A highly efficient and improved protocol for exploitation of somaclonal variation for enhancing alien gene introgression in wide cross hybrid of Oryza sativa/Oryza brachyantha

K. Bose L.; Kar M.; Bhattacharya B.; N. Jambhulkar N.; N. Singh O.

doi:10.5897/ajar12.2208

A highly efficient and improved protocol for exploitation of somaclonal variation for enhancing alien gene introgression in wide cross hybrid of Oryza sativa/Oryza brachyantha

African Journal of Agricultural Research ◽

10.5897/ajar12.2208 ◽

2014 ◽

Vol 9 (2) ◽

pp. 262-268

Author(s):

K. Bose L. ◽

Kar M. ◽

Bhattacharya B. ◽

N. Jambhulkar N. ◽

N. Singh O.

Keyword(s):

Oryza Sativa ◽

Somaclonal Variation ◽

Gene Introgression ◽

Wide Cross ◽

Highly Efficient ◽

Oryza Brachyantha ◽

Alien Gene

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Development and validation of cross-transferable and polymorphic DNA markers for detecting alien genome introgression in Oryza sativa from Oryza brachyantha

Molecular Genetics and Genomics ◽

10.1007/s00438-016-1214-z ◽

2016 ◽

Vol 291 (4) ◽

pp. 1783-1794 ◽

Cited By ~ 6

Author(s):

Soham Ray ◽

Lotan K. Bose ◽

Joshitha Ray ◽

Umakanta Ngangkham ◽

Jawahar L. Katara ◽

...

Keyword(s):

Oryza Sativa ◽

Dna Markers ◽

Oryza Brachyantha ◽

Development And Validation

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Cytological characterization of anther culture derived plants from the interspecific crosses between Oryza sativa x Oryza australinesis and Oryza sativa x Oryza brachyantha

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb10.1696 ◽

2011 ◽

Vol 10 (17) ◽

pp. 3269-3273 ◽

Cited By ~ 1

Author(s):

M Abbasi Fida ◽

Akbar Kehkashan ◽

Ur Rehman Mujaddad ◽

Tariq Khan Muhammad ◽

Iqbal Sajjad ◽

...

Keyword(s):

Oryza Sativa ◽

Anther Culture ◽

Interspecific Crosses ◽

Oryza Brachyantha

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RFLP Linkage Map included the Information of Segregation Distortion in a Wide-Cross Population between Indica and Japonica Rice (Oryza sativa L.).

Breeding Science ◽

10.1270/jsbbs.50.65 ◽

2000 ◽

Vol 50 (2) ◽

pp. 65-72 ◽

Cited By ~ 10

Author(s):

Yoshimichi Fukuta ◽

Hideki Sasahara ◽

Katsunori Tamura ◽

Toshinori Fukuyama

Keyword(s):

Oryza Sativa ◽

Linkage Map ◽

Segregation Distortion ◽

Oryza Sativa L ◽

Japonica Rice ◽

Wide Cross

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Dynamics of the Methylome and Transcriptome during the Regeneration of Rice

Epigenomes ◽

10.3390/epigenomes2030014 ◽

2018 ◽

Vol 2 (3) ◽

pp. 14 ◽

Cited By ~ 2

Author(s):

Fei-Man Hsu ◽

Moloya Gohain ◽

Archana Allishe ◽

Yan-Jiun Huang ◽

Jo-Ling Liao ◽

...

Keyword(s):

Dna Methylation ◽

Oryza Sativa ◽

Somaclonal Variation ◽

Cytosine Methylation ◽

Callus Formation ◽

Expression Profiles ◽

Close Association ◽

Global Methylation ◽

Regeneration Efficiency ◽

The Impact

Oryza sativa indica (cv. IR64) and Oryza sativa japonica (cv. TNG67) vary in their regeneration efficiency. Such variation may occur in response to cultural environments that induce somaclonal variation. Somaclonal variations may arise from epigenetic factors, such as DNA methylation. We hypothesized that somaclonal variation may be associated with the differential regeneration efficiency between IR64 and TNG67 through changes in DNA methylation. We generated the stage-associated methylome and transcriptome profiles of the embryo, induced calli, sub-cultured calli, and regenerated calli (including both successful and failed regeneration) of IR64 and TNG67. We found that stage-associated changes are evident by the increase in the cytosine methylation of all contexts upon induction and decline upon regeneration. These changes in the methylome are largely random, but a few regions are consistently targeted at the later stages of culture. The expression profiles showed a dominant tissue-specific difference between the embryo and the calli. A prominent cultivar-associated divide in the global methylation pattern was observed, and a subset of cultivar-associated differentially methylated regions also showed stage-associated changes, implying a close association between differential methylation and the regeneration programs of these two rice cultivars. Based on these findings, we speculate that the differential epigenetic regulation of stress response and developmental pathways may be coupled with genetic differences, ultimately leading to differential regeneration efficiency. The present study elucidates the impact of tissue culture on callus formation and delineates the impact of stage and cultivar to determine the dynamics of the methylome and transcriptome in culture.

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