scholarly journals Assessing genetic diversity in a set of wheat (Triticum aestivum) genotypes using microsatellite markers to improve the yellow rust resistant breeding programs

2012 ◽  
Vol 7 (48) ◽  
pp. 6447-6455 ◽  
Author(s):  
Jamalirad S ◽  
A Mohammadi S ◽  
Toorchi M
Author(s):  
Ahmed Medhat Mohamed Al-Naggar ◽  
Mohamed Abd El-Maboud Abd El-Shafi ◽  
Mohamed Helmy El-Shal ◽  
Ali Hassan Anany

To increase the genetic progress in wheat (Triticum aestivum L.) yield, breeders search for germplasm of high genetic diversity, one of them is the landraces. The present study aimed at evaluating genetic diversity of 20 Egyptian wheat landraces and two cultivars using microsatellite markers (SSRs). Ten SSR markers amplified a total of 27 alleles in the set of 22 wheat accessions, of which 23 alleles (85.2%) were polymorphic. The majority of the markers showed high polymorphism information content (PIC) values (0.67-0.94), indicating the diverse nature of the wheat accessions and/or highly informative SSR markers used in this study. The genotyping data of the SSR markers were used to assess genetic variation in the wheat accessions by dendrogram. The highest genetic distance was found between G21 (Sakha 64; an Egyptian cultivar) and the landrace accession No. 9120 (G11). These two genotypes could be used as parents in a hybridization program followed by selection in the segregating generations, to identify some transgressive segregates of higher grain yield than both parents. The clustering assigned the wheat genotypes into four groups based on SSR markers. The results showed that the studied SSR markers, provided sufficient polymorphism and reproducible fingerprinting profiles for evaluating genetic diversity of wheat landraces. The analyzed wheat landraces showed a good level of genetic diversity at the molecular level. Molecular variation evaluated in this study of wheat landraces can be useful in traditional and molecular breeding programs.


2017 ◽  
Vol 1 (01) ◽  
pp. 46-51
Author(s):  
OUMER SHERIFF ◽  
KEFYALEW ALEMAYEHU

Sheriff O, Alemayehu K. 2017. Review: Genetic diversity studies using microsatellite markers and their contribution in supporting sustainable sheep breeding programs. Asian J Agric 1: 46-51. Microsatellites have been widely accepted and employed as useful molecular markers for measuring genetic diversity and divergence within and among populations. The various parameters developed so far to measure genetic diversity within and among populations are observed and expected heterozygosities (Ho and He), the mean number of alleles per locus (MNA),polymorphic information content (PIC), genetic distance and phylogenetic or tree building approach.The objective of thisreview was therefore to quantifythe genetic diversity studies of domestic sheep populations using microsatellite markersand their contribution in supporting sustainable sheep breeding programs. From the review, it is possible to see that there was high within population genetic variations in all the studied sheep populations, poor level of population differentiations and high levels of inbreeding. On the other hand, low estimates of hetrozygosities and mean number of alleles and employing only few and weak markers were observed in some of the studies. The gaps observed in the previous genetic diversity studies of the sheep populations may demand further works to reveal more information on the population structures andto start appropriate and sustainable breeding programs.


2012 ◽  
Vol 37 (3) ◽  
pp. 389-398 ◽  
Author(s):  
S Islam ◽  
MS Haque ◽  
RM Emon ◽  
MM Islam ◽  
SN Begum

A study was undertaken to examine the genetic diversity of 12 wheat (Triticum aestivum L.) genotypes, using 4 simple sequence repeats (SSRs). A total of 10 alleles were found. Allele number per locus ranged from 2 to 4 with an average of 2.5. The polymorphic information content (PIC) values ranged from 0.2755 to 0.5411 with an average of 0.3839. The average gene diversity over all SSR loci for the 12 wheat genotypes was 0.4688, ranging from 0.3299 to 0.6042. Cluster analysis based on microsatellite allelic diversity discriminated the varieties into different clusters. Genetic diversity was the highest between variety Gourab and Akbar as well as Gourab and BAW-1064, showing a genetic distance value of 0.4697. The genetic distance was lowest between Balaka and Aghrani as well as Triticale and BAW-1036. Positive correlations were found between gene diversity, number of alleles, the allele size range and the types of repeat motif of microsatellite markers. It was found from this study that microsatellite markers could characterize and discriminate all of the genotypes. More primers should be used for saturation of different regions in further studies. Bangladesh J. Agril. Res. 37(3): 389-398, September 2012 DOI: http://dx.doi.org/10.3329/bjar.v37i3.12082


Botany ◽  
2016 ◽  
Vol 94 (1) ◽  
pp. 9-13 ◽  
Author(s):  
Nader Rokni ◽  
Ebrahim Mohammadi Goltapeh ◽  
Alireza Shafeinia ◽  
Naser Safaie

Agaricus bisporus (Lange) Imbach is the most widely cultivated mushroom in Iran. Lack of diversity in mushroom crops, especially where disease is concerned, creates a crucial risk for the currently grown cultivars. The aim of this study was to assess the genetic diversity among Iranian wild strains and some commercial cultivars by using microsatellite markers. Eighteen codominant microsatellite markers of A. bisporus (AbSSR) were used to distinguish 17 wild and commercial strains. All of the microsatellite markers used in this research gave clear banding patterns, and only one strain remained undistinguished. Among 106 generated alleles, the wild subgroup presented 53 alleles never found both in brown and white commercial cultivars, and 42 alleles never found in commercial brown strains. The dendrogram obtained by UPGMA clustering analysis separated A. bisporus strains into six groups. Based on our results, the high level of genetic diversity among Iranian wild strains, compared with the commercial strains, provides a new and promising source of diversity for A. bisporus breeding programs. To our knowledge this is the first relevant study of biodiversity in native Iranian populations of A. bisporus.


Author(s):  
Izharullah Izharullah ◽  
Amin Ur Rehman ◽  
Zakiullah Zakiullah ◽  
Shahid Iqbal ◽  
Shehzad Shehzad ◽  
...  

2017 ◽  
Vol 16 (36) ◽  
pp. 1832-1839 ◽  
Author(s):  
Tekeu Honore ◽  
M. L. Ngonkeu Eddy ◽  
P. Djocgoue Francois ◽  
Ellis Aletta ◽  
Lendzemo Venasius ◽  
...  

2007 ◽  
Vol 54 (7) ◽  
pp. 1499-1506 ◽  
Author(s):  
Łukasz Stępień ◽  
Volker Mohler ◽  
Jan Bocianowski ◽  
Grzegorz Koczyk

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