Phytophthora Infestans ◽  
Qtl Analysis ◽  
Solanum Tuberosum L ◽  
Tetraploid Potato ◽  
Tecia Solanivora

scholarly journals Variedades de batatinha (Solanum tuberosum L.). I - Comportamento de 12 variedades procedentes da Holanda, Alemanha e Suécia

Bragantia ◽  
1956 ◽  
Vol 15 (unico) ◽  
pp. 153-168
Author(s):  
O. J. Boock
Keyword(s):  
Solanum Tuberosum ◽  
Phytophthora Infestans ◽  
Solanum Tuberosum L ◽  
Alternaria Solani ◽  
Sao Paulo ◽  
São Paulo

No presente trabalho são relatadas experiências com 12 variedades de batatinha (Solanum tuberosum L.), recebidas da Holanda (Prinslander, Irene, Froma e Barima), Alemanha (Merkur, Sabina, Linda e Concordia) e Suécia Konsuragis, Eigenheimer, Voran e Jätte-Bintje). Essas experiências, em número de seis, das quais três no chamado período "da sêca" (março-julho) e três no "das águas" (setembro-janeiro), foram executadas nas localidades de Campinas, Louveira o Capão Bonito, no Estado de São Paulo. Ficou evidenciado o seguinte: a) dado o bom estado de brotação dos tubérculos, as porcentagens de falhas no plantio "da sêca" foram baixas; já no plantio "das águas", as variedades de brotação lenta, como "Voran" e "Sabina", falharam muito; b) "Prinslander" e "Konsuragis" resistiram bem à sêca prolongada, e "Barima" o "Linda" mostraram ser muito sensíveis; c) "Linda", "Voran" e "Merkur", principalmente a primeira destas, apresentaram boa resistência a Phytophthora infestans, e "Jätte-Bintje" foi muito suscetível; "Voran", "Irene", "Prinslander" e "Barima" não ofereceram resistência a Alternaria solani; d) "Merkur" e "Konsuragis" foram as mais produtivas para as duas épocas de plantio, sendo que "nas águas", "Eigenheimer", "Barima", "Concordia" e Jätte-Bintje", também produziram bem. Outros aspectos relacionados com variedades foram estudados.

Effect of Haloxyfop on Quackgrass (Elytrigia repens) and Potatoes (Solanum tuberosum)

1991 ◽  
Vol 5 (1) ◽  
pp. 72-75 ◽  
Author(s):  
Jerry A. Ivany
Keyword(s):  
Adverse Effect ◽  
Solanum Tuberosum ◽  
Potato Yield ◽  
No Removal ◽  
Leaf Stage ◽  
Application Rates ◽  
Elytrigia Repens

Reduction of initial top growth of quackgrass with haloxyfop at 0.13 kg ai ha-1and above in the greenhouse was comparable at the 2-, 4-, and 6-leaf stages. Quackgrass control was not affected by rhizome length in plants grown from 2-node and 10-node rhizome pieces. Removal of quackgrass shoots 6 h after treatment resulted in increased initial topgrowth, regrowth, and rhizome weight at all application rates when compared to treatments with no removal of shoots; however, growth decreased with increasing rates of haloxyfop. Later removal at 24 or 96 h after treatment did not affect quackgrass control, indicating rapid translocation. In field-grown potato, haloxyfop applied at 0.25 kg ha-1at the 4- to 5-leaf stage controlled more than 80% of quackgrass. Lower rates controlled less than 80% when used alone; but adding cultivation improved control. No adverse effect on potato yield was noted with haloxyfop.

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